[1]肖 麒,吴彦兵,陈 炼,等.中华绒螯蟹14-3-3ζ基因cDNA全长克隆与序列分析[J].南京师大学报(自然科学版),2021,44(04):69-75.[doi:10.3969/j.issn.1001-4616.2021.04.009]
　Xiao Qi,Wu Yanbing,Chen Lian,et al.cDNA Cloning,Characterization and Sequence Analyses of14-3-3ζ Gene in Eriocheir sinensis[J].Journal of Nanjing Normal University(Natural Science Edition),2021,44(04):69-75.[doi:10.3969/j.issn.1001-4616.2021.04.009]
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中华绒螯蟹14-3-3ζ基因cDNA全长克隆与序列分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第44卷
期数:
2021年04期
页码:
69-75
栏目:
·生态学·
出版日期:
2021-12-15

文章信息/Info

Title:
cDNA Cloning,Characterization and Sequence Analyses of14-3-3ζ Gene in Eriocheir sinensis
作者:
肖 麒1; 吴彦兵1; 陈 炼2; 李 鹏1
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.南京林业大学生物与环境学院,江苏 南京 210037)
Author(s):
Xiao Qi1; Wu Yanbing1; Chen Lian2; Li Peng1
(1.School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnology,Nanjing 210023,China)(2.College of Biology and the Environment,Nanjing Forestry University,Nanjing 210037,China)
关键词:
中华绒螯蟹; 14-3-3ζ; cDNA; 序列分析
Keywords:
Eriocheir sinensis; 14-3-3ζ; cDNA; sequence analyses
分类号:
Q958
DOI:
10.3969/j.issn.1001-4616.2021.04.009
文献标志码:
A
摘要:
14-3-3蛋白可以与不同蛋白质结合从而参与多种磷酸化调节的信号通路,并在生物代谢、信号转导、细胞周期控制、凋亡、蛋白质运输、转录、应激反应等方面发挥重要作用. 本文用SMART RACE技术克隆获得了中华绒螯蟹14-3-3ζ基因的全长cDNA序列,14-3-3ζ 基因cDNA全长为1 081 bp,包含一个744 bp的开放阅读框,其编码一个28.0 kDa的含247个氨基酸的多肽,编码的氨基酸序列包含保守的14-3-3结构域. 多序列比对、系统发生关系重建和其他生物信息学分析表明中华绒螯蟹14-3-3ζ为非分泌型蛋白,不具有跨膜区域,定位于细胞质中,其分子量与已知的其他物种的14-3-3ζ蛋白分子量相近. 中华绒螯蟹的14-3-3ζ序列与南美白对虾(Penaeus vannamei)、拟穴青蟹(Scylla paramamosain)等甲壳类动物的14-3-3ζ基因高度同源. 选择压力分析表明中华绒螯蟹14-3-3ζ受到纯化选择,其功能可能高度保守.
Abstract:
14-3-3 proteins can bind to different proteins to participate in a variety of phosphorylation regulatory signaling pathways,and play an important role in biological metabolism,signal transduction,cell cycle control,apoptosis,protein transport,transcription,stress response and other aspects. The full-length cDNA of 14-3-3ζ gene from the Chinese mitten crab Eriocheir sinensis was cloned and identified using SMART RACE technique. It is consisted of 1 081 nucleotides with an open-reading frame of 744 bp,which encoding 247 amino acids(28.0 kDa)and containing 14-3-3 domain. Sequence alignment,phylogenetic tree construction and some bioinformatics analyses revealed that 14-3-3ζ is non-secretory and no-transmembrane protein,which is probable located in cytoplasm. The molecular weight of 14-3-3ζ is similar than that of others 14-3-3ζ isoforms. The sequence of 14-3-3ζ in E.sinensis is highly homologous to that in the reported crustaceans such as Penaeus vannamei and Scylla paramamosain. Moreover,we observed a strong signature of purifying selection between the comparison of orthologous 14-3-3ζ gene pairs of E.sinensis. The results suggest a conservative function across crustaceans.

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备注/Memo

备注/Memo:
收稿日期:2021-01-15.
基金项目:国家自然科学基金项目(31770402、31000954).
通讯作者:李鹏,博士,副教授,研究方向:动物生态与进化生物学. E-mail:lipeng@njnu.edu.cn
更新日期/Last Update: 2021-12-15