[1]李淑贤,林 英,祁 雯,等.斑点福寿螺肠道菌群结构及功能研究[J].南京师大学报(自然科学版),2022,45(01):64-73.[doi:10.3969/j.issn.1001-4616.2022.01.010]
　Li Shuxian,Lin Ying,Qi Wen,et al.Research on the Bacterial Community Structure and Functions in the Intestine of Pomacea maculata[J].Journal of Nanjing Normal University(Natural Science Edition),2022,45(01):64-73.[doi:10.3969/j.issn.1001-4616.2022.01.010]
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斑点福寿螺肠道菌群结构及功能研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第45卷
期数:
2022年01期
页码:
64-73
栏目:
·生物学·
出版日期:
2022-03-15

文章信息/Info

Title:
Research on the Bacterial Community Structure and Functions in the Intestine of Pomacea maculata
文章编号:
1001-4616(2022)01-0064-10
作者:
李淑贤1; 2; 林 英1; 祁 雯3; 陈 燏3; 李雪霞2; 朱善良3; 卫 蔚3; 李 宏1; 陈 炼2
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.南京林业大学生物与环境学院,江苏 南京 210037)(3.江苏第二师范学院生命科学与化学学院,江苏 南京 210042)
Author(s):
Li Shuxian1; 2; Lin Ying1; Qi Wen3; Chen Yu3; Li Xuexia2; Zhu Shanliang3; Wei Wei3; Li Hong1; Chen Lian2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.College of Biology and Environment,Nanjing Forestry University,Nanjing 210037,China)(3.School of Life Sciences and Chemistry,Jiangsu Second Normal University,Nanjing 210042,China)
关键词:
肠道微生物; 高通量测序; 斑点福寿螺
Keywords:
intestinal microbiota; high-throughput sequencing; Pomacea maculata
分类号:
Q958
DOI:
10.3969/j.issn.1001-4616.2022.01.010
文献标志码:
A
摘要:
肠道微生物被认为是动物机体的共生体,在营养物质代谢、免疫调控等方面发挥重要作用. 入侵生物斑点福寿螺(Pomacea maculata)严重威胁农业生产和生态系统功能. 本研究利用高通量测序技术对11只雌、雄斑点福寿螺的肠道微生物多样性、结构和功能进行研究. 结果表明,在门水平上,斑点福寿螺肠道内容物主要优势菌为变形菌门(Proteobacteria)(43.52%)和软壁菌门(Tenericutes)(13.82%); 在属水平上,主要优势菌为气单胞菌属(Aeromonas)(15.66%)和支原体属(Mycoplasma)(7.26%). 雌、雄斑点福寿螺肠道微生物多样性和群落结构没有显著性差异(P>0.05). 在斑点福寿螺肠道内容物中检测到一些有益菌以及潜在致病菌. 基于PICRUSt分析预测斑点福寿螺肠道菌群功能,共得到24种代谢功能,其中氨基酸运输和代谢、通用功能预测、信号转导、细胞壁/膜、能量产生和转换丰度较大.
Abstract:
Intestine microbiome are considered as symbionts of animal bodies and play an important role in nutrient metabolism and immune regulation. Pomacea maculata is an invasive species that has caused serious damage to aquatic crop production and ecosystem functioning. In this study,high-throughput sequencing was used to analyze the intestinal microbial diversity,community structure and function of 11 female and male P.maculata. At the phylum level,Proteobacteria(43.52%)and Tenericutes(13.82%)were the dominant bacteria in the intestinal content microbiome of P.maculata. At the genus level,the dominant bacteria were Aeromonas(15.66%)and Mycoplasma(7.26%). No significant difference in intestinal microbial diversity and microbial community structure was found between male and female group(P>0.05). Some beneficial bacteria and potential pathogenic bacteria were found in the intestinal content microbiome of P.maculata. PICRUSt analysis was used to predict the intestinal microbiome functions of P.maculata,and 24 metabolic functions were predicted. The amino acid transport and metabolism,general function prediction only,transcription,cell wall/membrane/envelope biogenesis,energy production and conversion accounted for a large proportion.

