[1]曹文杰,赵守祺,高古博,等.共培养体系下融合魏斯氏菌XG-3胞外多糖的产量优化及特性研究[J].南京师大学报(自然科学版),2026,49(02):49-58.[doi:10.3969/j.issn.1001-4616.2026.02.005]
　Cao Wenjie,Zhao Shouqi,Gao Gubo,et al.Optimization of Exopolysaccharide Production by Weissella confusa XG-3 in a Co-culture System and Characterization of its Properties[J].Journal of Nanjing Normal University(Natural Science Edition),2026,49(02):49-58.[doi:10.3969/j.issn.1001-4616.2026.02.005]
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共培养体系下融合魏斯氏菌XG-3胞外多糖的产量优化及特性研究()

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
49
期数:
2026年02期
页码:
49-58
栏目:
生物学
出版日期:
2026-04-10

文章信息/Info

Title:
Optimization of Exopolysaccharide Production by Weissella confusa XG-3 in a Co-culture System and Characterization of its Properties
文章编号:
1001-4616(2026)02-0049-10
作者:
曹文杰; 赵守祺; 高古博; 杨舒涵; 杜仁鹏; 赵 丹
黑龙江大学生命科学学院,农业微生物技术教育部工程研究中心,黑龙江省寒区植物基因与生物发酵重点实验室,黑龙江省普通高校微生物重点实验室,黑龙江 哈尔滨 150080
Author(s):
Cao Wenjie; Zhao Shouqi; Gao Gubo; Yang Shuhan; Du Renpeng; Zhao Dan
School of Life Sciences,Heilongjiang University,Engineering Research Center of Agricultural Microbiology Technology,Ministry of Education,Heilongjiang Provincial Key Laboratory of Plant Genetic Engineering and Biological Fermentation Engineering for Cold Region,Heilongjiang Provincial Key Laboratory of Microbiology,Harbin 150080,China
关键词:
共培养; 融合魏斯氏菌; 胞外多糖; 优化; 性质
Keywords:
co-culture;  Weissella confusa;  exopolysaccharide;  optimization;  properties
分类号:
Q935
DOI:
10.3969/j.issn.1001-4616.2026.02.005
文献标志码:
A
摘要:
本研究建立融合魏斯氏菌(Weissella confusa)XG-3与休哈塔假丝酵母(Candida shehatae)共培养体系,采用单因素法优化菌株XG-3产胞外多糖(exopolysaccharide,EPS),并探究EPS的性质. 结果表明,在W.confusa XG-3单培养4 h后接入C.shehatae,接种比例为1:1,EPS产量达到最大(56.82±0.12)g/L,是单培养的1.63倍. 优化后XG-3产EPS的最优培养条件:蔗糖7%、酵母浸粉0.3%、蛋白胨1.2%、硫酸镁0.03%、柠檬酸铵0.4%、磷酸氢二钾0.3%、吐温-80 0.05%、乙酸钠5%、牛肉膏10%、初始pH 6.0、培养温度30 ℃、摇床转速120 rpm. 优化后菌株W.confusa XG-3的EPS产量为(89.32±0.55)g/L,较优化前提高2.6倍. 共培养XG-3 EPS具有良好的乳化性(对豆油和汽油的乳化率分别为(92.65±0.07)%和(81.08±0.09)%)、特性粘度和重金属吸附能力.
Abstract:
In this study, a co-culture system integrating Weissella confusa XG-3 and Candida shehatae was established. The One-Factor-At-A-Time method was used to optimize exopolysaccharide production by XG3, and the properties of the produced EPS were investigated. The results demonstrated that when C.shehatae was inoculated 4 h after W.confusa XG-3 at an inoculation ratio of 1:1, the EPS yield reached its maximum(56.82±0.12)g/L, which was 1.63 times higher than that obtained from monoculture. The optimal medium formulation for EPS production was composed of 7% sucrose, 0.3% yeast extract, 1.2% peptone, 0.03% magnesium sulfate, 0.4% ammonium citrate, 0.3% dipotassium phosphate, 0.05% Tween-80, 5% sodium acetate, and 10% beef extract. The optimal culture conditions were an initial pH of 6.0, an incubation temperature of 30 ℃, and a shaking speed of 120 rpm. Based on optimization, the EPS yield of the W.confusa XG-3 strain reached(89.32±0.55)g/L, representing a 2.6-fold increase compared to before optimization. The EPS produced under co-culture exhibited excellent emulsifying activity(the emulsification indices for soybean oil and gasoline were(92.65±0.07)% and(81.08±0.09)%, respectively), a high intrinsic viscosity, and strong heavy-metal adsorption capacity.

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备注/Memo

备注/Memo:
收稿日期:2025-12-02.
基金项目:国家自然科学基金项目(32072189)、黑龙江省高校基本科研业务费科研项目(2024-kYYWF-0127).
通讯作者:赵丹,博士,教授,研究方向:微生物次生代谢产物与生理. E-mail:zhaodan4u@163.com
更新日期/Last Update: 2026-04-10