[1]季英华,史文琦,程兆榜,等.灰飞虱体内共生菌Wolbachia的groEL基因克隆及序列分析[J].南京师大学报(自然科学版),2008,31(04):119-123.
 Ji Yinghua,Shi Wenqi,Cheng Zhaobang,et al.Cloning and Sequencing of groEL From Wolbachia Endosymbiont in Laodelphax striatellus Fallén[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(04):119-123.
点击复制

灰飞虱体内共生菌Wolbachia的groEL基因克隆及序列分析()
分享到:

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年04期
页码:
119-123
栏目:
生命科学
出版日期:
2008-12-30

文章信息/Info

Title:
Cloning and Sequencing of groEL From Wolbachia Endosymbiont in Laodelphax striatellus Fallén
作者:
季英华;史文琦;程兆榜;周彤;林玲;范永坚;周益军;
江苏省农业科学院植物保护研究所, 江苏南京210014
Author(s):
Ji YinghuaShi WenqiCheng ZhaobangZhou TongLin LingFan YongjianZhou Yijun
Institute of Plant Protection,Jiangsu Academy of Agricultural Science,Nanjing 210014,China
关键词:
灰飞虱 共生菌 Wolbachia 序列分析 groEL
Keywords:
Laodelphax s tr ia tellus endosymb iont W olbach ia sequence analysis groEL
分类号:
S435.112.3
摘要:
利用特异性引物,首次在国内克隆获得灰飞虱(Laodelphax striatellusFalln)体内共生菌Wolbachia的groEL基因全长序列,其开放阅读框为1659bp,可编码552个氨基酸,GenBank登录号为:EF468716,与已发布的韩国分离物核苷酸一致性达99.9%,为进一步研究其与灰飞虱传播水稻条纹病毒的关系奠定了基础.
Abstract:
G roEL g ene from W olbachia endosym biont in sm all brown p lant-hoppe r( Laodelphax str ia tellus Fa ll?n), w as amp lified by PCR and cloned. The comp le ted nuc leo tide sequence of the genewas determ ined, wh ich w as the first report in China. It has 1 659 bp nucleotides and encodes 552 am ino ac ids. GenBank accession number is EF 468716. Comparison show ed that this gene had 99.9% identities on nuc leo tides level to DQ 356890, an iso late from South Korea. Based on th is sequence, Furthe r resea rches on function of groEL, such as its relation to r ice str ipe v irus transm ission, can be ca rr ied ou t.

参考文献/References:

[ 1] 谢联辉, 魏太云, 林含新, 等. 水稻条纹病毒的分子生物学[ J]. 福建农业大学学报, 2001, 30 ( 3): 269-279.
[ 2] To riyam a S. Rice str ipe v irus: pro totype o f a new group o f v iruses that replicate in plants and insec ts[ J]. M icrob io logical Sciences, 1986, 3( 11): 347-351.
[ 3] 吴爱忠, 赵艳, 曲志才, 等. 水稻条叶枯病毒( RSV)的SP蛋白在介体灰飞虱内的亚细胞定位[ J]. 科学通报, 2001,46( 14) : 1 183-1 186.
[ 4] W e rren JH. B io logy ofW olbach ia [ J]. Annua l Rev iew o f Entom o logy, 1997, 42: 587-609.
[ 5] W e rren JH, Guo L R, W indsor D W. D istr ibu tion o fW olbach ia in neo trop ica l arthropods[ J]. Pro ceedings o f theRoyal Society of London Series B-B io log ica l Sc iences, 1995, 62: 147-204.
[ 6] 邓可京, 杨琰云, 胡成业. 灰飞虱共生菌Wo lbachia 引起的细胞质不亲和性[ J]. 复旦学报: 自然科学版, 1997, 36( 5): 500-506.
[ 7] Akad F, Dotan N, C zosnek H. Trapping of tom ato y ellow leaf cur l v irus and o ther p lant v iruses w ith a groEL hom o logue from
the wh iteflyB em isia tabaci[ J]. Arch ives of V iro logy, 2004, 149( 8): 1 481-1 497.
[ 8] Akad F, Eybishtz A, Ede lbaum D, et a.l M aking a friend from a foe: expressing a groEL g ene from the white fly B em isia tabaci in the phloem o f tom ato plan ts confe rs res istance to tom ato yellow leaf curl v irus[ J]. Arch ives o f V iro logy, 2007, 152( 7): 1 323- 1 339.
[ 9] Doug las A E. Nutritional interactions in insec t-m icrob ia l symb ioses: aph ids and the ir sym b iotic bacter iaBuchnera[ J] . Annual Review o f Entom o logy, 1998, 43: 17-37.
[ 10] Gray SM, Bane rjee N. M echan ism s of arthropod transm ission o f plant and anim a l viruses[ J]. M icrob io logy andM o lecular Biology Rev iew s, 1999, 63( 1): 128-148.
[ 11] H ogenhout S A, V an derW ilk F, VerbeekM, e t a.l Po tato lea fro ll v irus binds to the equator ia l dom a in of the aphid endosymbio tic groEL homo log[ J]. Journal of V irology, 1998, 72 ( 1): 358-365.
[ 12] M or in S, Ghan im M, Ze idanM, e t a .l A g roEL hom o logue from endosymb iotic bacter ia of thew hite flyBem isia tabaci is implicated
in the circu la tive transm iss ion o f tom ato ye llow lea f cur l virus[ J]. V irology, 1999, 256 ( 1): 75-84.
[ 13] 林林, 吴云锋, 崔晓峰. 玉米蚜体内参与传毒的共生菌g roEL基因的克隆和原核表达[ J]. 中国病毒学, 2003, 18( 1): 54-57.
[ 14] 崔晓峰, 吴云锋, 林林, 等. 桃蚜体内与病毒结合的共生菌Buchnera g roEL基因的克隆和序列分析[ J]. 中国病毒学, 2002, 17 ( 1): 65-72.
[ 15] 吴云锋, 崔晓锋, 林林, 等. 禾谷缢管蚜体内的病毒结合蛋白基因的克隆与原核表达[ J]. 微生物学报, 2002, 42( 4): 448-452.
[ 16] 谭周进, 谢丙炎, 肖启明, 等. 烟粉虱内共生菌g roEL基因的原核表达条件研究[ J]. 微生物学通报, 2005, 32( 4): 57-61.

备注/Memo

备注/Memo:
基金项目: 江苏省重大科技攻关( BE 2005301)、国家支撑计划( 2006BAD02A16、2006BAD08A04 )、江苏省自然科学基金( BK2006164 )、农业部行业专项( nyhyzx07 - 051)、国家自然科学基金( 30300230)资助项目.
通讯联系人: 周益军, 研究员, 研究方向: 植物病毒学研究. E-m ail:yjzhou@ jaas. ac. cn
更新日期/Last Update: 2013-05-05