参考文献/References:

[1] COWIE R H. Apple snails(Ampullariidae)as agricultural pests:their biology,impacts and management[M]. //BARKER G M(Ed.). Molluscs as crop pests. UK:CAB International,2002.
[2]HAYES K A,JOSHI R C,TIENGO S C,et al. Out of South America:multiple origins of non-native apple snails in Asia[J]. Diversity and distributions,2008,14(4):701-712.
[3]HAYES K A,COWIE R H,TIENGO S C,et al. Comparing apples with apples:clarifying the identities of two highly invasive Neotropical Ampullariidae(Caenogastropoda)[J]. Zoological journal of the linnean society,2012,166(4):723-753.
[4]BURKS R L,KYLE C H,TRAWICK M K. Pink eggs and snails:field oviposition patterns of an invasive snail,Pomacea insularum,indicate a preference for an invasive macrophyte[J]. Hydrobiologia,2010,646:243-251.
[5]HORGAN F G,STUART A M,KUDAVIDANAGE E P. Impact of invasive apple snails on the functioning and services of natural and managed wetlands[J]. Acta oecological,2014,54:90-100.
[6]MARTIN C W,VALENTINE J F. Tolerance of embryos and hatchlings of the invasive apple snail Pomacea maculata to estuarine conditions[J]. Aquatic ecology,2014,48:321-326.
[7]ADALLA C B,MAGSINO E A. Understanding the golden apple snail(Pomacea canaliculata):biology and early initiatives to control the pest in the Philippines[M]. //JOSHI R C,SEBASTIAN L S(Eds.). Global advances in ecology and management of golden apple snails. Nueva Ecija,Philippines:Philippines Rice Research Institute,2006.
[8]LV S,ZHANG Y,LIU H X,et al. Phylogenetic evidence for multiple and secondary introductions of invasive snails:Pomacea species in the People’s Republic of China[J]. Diversity & distributions,2013,19(2):147-156.
[9]杨倩倩,刘苏汶,茹炜岽,等. 基于DNA条形码技术对浙江省外来入侵福寿螺进行分子鉴定[J]. 生物多样性,2016,24(3):341-350.
[10]YANG Q Q,LIU S W,HE C,et al. Invisible apple snail invasions:importance of continued vigilance and rigorous taxonomic assessments[J]. Pest management science,2018,75(5):1277-1286.
[11]RAWLS J F,SAMUELL B S,GORDON J I. Gnotobiotic zebrafish reveal evolutionarily conserved responses to the gut microbiota[J]. Proceedings of the national academy of sciences of the United States of America,2004,101(13):4596-4601.
[12]FLINT H J,BAYER E A,RINCON M T,et al. Polysaccharide utilization by gut bacteria:potential for new insights from genomic analysis[J]. Nature reviews microbiology volume,2008,6(2):121-131.
[13]LEY R E,HAMADY M,LOZUPONE C,et al. Evolution of mammals and their gut microbes[J]. Science,2008,320(5883):1647-1651.
[14]许刚,孙振丽,胡小龙,等. 基于16SrRNA基因序列分析家蚕肠道细菌的多样性[J]. 蚕业科学,2015,41(4):641-649.
[15]MA R,WANG S,ZHAO F,et al. Comparative study on intestinal bacterial communities of Boleophthalmus pectinirostris and Periophthalmus magnuspinnatus with different sexes and feeding strategies[J]. Annals of microbiology,2018,68(3):123-33.
[16]程天印,曲凌玄,王晓君. 应用PCR-DGGE技术分析福寿螺胃肠道菌群[J]. 中国农学通报,2015,31(11):98-102.
[17]张盛静,赵小金,宋晓玲,等. 人工养殖对虾肠道内可培养细菌数量及组成分析[J]. 上海海洋大学学报,2015,24(2):211-218.
