[1]黄馨阅,丁慧敏,马士玄,等.红须腹菌多糖的提取、羧甲基化修饰及免疫活性研究[J].南京师大学报(自然科学版),2023,46(03):60-68.[doi:10.3969/j.issn.1001-4616.2023.03.009]
　Huang Xinyue,Ding Huimin,Ma Shixuan,et al.Extraction,Carboxymethylation Modification and in Vivo Immunomodulatory Effects of Polysaccharides from Rhizopogon Rubescens(Tul.)Tul[J].Journal of Nanjing Normal University(Natural Science Edition),2023,46(03):60-68.[doi:10.3969/j.issn.1001-4616.2023.03.009]
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红须腹菌多糖的提取、羧甲基化修饰及免疫活性研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第46卷
期数:
2023年03期
页码:
60-68
栏目:
生物学
出版日期:
2023-09-15

文章信息/Info

Title:
Extraction,Carboxymethylation Modification and in Vivo Immunomodulatory Effects of Polysaccharides from Rhizopogon Rubescens(Tul.)Tul
文章编号:
1001-4616(2023)03-0060-09
作者:
黄馨阅; 丁慧敏; 马士玄; 王秋艳; 陶明煊
(南京师范大学食品制药与工程学院,江苏 南京 210023)
Author(s):
Huang Xinyue; Ding Huimin; Ma Shixuan; Wang Quiyan; Tao Mingxuan
(School of Food Science and Pharmaceutical Engineering,Nanjing Normal University,Nanjing 210023,China)
关键词:
红须腹菌; 多糖; 羧甲基化修饰; 免疫调节活性
Keywords:
Rhizopogon rubescens(Tul.)Tul;  polysaccharide;  carboxymethylation modification;  immunomodulatory activity
分类号:
TS201.2
DOI:
10.3969/j.issn.1001-4616.2023.03.009
文献标志码:
A
摘要:
红须腹菌(Rhizopogon rubescens(Tul.)Tul)是一类主要分布于中国云南省及福建省的根须腹菌属食用菌. 本文以红须腹菌为原料提取红须腹菌多糖,再加以羧甲基化修饰,并通过体内动物实验对两种多糖的免疫活性进行比较研究. 采用水提醇沉法提取红须腹菌多糖,并通过氢氧化钠-氯乙酸体系对多糖进行羧甲基化修饰. 以小鼠血清细胞因子含量,血细胞数量,单核巨噬细胞吞噬能力及脏器组织形态作为指标评价两种多糖的免疫活性. 研究结果表明,红须腹菌多糖总糖含量为92.06%,羧甲基的取代度为0.352. 红须腹菌多糖及羧甲基多糖均对环磷酰胺造成的小鼠脏器指数下降,部分血清细胞因子含量减少,造血功能及巨噬细胞吞噬能力减弱,以及脾脏和胸腺的组织损伤有较为显著的缓解作用,其中以羧甲基多糖效果更佳. 这表明红须腹菌多糖及羧甲基化多糖均具有一定的免疫调节功效,且羧甲基化多糖的体内免疫活性更为显著.
Abstract:
Rhizopogon rubescens(Tul.)Tul is a kind of edible fungi of the Rhizopogon,which is mainly distributed in Yunnan and Fujian provinces of China. The primary polysaccharide fraction from Rhizopogon rubescens(Tul.)Tul(PPRR)was extracted by ultrasonic-assisted hot water extraction. Then the PPRR was modified by carboxymethylation to obtain carboxymethyl polysaccharide(CM-PPRR),and the immune activities of the two polysaccharides were compared by animal experiments in vivo. The immune activities of the two polysaccharides were evaluated by the content of serum cytokines,the number of blood cells,the phagocytic ability of mononuclear macrophages and the morphology of organs. The results show that the total sugar content of polysaccharide was 92.06%,and the degree of substitution of carboxymethyl was 0.352. In vivo experiments show that both the PPRR and CM-PPRR can significantly alleviate the decline of organ index,the content of some serum cytokines,the weakening of hematopoietic function and macrophage phagocytosis,and the tissue damage of spleen and thymus caused by cyclophosphamide(CPA),especially CM-PPRR. It showed that both PPRR and CM-PPRR had certain immunomodulatory effects,and CM-PPRR had more significant immune activity in vivo.

参考文献/References:

