[1]薛少帅,王 悦,井木子,等.聚苯乙烯微塑料对小管福寿螺抗氧化酶活性和肠道菌群的影响[J].南京师大学报(自然科学版),2025,48(04):51-57.[doi:10.3969/j.issn.1001-4616.2025.04.005]
　Xue Shaoshuai,Wang Yue,Jing Muzi,et al.Effects of Polystyrene Microplastics on the Antioxidant Enzyme Activity and Intestinal Microbiota of Pomacea canaliculata[J].Journal of Nanjing Normal University(Natural Science Edition),2025,48(04):51-57.[doi:10.3969/j.issn.1001-4616.2025.04.005]
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聚苯乙烯微塑料对小管福寿螺抗氧化酶活性和肠道菌群的影响()

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
48
期数:
2025年04期
页码:
51-57
栏目:
生物学
出版日期:
2025-08-20

文章信息/Info

Title:
Effects of Polystyrene Microplastics on the Antioxidant Enzyme Activity and Intestinal Microbiota of Pomacea canaliculata
文章编号:
1001-4616(2025)04-0051-07
作者:
薛少帅1; 王 悦1; 井木子1; 王聪聪2; 肖溢洁1; 常婷婷2; 沈雯佳1; 李 宏2; 陈 炼1
(1.南京林业大学生命科学学院,江苏 南京 210037)
(2.南京师范大学生命科学学院,江苏 南京 210023)
Author(s):
Xue Shaoshuai1; Wang Yue1; Jing Muzi1; Wang Congcong2; Xiao Yijie1; Chang Tingting2; Shen Wenjia1; Li Hong2; Chen Lian1
(1.College of Life Sciences,Nanjing Forestry University,Nanjing 210037,China)
(2.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)
关键词:
微塑料; 小管福寿螺; 抗氧化酶; 肠道微生物
Keywords:
microplastics; Pomacea canaliculata; antioxidant enzymes; gut microbiome
分类号:
Q985.1
DOI:
10.3969/j.issn.1001-4616.2025.04.005
文献标志码:
A
摘要:
微塑料是一种新型环境污染物,潜在危害水生生物健康. 本实验探究了不同粒径(0.5 μm和5 μm)及浓度(4 mg/L和40 mg/L)的聚苯乙烯颗粒在96 h暴露后对小管福寿螺(Pomacea canaliculata)抗氧化酶活性和肠道菌群的影响. 结果表明,微塑料处理组的小管福寿螺肝胰腺超氧化物歧化酶(SOD)活性显著降低,过氧化氢酶(CAT)活性不存在显著差异,还原型谷胱甘肽(GSH)含量均降低. 不同处理组下小管福寿螺肠道菌群的α多样性没有显著差异; 0.5 μm,4 mg/L组β多样性与其他处理组存在显著差异. 在0.5 μm微塑料的处理下,小管福寿螺肠道厚壁菌门(Firmicutes)相对丰度显著降低,而在40 mg/L两种粒径微塑料处理下乳球菌属(Lactococcus)相对丰度显著降低,并且存在微杆菌属(Microbacterium)、假单胞杆菌属(Pseudomonas)等致病菌的富集. 综上,不同浓度、粒径聚苯乙烯微塑料可诱导小管福寿螺氧化应激反应,虽未对小管福寿螺肠道菌群的多样性产生显著影响,但改变了肠道菌群结构,并导致多种致病菌的富集.
Abstract:
Microplastics,emerging as a novel environmental pollutant,pose potential health risks to aquatic organisms. This study investigated the effects of polystyrene microplastics on antioxidant enzyme activities and gut microbiota in Pomacea canaliculata. Experiments were conducted using polystyrene microplastics partices with diameters of 0.5 μm and 5 μm,at concentrations of 4 mg/L and 40 mg/L,over a period of 96 h. The results showed that the activity of superoxide dismutase(SOD)was significantly reduced in the microplastic treatment groups,while no significant difference was observed in catalase(CAT)activity. Additionally,the content of reduced glutathione(GSH)was decreased in the microplastic treatment groups. The α-diversity of gut microbiota in P.canaliculata exhibited no significant variation across treatment groups. However,β-diversity demonstrated notable differences,particularly between the 0.5 μm(4 mg/L)group and other treatment groups. Exposure to 0.5 μm microplastics at different concentrations(4 mg/L and 40 mg/L)induced a significant reduction in the relative abundance of Firmicutes in the gut. In contrast,exposure to 40 mg/L microplastics of both sizes(0.5 μm and 5 μm)led to a pronounced decrease in Lactococcus levels,with an enrichment of pathogenic bacteria such as Microbacterium and Pseudomonas. In conclusion,although polystyrene microplastics induced an oxidative stress response in P.canaliculata,they did not significantly affect the diversity of its gut microbiota. Nevertheless,these microplastics altered the microbial community structure,promoting the proliferation of various pathogenic bacteria.

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备注/Memo

备注/Memo:
收稿日期:2024-12-17.
基金项目:国家自然科学基金项目(32170434)、江苏省大学生实践创新训练计划项目(202210298150Y).
通讯作者:陈炼,博士,教授,研究方向:入侵生物学. E-mail:chenlian_2004@163.com
更新日期/Last Update: 2025-08-20