[1]严洁,周建丽,田超,等.疣尾蜥虎线粒体基因组全序列及其基因组成(英文)[J].南京师大学报(自然科学版),2009,32(04):77-82.
 Yan Jie,Zhou Jianli,Tian Chao,et al.Complete Nucleotide Sequence and Gene Organization of the Mitochondrial Genome of Common House Gecko,Hemidactylus Frenatus[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(04):77-82.
点击复制

疣尾蜥虎线粒体基因组全序列及其基因组成(英文)()
分享到:

《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第32卷
期数:
2009年04期
页码:
77-82
栏目:
生命科学
出版日期:
2009-12-30

文章信息/Info

Title:
Complete Nucleotide Sequence and Gene Organization of the Mitochondrial Genome of Common House Gecko,Hemidactylus Frenatus
作者:
严洁;周建丽;田超;周开亚;
江苏省生物多样性和生物技术重点实验室, 南京师范大学生命科学学院, 江苏南京210046
Author(s):
Yan JieZhou JianliTian ChaoZhou Kaiya
Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
疣尾蜥虎 壁虎科 壁虎类 线粒体基因组
Keywords:
H em idacty lus frenatus Gekkonidae Gekko ta m itochondr ia l genom e
分类号:
Q953
摘要:
测定了疣尾蜥虎(H em idacty lus frenatus ) 的线粒体基因组全序列. 序列全长为16 891 bp, 包括13 个蛋白质编码基因、2 个rRNA 基因和22个tRNA 基因. 除大部分基因由重链编码外, 仅1个蛋白编码基因和8个tRNA 基因由轻链编码. 碱基组成的偏好与其他脊椎动物线粒体DNA接近. 没有发现长的基因间隔区, 说明该基因组的结构十分紧凑. 基因组的组成与典型脊椎动物的相近, 即没有发现重排、基因或控制区的重复等在其它有鳞类动物中出现过的异常特征. 除单性生殖的壁虎外, 现有的壁虎类线粒体基因组在基因含量和顺序上是一致的. 作为蜥虎属线粒体基因组全序列的惟一代表, 该序列有望在有鳞类系统发生的推断上发挥一定的作用.
Abstract:
W e de term ined the com plete m itochondr ia l sequence of the comm on house gecko, H em idactylus frenatus, by us ing the polym erase chain reactions ( PCRs) . The entire m tDNA sequence is 16 891 bp in length and conta ins 13 prote in-coding genes, 2 rRNA genes, 22 tRNAs genes and the noncoding control reg ion. M ost genes are encoded by theH strand, ex cept for one pro te in- cod ing and eight tRNA g enes. The base composition is skew ed sim ilar to o ther vertebra te m tDNAs. No long intergen ic spacers a re found ind ica ting a com pac t com po sition of this genom e. In g ene organ ization, them itochondr ia l genom e ofH. frenatus is identica l to that o f typ ica l verteb rate, that is to say threem a in a typ ica l features ( rearrangem ents, dup lication of genes and of the control reg ion) found in som e squam ates are not found in th is genome. Except the parthenogenetic geckos, known geckos have consistent g ene conten ts and orders. The new comp le te sequence can be useful to im prove the future phy log eny by broaden ing the cov erage o f squam ate d iversity.

参考文献/References:

