[1]杨筱曼,张伟,黄新华,等.富硒乳酸菌干预肝纤维化大鼠GSH-Px和免疫细胞活性的变化[J].南京师大学报(自然科学版),2007,30(03):93-96.
　Yang Xiaoman,Zhang Wei,Huang Xinhua,et al.Changes of GSH-Px and Immune Cell Activities in Hepatic Fibrosis Rats Intervened by Se-Enriched Lactobacillus[J].Journal of Nanjing Normal University(Natural Science Edition),2007,30(03):93-96.
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富硒乳酸菌干预肝纤维化大鼠GSH-Px和免疫细胞活性的变化()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第30卷
期数:
2007年03期
页码:
93-96
栏目:
生命科学
出版日期:
2007-09-30

文章信息/Info

Title:
Changes of GSH-Px and Immune Cell Activities in Hepatic Fibrosis Rats Intervened by Se-Enriched Lactobacillus
作者:
杨筱曼;张伟;黄新华;梁军清;陈龙;
南京师范大学生命科学学院, 江苏省生物医药功能材料工程研究中心, 江苏南京210097
Author(s):
Yang Xiaoman; Zhang Wei; Huang Xinhua; Liang Junqing; Chen Long
School of Life Science,Nanjing Normal University,Jiangsu Engineering Research Center of Biomedical Function Materials,Nanjing 210046,China
关键词:
富硒乳酸菌;  大鼠;  肝纤维化;  谷胱甘肽过氧化物酶;  免疫细胞
Keywords:
Se-enr iched lactobacillus;  ra t;  hepatic fibrosis;  GSH-Px;  imm uno log ical cell
分类号:
R575.2
摘要:
选用健康SD大鼠36只,雌雄各半,随机分为对照组(C组)、CCl4注射组(CCl4组)和富硒乳酸菌制剂保护组(CCl4-Se组).CCl4组和CCl4-Se组皮下注射CCl4诱发肝纤维化.C组和CCl4组给予普通全价饲料,CCl4-Se组喂含富硒乳酸菌的全价饲料.分别在第4、8周测定肝脏指数,肝组织匀浆谷胱甘肽过氧化物酶(GSH-Px)的活性,血中T淋巴细胞比例,红细胞的免疫功能.结果显示,在整个实验期间,CCl4-Se组的肝脏指数介于对照组和CCl4组之间.CCl4组肝匀浆中GSH-Px活性与C组比较均明显下降,CCl4-Se组的GSH-Px活性虽较C组低,但高于或明显高于CCl4组.CCl4组大鼠T-淋巴细胞(%)、RBC-CR1花环率和RBC-IC花环率都较对照组下降或显著下降,而CCl4-Se组大鼠T-淋巴细胞(%)虽与对照组相比也下降,但明显高于CCl4组,其RBC-CR1花环率和RBC-IC花环率也均高于CCl4组,并与对照组相当.提示:富硒乳酸菌制剂通过保护肝组织抗氧化酶活性,改善和提高免疫细胞功能活动发挥正常有效的作用,从而干预肝纤维化发生发展.
Abstract:
Thirty-six healthy SD ra ts w ith half o f fem a le and m alew ere chosen and random ly d iv ided into a control group( g roup C ), a CCl4 injected g roup ( g roup CC l4 ) and a CC l4 plus Se-enriched lactobacillus g roup ( group CCl4 -Se) . The num ber o f rats in each group is 12. H epatic fibrosis was induced by ca rbon tetrachlor ide ( CC l4 ). G roup CC l4 and g roup CCl4 Se-were given hypoderm ic injection o f olive o il so lution conta in ing 500 g /L CC l4, fo llowed by in jection o f the sam e so lution ( 0. 3mL /100 g body m ass) ev ery 3 days over e ightw eeks. The rats in g roup C received hypoderm ic in jection of o live o il at the sam e dose and frequency. During the entire expe rim en tal pe riod, the comm on com plete d iet w as ora lly prov ided da ily in g roup C and g roup CC l4, and the ra ts in g roup CC l4-Sew ere g iven the diets w ith 3 m g of Se-enr iched lac tobacillus per kg feed. The hepatic index, GSH-Px activ ity in liver hom og enate, T-lym phocy te rates in blood, chang es o f RBC-CR1 rose tte rates and RBC-IC rose tte rates w ere determ ined in the 4 th and 8th w eek po st CC l4 injection. The result show ed that during the whole experim enta l pe riod, the hepatic index, w hich was h ighest in g roup CC l4, was low er o r sign ificantly low er in group CC l4 se- than in g roup CC l4 but notably higher than in g roup C. Compared w ith the g roup C, the GSH-Px activity in liver homogena te in the g roup CC l4 decreased significantly, but in group CCl 4-Se, it w as higher o r sign ificantly higher than tha t in the group CC l4 and was close to that in group C. In group CC l4, T-lym phocy tes(% ) dropped sig n ificantly. T lym phocytes(% ) o f group CCl4 Se- is low er than tha t o f group C, but show ing an apparentincrease as com pared w ith group CC l4. RBC-CR1 rosette ra tes(% ) and RBC-IC rosette ra tes(% ) o f g roup CC l4 w ere low er or significantly lowe r than those of g roup C. H ow ever, bo th rates(% ) of g roup CC l4Se- we re m arked ly low er than those in group CC l4 and c lo se to those in group C. It is sugg ested tha t Se-enriched lactobacillus could intervene the lip id pe rox idation reaction o f hepatic fibrosis induced by CC l4, and ame liorate and enhance imm uno log ic ce ll function activ ity to keep its norm a l and benefic ial ac tion through anti-dam age pro tection

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备注/Memo

备注/Memo:
基金项目: 江苏省重点实验室开放基金( K JS02022 )、江苏省生物医药功能材料工程研究中心资助.
作者简介: 杨筱曼( 1982- ) , 女, 硕士研究生, 主要从事动物免疫生理学与生物化学的学习与研究. E-m ail:elver1982@ hotmail.com
通讯联系人: 陈龙( 1964- ) , 教授, 博士, 主要从事免疫生理学与生物化学的教学与研究. E-m ail:lchen@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05