[18]CAPORASO J G,LAUBER C L,WALTERS W A,et al. Global patterns of 16s rRNA diversity at a depth of millions of sequences per sample[J]. Proceedings of the national academy of sciences of the United States of America,2011,108(Supplement 1):4516-4522.
[19]LI L H,LV S,LU Y,et al. Spatial structure of the microbiome in the gut of Pomacea canaliculata[J]. BMC microbiology,2019,19(1):273.
[20]HAO Y,GUAN W,WU H,et al. Intestinal microbiome profiles in Oncomelania hupensis in mainland China[J]. Acta tropica,2020,201:105202.
[21]胡宗福,常杰,佟庆,等. 高通量测序方法分析两种草食性淡水螺肠道菌群多样性[J]. 生物工程学报,2020,36(12):1-13.
[22]GODOY M S,CASTRO-VAZQUEZ A,VEGA I A. Endosymbiotic and host proteases in the digestive tract of the invasive snail Pomacea canaliculata:diversity,origin and characterization[J]. PLoS one,2013,8(6):e66689.
[23]YANG Q,LIU S,HE C,et al. Invisible apple snail invasions:importance of continued vigilance and rigorous taxonomic assessments[J]. Pest management science,2019,75(5):1277-1286.
[24]XU N,TAN G,WANG H,et al. Effect of biochar additions to soil on nitrogen leaching,microbial biomass and bacterial community structure[J]. European journal of soil biology,2016,74:1-8.
[25]WANG X,SUN G,FENG T,et al. Sodium oligomannate therapeutically remodels gut microbiota and suppresses gut bacterial amino acids-shaped neuroinflammation to inhibit Alzheimer’s disease progression[J]. Cell research,2019,29(7):1-17.
[26]WANG Q,GARRITY G M,TIEDJE J M,et al. Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy[J]. Applied and environmental microbiology,2007,73(16):5261-5267.
[27]刘小改,杨亚军,廖秋菊,等. 稻纵卷叶螟肠道细菌群落结构与多样性分析[J]. 昆虫学报,2016,59(9):965-976.
[28]LANGILLE M G,ZANEVELD J,CAPORASO J G,et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J]. Nature biotechnology,2013,31(9):814-821.
[29]TAKACS-VESBACH C,KING K,VAN HORN D,et al. Distinct bacterial microbiomes in sexual and asexual Potamopyrgus antipodarum,a new zealand freshwater snail[J]. PLoS one,2016,11(8):e0161050.
[30]JOYNSON R,PRITCHARD L,OSEMWEKHA E,et al. Metagenomic analysis of the gut microbiome of the common black slug Arion ater in search of novel lignocellulose degrading enzymes[J]. Frontiers in microbiology,2017,8:2181.
[31]GONG M F,GUAN Q L,OU X F,et al. Screening and production of cellulase of bacteria isolated from intestinal tract of Pomacea canaliculata[J]. Journal of biobased materials and bioenergy,2017,11(4):278-281.
[32]MORRISON M,POPE P B,DENMAN S E,et al. Plant biomass degradation by gut microbiomes:more of the same or something new?[J]. Current opinion in biotechnology,2009,20(3):358-363.
[33]RIMOLDI S,TEROVA G,ASCIONE C,et al. Next generation sequencing for gut microbiome characterization in rainbow trout(Oncorhynchus mykiss)fed animal by-product meals as an alternative to fishmeal protein sources[J]. PLoS one,2018,13(3):e0193652.
[34]HU Z,CHEN X,CHANG J,et al. Compositional and predicted functional analysis of the gut microbiota of Radix auricularia(Linnaeus)via high-throughput Illumina sequencing[J]. Journal of peer,2018,6:e5537.
[35]QIAO F,LEI K,LI Z,et al. Transcriptomic responses of the freshwater snail(Parafossarulus striatulus)following dietary exposure to cyanobacteria[J]. Science of the total environment,2018,624(15):153-161.
