[1]陈 燏,何鑫妮,常婷婷,等.微塑料对入侵种红耳滑龟和土著种中华草龟肠道菌群的影响[J].南京师大学报(自然科学版),2025,48(01):56-65.[doi:10.3969/j.issn.1001-4616.2025.01.008]
　Chen Yu,He Xinni,Chang Tingting,et al.Comparative Study of Responses to Microplastics in Alien Invasive Trachemys scripta elegans and Native Chinemys reevesii[J].Journal of Nanjing Normal University(Natural Science Edition),2025,48(01):56-65.[doi:10.3969/j.issn.1001-4616.2025.01.008]
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微塑料对入侵种红耳滑龟和土著种中华草龟肠道菌群的影响()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
48
期数:
2025年01期
页码:
56-65
栏目:
生态学
出版日期:
2025-02-15

文章信息/Info

Title:
Comparative Study of Responses to Microplastics in Alien Invasive Trachemys scripta elegans and Native Chinemys reevesii
文章编号:
1001-4616(2025)01-0056-10
作者:
陈 燏1; 何鑫妮2; 常婷婷2; 高 朔2; 3; 李 宏2
(1.江苏第二师范学院,江苏 南京 210013)
(2.南京师范大学生命科学学院,江苏 南京 210023)
(3.生态环境部南京环境科学研究所,江苏 南京 210042)
Author(s):
Chen Yu1; He Xinni2; Chang Tingting2; Gao Shuo2; 3; Li Hong2
(1.Jiangsu Second Normal University,Nanjing 210013,China)
(2.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
(3.Nanjing Institute of Environmental Science,Ministry of Ecology and Environment,Nanjing 210042,China)
关键词:
红耳滑龟; 中华草龟; 微塑料; 肠道微生物; 入侵物种
Keywords:
Trachemys scripta elegans; Chinemys reevesii; microplastic; intestinal microbiome; invasive species
分类号:
Q955
DOI:
10.3969/j.issn.1001-4616.2025.01.008
文献标志码:
A
摘要:
全球淡水生态系统均受到不同程度的微塑料污染,微塑料对水生生物的影响受到广泛关注. 通过比较土著种和入侵种对微塑料暴露生理响应的差异,可以评估微塑料对生物的毒性效应,也有助于了解入侵种在逆境胁迫下的适应策略. 本研究以外来入侵种红耳滑龟和土著种中华草龟作为研究对象,评估在龟卵孵化期添加微塑料(1 mg/L)和稚龟生长期添加不同浓度的微塑料(0.1 mg/L、1 mg/L、10 mg/L)对两种龟肠道菌群的影响. 结果表明,在孵化期添加微塑料造成两个物种肠道菌群丰度和多样性下降. 在生长期添加微塑料并没有使两个物种肠道菌群的多样性和丰度出现显著差异,但使组成结构出现显著差异. 在门水平,变形菌门(Proteobacteria)、厚壁杆菌门(Firmicutes)等是两个物种共有的优势菌门; 在属水平,两个物种的优势菌属除杆菌属(Bacteroides)外各有不同,且各种菌所占比例均差异显著. 抵抗不良环境的菌在红耳滑龟体内显著富集,而多种条件致病菌在中华草龟体内显著富集. 研究结果表明,红耳滑龟耐受微塑料胁迫的能力比中华草龟更强.
Abstract:
Global freshwater ecosystems are subject to different degrees of microplastics pollution,and the effects of microplastics on aquatic organisms have received considerable attention. By comparing the differences in physiological responses to microplastic exposure between native and invasive species,the toxic effects of microplastics on organisms can be assessed,and it will also help to understand the adaptive strategies of invasive species under adversity stress. The present study had investigated the impact of microplastics on the gut microbiota of two turtle species including the invasive Trachemys scripta elegans and the native Chinemys reevesii. Microplastics were added to the incubation period(1 mg/L)and the growth period of juvenile turtles(0.1 mg/L,1 mg/L,10 mg/L)in laboratory environment. The results suggested that exposure to microplastics during incubation period resulted in a reduction in the abundance and diversity of gut microbiota in T.scripta elegans and C.reevesii. Exposure to different concentrations of microplastics during the growth period did not cause significant differences in the diversity and abundance of gut microbiota,but caused significant differences in the compositional structure of the gut microbiota. Proteobacteria,Firmicutes were the dominant phyla shared by two species. The dominant genera of the two species were different except for Bacteroides,and the proportions of various bacteria varied significantly. Bacteria resistant to adverse environments were significantly enriched in T.scripta elegans,while a variety of conditionally pathogenic bacteria were significantly enriched in C.reevesii. The results showed that T.scripta elegans was more tolerant to adverse environments than the C.reevesii.

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备注/Memo

备注/Memo:
收稿日期:2023-11-20.
基金项目:国家自然科学基金项目(32171495).
通讯作者:高朔,助理研究员,研究方向:动物生理生态. E-mail:gaoshuo19970419@163.com
更新日期/Last Update: 2025-02-15