[ 1] Dong S, Kum azawa Y. Com plete m itochondrial DNA sequences o f six snakes: phy log enetic re lationships and m olecu lar evolution
of genom ic features[ J]. Journa l ofM o lecu lar Evo lution, 2005, 61( 1): 12-22.
[ 2] Yan J, LiH, Zhou K. Evo lution of them itochondrial genom e in snakes: Gene rearrangem ents and phy logenetic re la tionsh ips[ J]. BMC Genom ics, 2008, 9( 1): 569.
[ 3] M acey J R, Papenfuss T J, Kuehl JV, et a.l Phy log enetic re la tionsh ips am ong am ph isbaenian reptiles based on comp letem -i
tochondr ia l genom ic sequences[ J]. M o lecular Phy logene tics and Evolution, 2004, 33( 1) : 22-31.
[ 4] M acey J R, Schu lte J A, Fong J J, et a.l The comp le tem itochondria l genom e of an ag am id lizard from theA fro-Asian subfamily agam inae and the phy logene tic po sition o f Bufonicep s and X enagama [ J]. M o lecular Phy logenetics and Evo lution,
2006, 39( 3) : 881-886.
[ 5] M acey J R, Kuehl JV, La rson A, et a.l Soco tra Island the forgo tten fragm ent of Gondw ana: unm ask ing cham eleon lizard history w ith com plete m itochondr ial genom ic da ta[ J] . M o lecular Phy logenetics and Evo lution, 2008, 49( 3): 1 015-1 018.
[ 6] Kum azawa Y. M ito chondrial DNA sequences of five squam ates: phy logene tic affiliation o f snakes[ J]. DNA Resea rch, 2004,11( 2) : 137-144.
[ 7] Zhou K, L iH, H an D, et a .l The com plete m itochondria l g enom e o fG ekko gecko ( Reptilia: Gekkonidae) and suppo rt fo r them onophy ly o f Sauria inc lud ing Am ph isbaen ia[ J]. M o lecular Phy logene tics and Evolution, 2006, 40( 3) : 887-892.
[ 8] M acey J R, Fong J J, Kueh l J V, et a.l The com plete m ito chondrial genome o f a g ecko and the phy logene tic pos ition o f the M idd le Eastern Teratoscincus key serling i[J]. M o lecu la r Phy log enetics and Evo lution, 2005, 36( 1): 188-193.
[ 9] Bohm eM U, Fritzsch G, T ippm ann A, et a .l The com plete m itochondria l genom e o f the g reen lizard Lacer ta vir idis virid is
( Reptilia: Lace rtidae) and its phy logenetic position w ith in squam ate reptiles[ J]. Gene, 2007, 394( 1-2): 69-77.
[ 10] Townsend T M, Larson A, Lou is E, et a .l M o lecular phylogenetics o f Squam ata: the po sition of snakes, am phisbaen ians,and d ibam ids, and the root o f the squam ate tree[ J]. System atic B io logy, 2004, 53( 5) : 735-757.
[ 11] Page R D M, H o lm es E C. M o lecular Evolution, a Phy logene ticApproach[M ]. Ox fo rd: B lackwe ll Sc ience, 1998: 1-352.
[ 12] Boo re J L. An im al m itochondr ia l genom es[ J] . Nucle ic Ac ids Research, 1999, 27( 8) : 1 767-1 780.
[ 13] Boore J L, B rownW M. B ig trees from little genom es: m ito chondrial g ene o rder as a phylogenetic too l[ J]. CurrentOp in ion in Genetics and Deve lopm en t, 1998, 8( 6): 668-674.
[ 14] M acey J R, Schulte JA, Larson A. Evo lution and phy logene tic inform a tion content o fm itochondr ia l genom ic structural features illustrated w ith acrodont liza rds[ J]. System atic B iology, 2000, 49( 2): 257-277.
[ 15] Am er S A, Kum azaw aY. M itochondria l genom e ofP ogona v itticep es ( Reptilia; Agam idae): contro l reg ion dup lication and the or ig in o f Austra lasian agam ids[ J] . Gene, 2005, 346: 249-256.
[ 16] C arranza S, Arno ld E N. Systema tics, biog eog raphy, and evo lution ofH em idacty lus g eckos ( Reptilia: Gekkon idae ) e luc-idated using m itochondr ia l DNA sequences[ J]. M olecu lar Phylogenetics and Evo lution, 2006, 38( 2): 531-545.
