[1]颜媛,刘吉华,余伯阳,等.赖氨酸6-氨基转移酶合成条件的优化[J].南京师大学报(自然科学版),2008,31(01):75-78.
　Yan Yuan,Liu Jihua,Yu Boyang,et al.Optimization of Lysine 6-aminotransferase Activity by Shake-Flask Fermentation[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(01):75-78.
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赖氨酸6-氨基转移酶合成条件的优化()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年01期
页码:
75-78
栏目:
化学
出版日期:
2008-03-30

文章信息/Info

Title:
Optimization of Lysine 6-aminotransferase Activity by Shake-Flask Fermentation
作者:
颜媛;刘吉华;余伯阳;张剑;
中国药科大学中药复方研究室, 江苏南京210038
Author(s):
Yan Yuan;  Liu Jihua;  Yu Boyang;  Zhang Jian
Department of Complex Prescription of TCM, China Pharmaceutical University, Nanjing 210038, China
关键词:
赖氨酸6-氨基转移酶;  优化;  发酵;  L-哌可酸
Keywords:
Lysine 6- am ino transferase;  optim ization;  ferm en tation;  L-PA
分类号:
TQ464.8
摘要:
以一株从土壤中分离、筛选到轮枝霉菌(Verticillium sp.)2-14为起始菌株,在摇瓶发酵水平上对其产赖氨酸6-氨基转移酶的条件,包括不同碳源、氮源、磷酸盐、表面活性剂等进行了优化,并对基本无机盐离子进行了正交实验分析.结果表明,该菌株产LAT最佳液体发酵培养基为:0·2%L-赖氨酸,3%可溶性淀粉,0·5%KH2PO4,0·1%MgSO4·7H2O.通过考察菌株生长与产酶进程发现,培养52h时酶活达到峰值1217U·L-1,与优化前相比,酶活力提高一倍.
Abstract:
The optima l fermentation cond itions for Lysine 6- am ino transferase( LAT) from Verticillium sp. in shake- flask cu ltivation we re investigated, inc luding carbon source, nitrogen source, phospha te, and surfac tant. Optim a l inorgan ic sa ltw as obta ined by orthogona l exper im ent. The resu lt show ed tha t 0.2% L-Lys, 30% Am idu lin, 0.5% KH 2 PO4, 0.1% M gSO4 7H 2 O could enhance the y ie ld o f LAT. After 52h culture LAT activ ity is one time strong er than before, wh ich reached its peak, 1 217U L- 1.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 中药资源研究开发生物技术平台的构建(江苏省经贸委, 2005)
作者简介: 颜  媛( 1981- ) , 女, 硕士研究生, 研究方向: 中药生物化学技术. E-m ail:yanm oonl it@ 163. com
通讯联系人: 余伯阳( 1969- ) , 教授, 博士生导师, 研究方向: 中药生物化学. E-m ail: boyangyu@ yahoo. com. cn
更新日期/Last Update: 2013-05-05