[1]杨子萍,李大命,刘燕山,等.基于线粒体控制区D-loop序列的洪泽湖翘嘴鲌遗传多样性分析[J].南京师大学报(自然科学版),2023,46(02):70-76.[doi:10.3969/j.issn.1001-4616.2023.02.009]
　Yang Ziping,Li Daming,Liu Yanshan,et al.Genetic Diversity Analysis of Culter alburnus in Hongze Lake Based on Mitochondrial Control Region D-loop Sequence[J].Journal of Nanjing Normal University(Natural Science Edition),2023,46(02):70-76.[doi:10.3969/j.issn.1001-4616.2023.02.009]
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基于线粒体控制区D-loop序列的洪泽湖翘嘴鲌遗传多样性分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第46卷
期数:
2023年02期
页码:
70-76
栏目:
生物学
出版日期:
2023-06-15

文章信息/Info

Title:
Genetic Diversity Analysis of Culter alburnus in Hongze Lake Based on Mitochondrial Control Region D-loop Sequence
文章编号:
1001-4616(2023)02-0070-07
作者:
杨子萍1; 2; 李大命1; 刘燕山1; 谷先坤1; 杨家新2
(1.南京师范大学海洋科学与工程学院,江苏 南京 210023)
(2.江苏省淡水水产研究所,江苏省内陆水域渔业资源重点实验室,江苏 南京 210017)
Author(s):
Yang Ziping1; 2; Li Daming1; Liu Yanshan1; Gu Xiankun1; Yang Jiaxin2
(1.College of Marine Science and Engineering,Nanjing Normal University,Nanjing 210023,China)
(2.Freshwater Fisheries Research Institute of Jiangsu Province,Key Laboratory of Fisheries Resources in Inland Waters of Jiangsu Province,Nanjing 210017,China)
关键词:
翘嘴鲌; 控制区序列; 遗传多样性; 洪泽湖
Keywords:
Culter alburnus;  mtDNA control region;  genetic diversity;  Hongze Lake
分类号:
S917
DOI:
10.3969/j.issn.1001-4616.2023.02.009
文献标志码:
A
摘要:
为掌握洪泽湖翘嘴鲌(Culter alburnus)种质资源遗传多样性状况,采集洪泽湖6个群体共233尾翘嘴鲌,采用线粒体控制区D-loop全序列进行遗传多样性分析. 结果显示,翘嘴鲌线粒体D-loop序列长度为934 bp～936 bp,碱基(A+T)含量(66.0%)明显高于(G+C)含量(34.0%). 243条D-loop序列检出23个变异位点,其中有9个单一信息位点,14个简约信息位点. 6个群体发现22个单倍型,全部群体的单倍型多样性(Hd)和核苷酸多样性(Pi)分别为(0.794±0.019)和(0.002 51±0.000 14),呈现出高Hd和低Pi的遗传多样性模式. 6个群体中洪泽湖群体的遗传多样性最高(Hd:0.873±0.038,Pi:0.002 95±0.000 35),顾勒河群体的遗传多样性最低(Hd:0.709±0.064,Pi:0.002 03±0.000 31). 分子方差分析表明,群体间的分子变异占比0.43%,群体内的分子变异占比 99.57%,分子变异主要发生在群体内. 群体间遗传分化指数Fst为-0.019 12～0.028 01(P>0.05),表明群体间没有出现遗传分化. 单倍型系统进化树和单倍型网络结构图显示,群体间拥有多个共享单倍型,单倍型聚为一个进化分支,没有形成特定的地理遗传结构. 中性检验结果表明洪泽湖翘嘴鲌群体没有经历显著种群扩张事件. 研究结果表明,洪泽湖翘嘴鲌遗传多样性较低,6个群体间基因交流频繁,遗传分化不显著,可将6个群体作为一个管理单元进行保护,需采取措施提高翘嘴鲌遗传多样性及恢复其种群数量.
Abstract:
In order to understand the present genetic diversity of Culter alburnus populations in Hongze Lake, we sampled a total of 233 C. alburnus samples from 6 geographic populations and conducted genetic diversity analysis based on sequences of D-loop control region. The results showed that the full length of D-loop sequences was 934 bp-936 bp, (A+T)base composition(66.0%)was higher than the(C+G)combination(34.0%). A total of 23 variable sites were identified in 243 D-loop sequences in which 9 singleton mutation and 14 parsimony informative sites. 22 haplotypes were defined in 6 populations. The overall haplotype diversity index(Hd)was(0.794±0.019)and the overall nucleic acid diversity index(Pi)was(0.002 51±0.000 14), which showed high Hd and low π genetic pattern. The highest and lowest index value of haplotype diversity and nucleic acid diversity were identified in Hongze popluation(Hd:0.873±0.038, Pi:0.002 95±0.000 35)and Gule River population(Hd:0.709±0.064, Pi:0.002 03±0.000 31), respectively. The result of AMOVA demonstrated that 0.43% molecular variation was among populations and 99.57% within populations, and molecular variation mainly occurred within populations. The values of pairwise genetic differentiation index were -0.019 12-0.028 01, which revealed that there were no sigificant genetic differences among pouplations(P>0.05). The NJ phylogenetic tree and network structure diagram of haplotypes showed that there were more shared haplotypes among populations, and haplotypes were clustered into one clade without forming a specific geographic genetic structure. The results of the neutrality test revealed that no population expansion happened in evolutionary history of C. alburnus. In conclusion, the genetic diversity of C. alburnus was low, and the 6 populations can be viewed as a management unit. Furthermore, we should take measures to improve its genetic diversity and population size of C. alburnus in Hongze Lake.

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备注/Memo

备注/Memo:
收稿日期:2022-10-16.
基金项目:江苏省农业科技自主创新项目(CX(21)3003)、2021和2022年度省级单位项目(2021-SJ-110-03; 2022-SJ-061-02).
通讯作者:李大命,博士,研究员,研究方向:渔业种质资源监测和评估. E-mail:ldm8212@126.com
更新日期/Last Update: 2023-06-15