[1]宋向阳,陈牧,毛连山,等.戊糖己糖混合糖发酵生产乙醇的主要影响因素[J].南京师大学报(自然科学版),2009,32(01):115-119.
　Song Xiangyang,Cheng Mu,Mao Lianshan,et al.Main Factors of Influence for Fermentation of Pentose and Hexose Producing Ethanol[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(01):115-119.
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戊糖己糖混合糖发酵生产乙醇的主要影响因素()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第32卷
期数:
2009年01期
页码:
115-119
栏目:
生物学
出版日期:
2009-03-30

文章信息/Info

Title:
Main Factors of Influence for Fermentation of Pentose and Hexose Producing Ethanol
作者:
宋向阳;陈牧;毛连山;云彩琴;勇强;余世袁;
南京林业大学化学工程学院, 林木遗传与生物技术省部共建教育部重点实验室, 江苏南京210037
Author(s):
Song Xiangyang; Cheng Mu; Mao Lianshan; Yun Caiqin; Yong Qiang; Yu Shiyuan
College of Chemical Engineering,Nanjing Forestry University,Key Laboratory of Forest Genetic & Biotechnology,Ministry of Education,Nanjing 210037,China
关键词:
戊糖;  发酵;  乙醇;  影响因素
Keywords:
pentose;  ferm enta tion;  ethano; l facto rs of in fluence
分类号:
TQ223.122
摘要:
以树干毕赤酵母为发酵菌株,高混合糖(木糖、葡萄糖)为发酵底物,确定树干毕赤酵母高糖浓度发酵时所需的条件.研究结果表明,在高糖浓度乙醇发酵中,树干毕赤酵母较为适宜的发酵温度为30℃,发酵24h,残糖浓度和乙醇浓度分别为0.1g/L和32.5g/L.添加硫酸铵1.1g/L+微量元素发酵效果较好,乙醇浓度为33.2g/L.由典型发酵过程中各物质浓度变化曲线可知,酵母优先利用葡萄糖,发酵12h葡萄糖和木糖的还原糖利用率分别为89.3%、10.7%.待12h葡萄糖几乎被消耗完后,对木糖的利用开始占主导地位,乙醇浓度随着糖的不断消耗而逐渐提高. 发酵28h时乙醇浓度最高, 达到33.2 g /L.
Abstract:
Fe rm entation o f g lucose-xy lose m ixture to ethano l w as investigated w ith P ichia stip itis. To de finite the conditions o f high sugars concentration ferm entation through changes o f cu ltivation conditions. The resu lts of study show ed that it w as fit fo r 30℃ o f tem pera ture in h igh sugars concentra tion ferm enta tion. And the concentration of le ft sug ars and eth- ano l we re 0. 1 g /L, 32.5 g /L respective ly for ferm enta tion in 24 h. It w as better fo r adding ( NH4 ) 2 SO4 and trace ele m ents in ferm enta tion. The concentration of e thano lw as 33.2 g /L. G luco sew as first used by yeast from typical curve o f ferm entation. The utilization ra tes of glucose and xy lose were 89.3%, 10.7% respec tive ly. Itw asm a in use o f xy losea fter 12 h. The concentration o f ethano l increased w ith less of sugars. The concen tration o f ethano l reached m ax im um in 28 h of ferm entation wh ich w as 33. 2 g /L.

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备注/Memo

备注/Memo:
基金项目: 863 计划( 2008AA05Z401)、江苏高校自然科学重大基础研究( 06KJA22015 )、江苏省高技术研究计划( BG2005327)资助项目.
通讯联系人: 宋向阳, 副教授, 博士, 研究方向: 生物化工. E-m ail:xiangyangsong@ hotm ail.com
更新日期/Last Update: 2013-04-23