[1]计汪栋,施国新,徐勤松,等.水鳖叶片矿质营养、保护酶活性及同工酶对Cu~(2+)胁迫的响应[J].南京师大学报(自然科学版),2008,31(01):93-99.
 Ji Wangdong,Shi Guoxin,Xu Qinsong,et al.Effects of Copper Stress on Mineral Nutrient,Protective Enzyme Activities and Their Isozyme Expression in Leaf of Hydrocharis dubia[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(01):93-99.
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水鳖叶片矿质营养、保护酶活性及同工酶对Cu~(2+)胁迫的响应()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年01期
页码:
93-99
栏目:
生命科学
出版日期:
2008-03-30

文章信息/Info

Title:
Effects of Copper Stress on Mineral Nutrient,Protective Enzyme Activities and Their Isozyme Expression in Leaf of Hydrocharis dubia
作者:
计汪栋;施国新;徐勤松;许晔;张慧;赵娟;
南京师范大学生命科学学院, 江苏南京210046
Author(s):
Ji Wangdong Shi Guoxin Xu Qinsong Xu Ye Zhang Hui Zhao Juan
School of Life Science, Nanjing Normal University, Nanjing 210046, China
关键词:
水鳖 Cu 2+胁迫 矿质营养 保护酶 同工酶
Keywords:
H ydro charis dubia Cu 2+ stress m ineral nutrient pro tective enzym e iso zym e
分类号:
Q945
摘要:
通过溶液培养试验研究了不同Cu 2+浓度(0、2、4、6、8mg/L)胁迫下,通过溶液培养试验对浮水植物水鳖叶片矿质营养吸收、活性氧产生、保护酶活性及同工酶的影响.结果表明,(1)随着Cu 2+浓度的增加,Cu 2+对水鳖的矿质营养吸收产生了影响,主要是促进对Ca 2+、Fe 3+、Mg 2+、Zn 2+的吸收,降低对Mn2+、P、K+的吸收.(2)SDS-PAGE蛋白电泳图的条带随Cu 2+浓度增加而逐渐减少,亮度也随之减弱,导致了多肽明显丢失.(3)谷胱甘肽还原酶(GR)活性和过氧化氢(H2O2)含量的变化趋势呈单峰曲线, 在Cu 2++ 浓度为6m g /L时, 均达到最大值, 分别为对照的2.51 倍和3.74 倍. 超氧阴离子( O2 - )和丙二醛(MDA)含量的变化呈先缓慢后讯速上升趋势, 当Cu 2+ 浓度最高时均达顶峰, 分别为对照的342. 3%和334. 2%; 超氧化物歧化酶( SOD) 的活性呈下降趋势, 过氧化物酶( POD)的活性呈上升趋势, 过氧化氢酶( CAT)活性仅略有上升. ( 4)同工酶酶谱分析显示, 随着 Cu 2+ 浓度的增加, SOD谱带数和表达量均减少. POD谱带数由少逐渐增多, 由4带增加到7带, 表达量也呈现上升趋势. CAT 酶谱带数无明显变化, 表达量略存在差异. Cu 2+ 对水鳖的致死浓度范围在2~ 4m g/L.
Abstract:
Hydrocharis dub ia plants exposed to 0, 2, 4, 6, 8m g /L Cu 2+ fo r 7 d w ere ana lyzed w ith re ference to its tox ic im pact on m inera l nutr ient adsorption, active oxygen generation, protec tive enzym e activ ities and the ir iso zym e express ion. The resu lts showed that, w ith the rise of Cu 2+ concentration in culturem edium: ( 1) them ineral nu trien t abso rption w as affected by Cu 2+ stress, as it increasd the abso rption of Ca 2+ 、Fe 3+ 、M g 2+ and Zn 2+ , and reduced tha t o f M n 2+ 、P and K+ , w ith increasing the concentration o f Cu 2+ . ( 2) The am ount and intensity o f prote in / peptide band dec reased gradua lly w ith augm ent of Cu2+ concentration w as obse rved in SDS- PAGE, respectively, and m any po lypeptides d isappeared sign ificantly in fronds treated w ith Cu2+ stress. ( 3) GR activ ity and H2O2 content change tendency assume the sing le peak curv e, in Cu 2+ concentration to 6 m g /L, the ir da tum up to m ax im ize, respec tive ly be tter 2. 51 tim es and 3.74 tim es than the con tro .l The O2 - con tent andMDA content chang e assum e first slow, and then fast trend o f esca la tion, when Cu 2+ concentration to 8m g /L, the ir datum reach ing the c rest, compar ing 342.3% and 334. 2% to the contro;l SOD activ ity assumes the drop tendency, POD ac tiv ity assum es the trend of esca la tion, and CAT activ ity only has sligh tly rise. ( 4) Their isoenzym ic spectrum analysis demonstra tion, along w ith Cu 2+ concentra tion increasing, the SOD isoenzym ic band number and the expression quan tity reduces. The POD isoenzym ic band number gradua lly inc reases, increasing from 4 be lts to 7 be lts, and the expression quantity a lso presen ts the trend o f esca la tion. The CAT isoenzym ic band number does no t obv iously change, and the expression quantity has slight d iffe rence. The conc lusion could be reached that the death of plant resulted from destruction of structure founda tion o f physio log ica l function, unbaance o f ion equilib rium and d iso rder o f physio log ica lm etabo lism. The letha l concentra tion scope of Cu 2+ toHydrocharis dubia was 2~ 4m g /L based on the expe rim en t results

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30370083, 30670121) , 高等学校博士学科点专项科研基金( 20050319005 ), 江苏省教育厅自然科学基金( 05K JB180067 )资助项目.
作者简介: 计汪栋( 1980-) , 硕士研究生, 研究方向: 重金属对水生高等维管束植物的生态效应. E-m ail:jiw angdong@ tom. com
通讯联系人: 施国新( 1950- ) , 教授, 博士生导师, 研究方向: 重金属对水生高等维管束植物的生态效应. E-m ail:gxsh@i n jnu. edu. cn
更新日期/Last Update: 2013-05-05