[1]才晓玲. 常见食用菌简介[M]. 北京:中国农业大学出版社,2018:180-181.
[2]刘江英. 锁阳多糖体外免疫活性及多糖提取工艺优化研究[D]. 内蒙古:内蒙古大学,2019.
[3]罗婷.苹果渣多糖的羧甲基化修饰及其抗氧化活性研究[D]. 西安:陕西科技大学,2019.
[4]江连洲,张巧智,关嘉琦,等. 羧甲基化大豆水酶法膳食纤维的制备及其性能研究[J]. 农业机械学报,2017,48(10):330-337.
[5]朱亚男,徐菘阳,丁慧敏,等. 白玉菇多糖对免疫抑制型小鼠的免疫调节作用[J]. 食品工业科技,2020,41(7):295-300,308.
[6]LI X,GAO J,YU Z,et al. Regulatory effect of anwulignan on the immune function through its antioxidation and anti-apoptosis in D-galactose-induced aging mice[J]. Clinical interventions in aging,2020,15:97-110.
[7]陈栅,冯润芳,袁野,等. 酸枣多糖羧甲基化修饰及活性研究[J]. 中国食品学报,2022,22(4):55-66.
[8]HUANG S Y,HUANG G L. Extraction,structural analysis and activities of rice bran polysaccharide[J]. Chemical biology & drug design,2021,98(4):631-638.
[9]卜丹丹. 玉米皮多糖羧甲基化和硫酸酯化改性的活性研究[D]. 天津:天津科技大学,2016.
[10]许春平,孙懿岩,白家峰,等. 怀山药多糖的羧甲基化修饰及保润性能[J]. 河南科技大学学报(自然科学版),2019,40(6):70-74,78-79.
[11]DUAN S,ZHAO M,WU B,et al. Preparation,characteristics,and antioxidant activities of carboxymethylated polysaccharides from blackcurrant fruits[J]. International journal of biological macromolecules,2020,155:1114-1122.
[12]XU X J,CHEN P,ZHANG L N,et al. Chain structures of glucans from Lentinus edodes and their effects on NO production from RAW 264.7 macrophages[J]. Carbohydrate polymers,2012,87:1855-1862.
[13]CHEN S P,LIU C C,HUANG X J,et al. Comparison of immunomodulatory effects of three polysaccharide fractions from Lentinula edodes water extracts[J]. Journal of functional foods,2020,66,103791.
[14]CHEN Y G,SHEN Z J,CHEN X P. Evaluation of free radicals scavenging and immunity-modulatory activities of Purslane polysaccharides[J]. International journal of biological macromolecules,2009,45(5):448-452.
[15]LI X,GAO J,YU Z,et al. Regulatory effect of anwulignan on the immune function through its antioxidation and anti-apoptosis in D-galactose-induced aging mice[J]. Clinical interventions in aging,2020,15:97-110.
[16]WU X,LIU Z,LIU Y,et al. Immunostimulatory effects of polysaccharides from Spirulina platensis in vivo and vitro and their activation mechanism on RAW246.7 macrophages[J]. Marine drugs,2020,18(11):538.
[17]FU Y F,JIANG L H,ZHAO W D,et al. Immunomodulatory and antioxidant effects of total flavonoids of Spatholobus suberectus Dunn on PCV2 infected mice[J]. Scientific reports,2017,7(1):8676.
[18]石和元,桑红灵,谭爱华,等. 山茱萸多糖对免疫抑制小鼠免疫功能的影响[J]. 现代免疫学,2022,42(6):482-488.
[19]王趁芳,邓娟,韩玉皎,等. 猴头菇多糖对免疫应激小鼠胸腺和脾脏显微结构、免疫功能及细胞增殖和凋亡的影响[J]. 中国食品卫生杂志,2022,34(3):482-490.
[20]GUO M Z,MENG M,FENG C C,et al. A novel polysaccharide from Craterellus cornucopioides enhances immunomodulatory activity on immunosuppressive mice models by regulation of TLR4-NF-κB pathway[J]. Food & function,2019,10(8):4792-4801.
[21]LIANG R J. Orthogonal test design for optimization of the extraction of polysaccharides from Phascolosoma esulenta and evaluation of its immunity activity[J]. Carbohydrate polymers,2008,73(4):318-325.
[22]YANG G K,LIANG X M,HU J H,et al. Feeding tea polysaccharides affects lipid metabolism,antioxidant capacity and immunity of common carp(Cyprinus carpio L.)[J]. Frontiers in immunology,2022,13:1074198.
[23]ZHOU B X,NGUYEN A D,SHEN J Y,et al. Effects of Caulerpa lentillifera polysaccharides on immune function in vitro and in vivo[J]. Journal of food processing and preservation,2022,46(12):e17260.
[24]黄娟,黄金莉,孙佳悦,等. 岩藻多糖对免疫低下小鼠模型免疫功能和肠道菌群的调节作用[J]. 中国食品学报,2022,22(5):92-102.
[25]刘思美,赵鹏,张婷婷,等. 地黄硒多糖的合成、表征及免疫活性分析[J]. 中国中药杂志,2022,47(11):2938-2946.
[26]XU X F,YAN H D,TANG J,et al. Polysaccharides in lentinus edodes:isolation,structure,immunomodulating activity and future prospective[J]. Critical reviews in food science and nutrition,2013,54(4):474-487.
[27]吴雨龙,朱华,张艺鏻,等. 菊苣多糖对免疫抑制小鼠免疫功能的影响[J]. 食品工业科技,2021,42(3):284-289,337.
[28]黄振华,邓向亮,张凯敏,等. 枸杞多糖对免疫抑制小鼠红细胞免疫功能的影响[J]. 中国免疫学杂志,2018,34(2):214-217.
[29]甘霓,许海林,吴小勇,等. 黑木耳多糖AAP-10对免疫抑制小鼠的免疫调节作用[J]. 食品科学,2018,39(19):196-200.
[30]高笑笑,王刚,杨歆睿,等. 雪灵芝粗多糖对环磷酰胺致免疫抑制小鼠免疫功能的影响[J]. 免疫学杂志,2017,33(3):191-196.
[31]WANG Y,JIN H,YU J,et al. Quality control and immunological activity of lentinan samples produced in China[J]. International journal of biological macromolecules,2020,159:129-136.
[32]CHEN X,NIE W,FAN S,et al. A polysaccharide from Sargassum fusiforme protects against immunosuppression in cyclophosphamide-treated mice[J]. Carbohydrate polymers,2012,90(2):1114-1119.

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备注/Memo

备注/Memo:
收稿日期:2023-01-06.
基金项目:浙江省动物蛋白食品精深加工技术重点实验室开放基金项目.
通讯作者:陶明煊,硕士,副教授,研究方向:生物活性物质及保健功能因子. E-mail:45017@njnu.edu.cn
更新日期/Last Update: 2023-09-15