[1]许冬梅,刘雳,程翼宇,等.橙皮素对血管内皮细胞NO分泌功能的影响[J].南京师大学报(自然科学版),2008,31(03):96-99.
　Xu Dongmei,Liu Li,Cheng Yiyu.Effect of Hesperetin on Release of Nitric Oxide From Endothelial Cells[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(03):96-99.
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橙皮素对血管内皮细胞NO分泌功能的影响()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年03期
页码:
96-99
栏目:
生命科学
出版日期:
2008-09-30

文章信息/Info

Title:
Effect of Hesperetin on Release of Nitric Oxide From Endothelial Cells
作者:
许冬梅;刘雳;程翼宇;
浙江大学药物信息学研究所, 浙江杭州310058
Author(s):
Xu Dongmei; Liu Li; Cheng Yiyu
Pharmaceutical Informatics Institute,Zhejiang University,Hangzhou 310058,China
关键词:
橙皮素;  人脐静脉内皮细胞(HUVECs);  一氧化氮;  雌激素受体
Keywords:
hesperetin;  hum an um bilical ve in endo the lial ce lls ( HUVEC s);  nitr ic ox ide;  estrogen receptor
分类号:
R285
摘要:
考察了橙皮素对人脐静脉内皮细胞( HUVEC s) NO分泌功能的影响, 探讨其作用机制. 为此采用DAN 法测定HUVEC s分泌NO的水平, 用人乳腺癌M CF- 7细胞( 雌激素受体阳性)促增殖实验和报告基因模型实验评价橙皮素的雌激素样活性. 结果显示在内源雌激素水平较低的条件下, 橙皮素在12. 5~ 100 μm o l/L浓度范围内, 能够促进HUVECs分泌NO, 并呈剂量依赖性. 此作用能够被雌激素受体拮抗剂ICI182, 780和放线菌素D阻断. 橙皮素与E2 同时作用时, HUVECs分泌NO水平较两者单独作用时显著下降. 而在内源雌激素水平较高的条件下, 橙皮素抑制HUVECs分泌NO. 橙皮素能够促进M CF- 7细胞增殖, 并且能够被IC I182, 780 完全阻断, 同时, 橙皮素能够部分拮抗E2 对MCF- 7细胞的促增殖作用. 另外, 橙皮素能够诱导ERα控制的报告基因表达. 从而可认为橙皮素属于雌激素受体部分激动剂, 对血管内皮细胞NO 分泌功能具有调节作用, 其机理涉及雌激素受体信号途径和基因转录调节.
Abstract:
Ob jective to investigate the e ffect o f hesperetin on re lease of n itr ic ox ide from cu ltured hum an um bilical ve in endothelial cells ( HUVECs) and poss ible m echan ism. M e thods F luo rom etr ic assay w ith 2, 3-diam inonaphthalene ( DAN) w as em ployed to determ inate the concentration of n itr ite in m ed ium, indica ting NO production from HUVECs. Them odifiedMCF- 7 cell pro liferation assay and the reporter-gene assayw ere used to eva luate the estrogenic activ ity o f hespere tin. Results at re la tive low leve l o f endogenous estrog en, hespe retin ( 12.5~ 100 μmo l/L) prom o ted the release o f NO from HUVECs in a dose-dependent m anner, wh ich cou ld be inhib ited by IC I182, 780 and actinom yc in D. W hen HUVECs w ere treated w ith comb ination o f hesperetin and E2, the leve l of NO re leased from HUVEC sw as low er than that o f sing le treatment. Under h igh level of endogenous estrog en, hesperetin inhib ited the release of NO from HUVECs. H espe re tin sign ifican tly promo ted the pro liferation ofMCF- 7 ce lls, wh ich cou ld be comp le tely b locked by ICI182, 780. M eanwh ile, hespere tin appeared to com prom ise e ffic iency o f E2. A lso hesperetin activa ted estrogen receptor and induced the expression of Lucife rase ( reporter gene). Conclusion hesperetin can be classified as a partia l agonist of estrog en-receptor and possess regulation activ ity on NO production from HUVEC s. The possible m echan ism invo lves in estrogen ic ac tiv ity and regu lation o f gene expression

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备注/Memo

备注/Memo:
基金项目: 国家重点基础研究发展计划项目( 2005CB 523402) .
通讯联系人: 刘 雳, 博士后, 研究方向: 中药与天然药物活性成分筛选和分析. E-m ail:bolt liu@ z ju. edu. cn
更新日期/Last Update: 2013-05-05