[ 17] M a thews D H, D isneyM D, Childs J L, et a.l Incorporating chem ical mod ification constraints into a dynam ic prog ramm ing a lgo rithm fo r pred iction of RNA secondary structure [ J]. Pro ceedings of the Na tiona l A cadem y of Sc iences, 2004, 101( 19) : 7 287-7 292.
[ 18] TamuraK, Dud ley J, Ne iM, et a.l MEGA4: M o lecu lar Evo lutionary Genetics Ana lys is (M EGA ) softw are version 4. 0[ J].
M o lecu lar B io logy and Evo lution, 2007, 24( 8): 1 596-1 599.
[ 19] Kum azawa Y. M itochondrial g enom es from m a jo r lizard fam ilies suggest the ir phy log enetic re lationsh ips and anc ient rad iations[J]. Gene, 2007, 388( 1-2) : 19-26.
[ 20] W o lstenho lm eD R. Anim a lm ito chondrial DNA: structure and evo lution[ J] . Internationa lRev iew o f Cyto logy, 1992, 141:173-216.
[ 21] Ohtsuk i T, Kawa i G, W atanabeK. Them in im al tRNA: un ique structure o fAscar is suum m itochondr ia l tRNA ( Se r) ( UCU)hav ing a short T arm and lacking the entire D arm [ J] . FEBS Le tters, 2002, 514( 1): 37-43.
[ 22] O jala D, M ontoya J, Attard iG. tRNA punctuation m ode l of RNA processing in hum an m itochondria[ J]. Nature, 1981,290( 5806): 470-474.
[ 23] C layton D A. Replication and transcr iption of ve rtebrate m itochondr ia l DNA [ J]. Annua l Rev iew o f Ce ll B io logy, 1991, 7( 1): 453-478.
[ 24] Shade l G S, C lay ton D A. M ito chondrial DNA m ain tenance in ve rtebrates[ J]. Annual Rev iew of B iochem istry, 1997, 66( 1): 409-435.
[ 25] H ixson J E, W ong T W, C layton D A. Bo th the conserved stem- loop and divergent 5-flanking sequences a re requ ired fo r in itiation at the hum an m ito chondrial or ig in of light- strand DNA replication[ J]. Journal of B io log ica l Chem istry, 1986, 261( 5): 2 384-2 390.
[ 26] Fu jitaM K, Boore J L, Mo ritz C. M ultiple o rig ins and rap id evo lution of duplica ted m itochondr ia l genes in parthenog enetic geckos (H eteronotia binoei; Squam ata, Gekkon idae) [ J]. M o lecu lar B io logy and Evo lution, 2007, 24( 12): 2 775-2 786.
[ 27] Kum azawa Y, OtaH, N ish idaM, et a.l Gene rearrangem ents in snakem itochondr ia l genom es: highly concerted evo lution o f contro-l reg ion- like sequences duplica ted and inserted into a tRNA gene c luster[ J]. M o lecu lar B io logy and Evo lution,1996, 13( 9): 1 242-1 254.
[ 28] Kum azaw a Y, Endo H. M itochondr ia l genom e of the Kom odo dragon: e fficient sequenc ing m ethod w ith reptile-or iented pr ime rs and novel gene rearrangem ents[J] . DNA Research, 2004, 11( 2): 115-125.
[ 29] V ida lN, H edges S B. The phy logeny o f squam ate reptiles ( lizards, snakes, and am phisbaen ians) inferred from n ine nuc lear pro te in-cod ing genes[ J]. Comptes Rendus B io log ies, 2005, 328( 10 /11): 1 000-1 008.
[ 30] V ida lN, H edges S B. The mo lecu lar evolutionary tree of lizards, snakes, and am phisbaen ians[ J]. Com ptes Rendus B io logies, 2009, 332( 2 /3): 129-139.
[ 31] A lbert EM, SanM auroD, Ga rc ia-ParisM, et a.l E ffect of taxon sam pling on recovering the phy logeny o f squam ate reptiles based on com plete m itochondr ia l genom e and nuc lear gene sequence data[ J]. Gene, 2009, 441( 1 /2): 12-21.

备注/Memo

备注/Memo:
Founda tion item: Supported by th eNat ionalNatu ralS cience Foundation ofCh ina ( 30870286) , Nan jing NormalUn iversity Innovative Team Project.
Correspond ing author: Zhou Ka iya, p rofessor, m ajored in zoo logy, E-m ail: kyzhoun@j 126. com
更新日期/Last Update: 2013-04-23