[1]李大命,陆建明,杨家新,等.萼花臂尾轮虫(Brachionus calyciflorus)非混交雌体与雄体基因组DNA的RAPD分析[J].南京师大学报(自然科学版),2008,31(03):109-112.
　Li Daming,Lu Jianming,Yang Jiaxin,et al.RAPD Analysis of Genome DNA on the Amictic Female and Male of Brachionus calyciflorus(Rotifera:Monogononta)[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(03):109-112.
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萼花臂尾轮虫(Brachionus calyciflorus)非混交雌体与雄体基因组DNA的RAPD分析()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年03期
页码:
109-112
栏目:
生命科学
出版日期:
2008-09-30

文章信息/Info

Title:
RAPD Analysis of Genome DNA on the Amictic Female and Male of Brachionus calyciflorus(Rotifera:Monogononta)
作者:
李大命;陆建明;杨家新;许晓风;
南京师范大学生命科学学院, 江苏南京210097
Author(s):
Li Daming; Lu Jianming; Yang Jiaxin; Xu Xiaofeng
School of Life Science,Nanjing Normal University,Nanjing 210097,China
关键词:
萼花臂尾轮虫;  RAPD;  非混交雌体;  雄体;  遗传背景
Keywords:
B rachionus calycif lorus;  RAPD;  am ictic fem a le;  ma le;  gene tic background
分类号:
Q953
摘要:
采用RAPD分析技术对萼花臂尾轮虫2种不同性别个体的基因组DNA进行对比分析,探讨萼花臂尾轮虫非混交雌体和雄体性别分化的遗传背景.所用的30条随机引物中,有10条引物在两者基因组中扩增出多态性片段,其中有二者共有条带,也有二者差异条带,显示了萼花臂尾轮虫非混交雌体和雄体遗传背景上的差异.其中引物B15和B18扩增条带具有良好的稳定性和重复性,进一步选取了用此二引物扩增出的雌雄特异性片段各一条进行回收、克隆和测序,测序结果已提交到GenBank数据库,序列号分别为:AY907696和AY898611,同源比较未发现有高同源性的已知序列与之匹配, 表明它们可能是2条新的DNA序列.
Abstract:
In o rde r to acqure the know ledge o f the genetic backgrounds wh ich lead to the sex differentiation o fBrachionus caly cif lorus( Ro tifera), the genom ic DNA of am ictic fem ale and m ale o fB rachionus calycif lorus w as analyzed by RAPD techno logy. One o rm ore spec ia l bands w as g enerated by 10 prim ers of 30 tested pr im ers in am ictic fema le o rm a le rotifer and it showed the genetic backg rounds difference between am ictic fema le and m ale. By repeated testing, spec ial bands can be produced stably by pr im ers B15 and B18 respectively in am ictic fem a le and m a le ro tifer. The spec ia l band w as separated, c loned and sequenced. The sequence resu lts w ere subm itted to GenB ank and the accession num bers w ere: AY907696 and AY898611. W e found there were no any high hom ology sequence m atching to the tw o sequences and it showed that they cou ld be new DNA sequence.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30371093)资助项目.
通讯联系人: 杨家新, 教授, 博士生导师, 研究方向: 轮虫繁殖学研究与水域生态学. E-m ail:yangj iax in@ n jnu. edu. cn
更新日期/Last Update: 2013-05-05