[1]王炜祺,宋怡霖,薛雯蔚,等.基于HPLC指纹图谱及模式识别的生脉饮热稳定性评价[J].南京师大学报(自然科学版),2024,(01):14-20.[doi:10.3969/j.issn.1001-4616.2024.01.003]
 Wang Weiqi,Song Yilin,Xue Wenwei,et al.Evaluation of Thermal Stability of Shengmai Yin Based on HPLC Fingerprinting and Pattern Recognition[J].Journal of Nanjing Normal University(Natural Science Edition),2024,(01):14-20.[doi:10.3969/j.issn.1001-4616.2024.01.003]
点击复制

基于HPLC指纹图谱及模式识别的生脉饮热稳定性评价()
分享到:

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
期数:
2024年01期
页码:
14-20
栏目:
化学
出版日期:
2024-03-15

文章信息/Info

Title:
Evaluation of Thermal Stability of Shengmai Yin Based on HPLC Fingerprinting and Pattern Recognition
文章编号:
1001-4616(2024)01-0014-07
作者:
王炜祺12宋怡霖1薛雯蔚1张长丽1张边江1陈昌云1
(1.南京晓庄学院环境科学学院,江苏 南京 211171)
(2.江苏卫生健康职业学院药学院,江苏 南京 211800)
Author(s):
Wang Weiqi12Song Yilin1Xue Wenwei1Zhang Changli1Zhang Bianjiang1Chen Changyun1
(1.College of Environmental Science,Nanjing Xiaozhuang University,Nanjing 211171,China)
(2.College of Pharmacy,Jiangsu Health Vocational College,Nanjing 211800,China)
关键词:
生脉饮高效液相色谱指纹图谱热稳定性评价
Keywords:
Shengmai Yinhigh performance liquid chromatographyfingerprintthermal stability evaluation
分类号:
R917
DOI:
10.3969/j.issn.1001-4616.2024.01.003
文献标志码:
A
摘要:
构建生脉饮的高效液相指纹图谱,综合运用化学模式识别技术,对其进行热稳定性评价. 通过色谱图的比对分析,筛选出最优的实验条件. 采用WondaSil C18(4.6 mm×150 mm,5 μm)作为色谱柱; 以0.2%甲酸-乙腈为流动相,梯度洗脱,检测波长为260 nm,流速为1.0 mL/min,柱温为30 ℃. 采用SPSS、SIMCA等数据分析软件进行模式识别. 构建18份供试品溶液的指纹图谱,确定了8个共有峰,并指认出4个主要成分,分别是5-羟甲基糠醛、党参炔苷、原儿茶酸和五味子醇甲. 指纹图谱的相似度达到0.828及以上. 此方法简便,稳定,重复性良好,能有效识别生脉饮中的化学成分,为生脉饮质量控制及热稳定性提供参考. 随温度升高,峰面积减小,五味子醇甲的热稳定性降低,且不同厂家的生脉饮中五味子醇甲的热稳定性有所差异,其他成分热稳定性良好.
Abstract:
The objective of this study was to establish a comprehensive fingerprint profile of Shengmai Yin and to evaluate its thermal stability using a combination of chemical pattern recognition techniques. Optimal experimental conditions were determined by comparing and analyzing chromatograms. The chromatographic column employed WondaSil C18(4.6 mm×150 mm,5 μm),with a mobile phase consisting of 0.2% formic acid solution mixed with acetonitrile. Gradient elution was employed,with a detection wavelength of 260 nm,a flow rate of 1.0 mL/min,and a column temperature of 30 ℃. Data analysis software,including SPSS and SIMCA,was utilized for chemical pattern recognition analysis. The construction of fingerprint spectra for 18 test solution samples facilitated the identification of 8 common peaks and 4 major constituents,namely 5-hydroxymethylfurfural,codonoside,protocatechuic acid and schisandrin. The similarity of the fingerprint spectra reached 0.828. The method is simple,stable and reproducible. The chemical constituents of Shengmai Yin are preliminarily clarified,which can provide reference for the quality control and thermal stability of Shengmai Yin. The findings revealed that as the temperature increased,the peak area decreased,indicating a decrease in the thermal stability of Schisandrin A. It was observed that the thermal stability of Schisandrin A in Shengmai Yin varied among different manufacturers. Conversely,the other components in Shengmai Yin exhibited good thermal stability.

参考文献/References:

[1]窦金金,张喜武,王璐璇,等. 以有效相态为视角的生脉饮有效迭代相态研究及药效学验证[J]. 中草药,2021,52(4):993-999.
[2]崔海峰,彭博,冯淑怡,等. 生脉饮对阿霉素损伤心肌细胞的保护作用研究[J]. 中国中医基础医学杂志,2022,28(8):1245-1248,1273.
[3]袁亚慧,袁蓉,缪宇,等. 黄芪生脉饮对疲劳合并心肌损伤大鼠模型的作用及相关机制[J]. 中国中药杂志,2022,47(19):5292-5298.
[4]习涛,程泽君,邢新军,等. 中西医结合在急诊治疗重症心力衰竭中的优势及对心脏射血能力的影响[J]. 科技导报,2022,40(23):30-36.
[5]崔海峰,彭博,冯淑怡,等. 生脉饮对阿霉素损伤心肌细胞的保护作用研究[J]. 中国中医基础医学杂志,2022,28(8):1245-1248.
[6]窦建林,何龙平,钟林翠,等. 生脉饮对劳力性热射病大鼠凝血功能障碍的预防作用[J]. 解放军医学杂志,2023,48(7):790-796.
[7]AN X D,MAO L N,XIA P,et al. Effects of Shengmai Yin on pulmonary and cardiac function in coronavirus disease 2019 convalescent patients with cardiopulmonary symptoms:a randomized,double blind,multicenter control trial[J]. Journal of traditional Chinese medicine,2023,43(1),140-145.
[8]周凯旋,张亚杰,关婉辰,等. 基于整合药理学平台分析生脉饮人参方与党参方差异[J]. 中华中医药学刊,2021,39(2):151-155,275.
[9]逄世峰,许世泉,崔丽丽,等. 加糖红参对生脉饮质量的影响[J]. 中成药,2014,36(2):362-366.
[10]颜继忠,陈岑,楚楚,等. 生脉饮(党参方)中5-羟甲基糠醛的分离及其来源探究[J]. 浙江工业大学学报,2015,43(4):379-382.
[11]曹雨晴,楚尧娟,刘克锋,等. 我国中药指纹图谱研究的可视化分析[J]. 世界科学技术-中医药现代化,2020,22(9):3073-3081.
[12]李世雄,张喜利,刘文龙,等. 一种中药指纹图谱的定性定量研究新方法:全成分斜率相似度比较方法[J]. 时珍国医国药,2021,32(12):3064-3066.
[13]黄娜娜,李俊,饶高雄,等. 基于HPLC指纹图谱结合多种化学模式识别评价生脉饮(党参方)质量[J]. 中国医院药学杂志,2021,41(23):2433-2442.
[14]党晓月,朱志军,王永祥,等. 生脉饮HPLC指纹图谱与化学模式识别研究[J]. 中药材,2020,43(12):2988-2991.
[15]楚楚,徐绍静,吴琼琼,等. HPLC同时测定生脉饮(党参方)中多组分的含量[J]. 中药材,2013,36(5):820-823.
[16]支旭然,张黎媛,赵娜,等. UPLC同时测定生脉饮口服液中五味子醇甲、五味子醇乙、五味子甲素的含量[J]. 中国现代应用药学,2021,38(4):426-429.
[17]马雨璇,王娜,王冰,等. 抗凝药利伐沙班晶型的定量分析与热稳定性考察[J]. 南京师大学报(自然科学版),2021,44(1):136-140.
[18]王文平,吴慧敏,刘曼婷,等. 南葶苈子中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷的稳定性考察[J]. 中华中医药杂志,2020,35(11):5739-5742.
[19]邱越,赵春草,吴飞,等. 术桔和胃方浓缩过程中热稳定性考察[J]. 中国新药杂志,2019,28(1):104-109.
[20]XU L L,SHANG Z P,TIAN Y G,et al. Chemical variations among shengmaisan-based TCM patent drugs by ultra-high performance liquid chromatography coupled with hybrid quadrupole orbitrap mass spectrometry[J]. Molecules,2021,26(13):4000.
[21]杜绍洪,李文烁,郑江溢,等. 基于BP神经网络与聚类分析的数学创新能力研究[J]. 南京师大学报(自然科学版),2019,42(2):23-29.
[22]肖艺欣,朱锦懋,陈由强,等. 青钱柳叶片总黄酮含量测定及代谢组学分析[J]. 福建师范大学学报(自然科学版),2022,38(6):60-68.
[23]陈飞玥,朱玉莲,陈晓红. 多层特征融合的PCANet及其在人脸识别中的应用[J]. 南京师大学报(自然科学版),2021,44(2):127-133.
[24]覃柳,莹梁丽,金胡懿. 基于UPLC指纹图谱和网络药理学的当归炮制前后差异质量标志物研究[J]. 中草药,2023,54(18):5892-5903.
[25]杨金,陈林,周强,等. 基于数据的K均值理论及其应用[J]. 南京师大学报(自然科学版),2021,44(2):10-17.

相似文献/References:

[1]王为中,崔世海,闫婷婷,等.磁固相萃取与高效液相色谱联用测定水体中防治白蚁农药残留[J].南京师大学报(自然科学版),2016,39(04):0.[doi:10.3969/j.issn.1001-4616.2016.04.022]
 Wang Weizhong,Cui Shihai,Yan Tingting,et al.Combination Technology of Magnetic Solid-Phase Extraction andHigh Performance Liquid Chromatography and Its Application inDetection of Termiticides Residues in Aqueous Solutions[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(01):0.[doi:10.3969/j.issn.1001-4616.2016.04.022]

备注/Memo

备注/Memo:
收稿日期:2023-10-20.
基金项目:国家自然科学基金项目(22277057)、江苏卫生健康职业学院科研课题项目(JKB202201).
通讯作者:陈昌云,博士,教授,研究方向:分析化学计量学教学与科研.E-mail:1514470185@qq.com
更新日期/Last Update: 2024-03-15