[36]YE L,AMBERG J,CHAPMAN D,et al. Fish gut microbiota analysis differentiates physiology and behavior of invasive Asian carp and indigenous American fish[J]. The ISME journal,2014,8(3):541-551.
[37]JIANG Y,XIE C,YANG G,et al. Cellulase-producing bacteria of Aeromonas are dominant and indigenous in the gut of Ctenopharyngodon idellus(Valenciennes)[J]. Aquaculture research,2011,42(4):499-505.
[38]CEYLAN E,BERKTAS M,AGAOGLU Z. The occurrence and antibiotic resistance of motile Aeromonas in livestock[J]. Tropical animal health and production,2009,41(2):199-204.
[39]HUANG Z B,GUO F,ZHAO J,et al. Molecular analysis of the intestinal bacterial flora in cage-cultured adult small abalone,Haliotis diversicolor[J]. Aquaculture research,2010,41:e760-e769.
[40]ARONSON H S,ZELLMER A J,GOFFREDI S K. The specific and exclusive microbiome of the deep-sea bone-eating snail,Rubyspira osteovora[J]. FEMS microbiology ecology,2016,93:fiw250.
[41]SEBASTEN D,MARIE-ANNE P,NELLY L,et al. A tale of two chitons:is habitat specialisation linked to distinct associated bacterial communities?[J]. FEMS microbiology ecology,2013,83:552-567.
[42]RAZIN S,YOGEV D,NAOT Y. Molecular biology and pathogenicity of mycoplasmas[J]. Microbiology and molecular biology reviews,1998,62:1094-1156.
[43]TSUCHIYA C,SAKATA T,SUQITA H. Novel ecological niche of Cetobacterium somerae,an anaerobic bacterium in the intestinal tracts of freshwater fish[J]. Letters in applied microbiology,2008,46(1):43-48.
[44]王玉堂. 蛭弧菌及其在水产养殖动物疾病防治中的应用[J]. 中国水产,2014,2:44-47.
[45]陈家长,简纪常,胡庚东,等. 利用有益微生物改善养殖生态环境的研究[J]. 湛江海洋大学学报,2002,22(4):33-36.
[46]CARDOSO A M,CAVALCANTE J J V,CANT? M E,et al. Metagenomic analysis of the microbiota from the crop of an invasive snail reveals a rich reservoir of novel genes[J]. PLoS one,2012,7(11):e48505.
[47]尹绍武,颜亨梅,王洪全. 福寿螺的生物学研究[J]. 湖南师范大学自然科学学报,2000,23(2):76-82.
[48]MATSUKURA K,IZUMI Y,YOSHIDA K,et al. Cold tolerance of invasive freshwater snails,Pomacea canaliculata,P.maculata,and their hybrids helps explain their different distributions[J]. Freshwater biology,2016,61(1):80-87.
[49]PAWAR K D,BANSKAR S,RANE S D,et al. Bacterial diversity in different regions of gastrointestinal tract of giant African snail(Achatina fulica)[J]. Microbiologyopen,2012,1(4):415-426.
[50]NICOLAI A,ROULAND-LEFEVRE C,ANSART A,et al. Inter-population differences and seasonal dynamic of the bacterial gut community in the endangered land snail Helix pomatia(Gastropoda:Helicidae)[J]. Malacologia,2015,59(1):177-190.
[51]沈佳斌,张雪敬,吴蔚,等. 雉科鸟类肠道微生物的多样性与功能及其对食性的适应[J]. 南京师大学报(自然科学版),2016,39(2):90-95.

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备注/Memo

备注/Memo:
收稿日期:2021-01-14.
基金项目:国家自然科学基金项目(32170434)、江苏省自然科学基金项目(BK20171407).
通讯作者:陈炼,博士,副教授,研究方向:入侵生物学. E-mail:chenlian_2004@163.com
更新日期/Last Update: 1900-01-01