[1]周峰,华春,周泉澄,等.高温胁迫对菠菜类囊体膜蛋白亚基和光谱特征的影响[J].南京师大学报(自然科学版),2010,33(01):98-101.
　Zhou Feng,Hua Chun,Zhou Quancheng,et al.Effect of High Temperature Stress on Protein Subunit and Spectra Characteristic of Thylakoid of Spinach[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(01):98-101.
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高温胁迫对菠菜类囊体膜蛋白亚基和光谱特征的影响()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年01期
页码:
98-101
栏目:
生命科学
出版日期:
2010-03-20

文章信息/Info

Title:
Effect of High Temperature Stress on Protein Subunit and Spectra Characteristic of Thylakoid of Spinach
作者:
周峰1;  华春1;  周泉澄1;  王仁雷2
1. 南京晓庄学院生物化工与环境工程学院, 江苏南京211171 2. 江苏食品职业技术学院, 江苏淮安223003
Author(s):
Zhou Feng1; Hua Chun1; Zhou Quancheng1; Wang Renlei2
1.School of Biochemical and Environmental Engineering,Nanjing Xiaozhuang University,Nanjing 211171,China 2. J iangsu Food Science Co llege, Hua ian 223003, Ch in a
关键词:
类囊体膜;  高温;  光谱
Keywords:
thy lako id;  high tem perature;  reconstitution
分类号:
S636.1
摘要:
研究了高温处理对菠菜类囊体膜蛋白亚基和光谱特征的影响.结果显示,高温处理后,内周天线CP43、放氧外周蛋白33 000降解明显,而外周天线LHC II发生聚集;随着处理温度的增高,菠菜类囊体膜的吸收光谱和荧光发射光谱明显下降,而且峰位发生蓝移,这说明高温处理破坏了类囊体膜的结构,影响到对光能的吸收.而35℃高温处理下,随着处理时间的延长,吸收光谱和荧光光谱也呈现下降趋势,但与不同温度处理相比,下降程度略缓,这说明类囊体膜在35℃长时间处理表现出的耐受性比在高温短时间处理条件强.
Abstract:
The e ffect o f h igh tem pera ture on prote in subun it and spectra charac teristic of thy lako id o f spinach w as studied in this paper. The resu lt show ed that CP43 subunit, ex trins ic oxygen evo lution subunit 33 000 degraded ma rked ly but LHCII agg rega ted under high tem perature treatm ent. The abso rption spectra and fluorescence spectra decreased signifi  cantly, and the peak po sition b lue shifted. It dem onstrated tha t the structure of thy lako id and light abso rption we re de  stroyed w ith increase of tem pe rature. The abso rption spectra and fluorescence spectra also decreased under 35℃ trea t  m ent w ith pro longation o f treating tim e. In contrast, the decrease extent o f absorption spectra and fluo rescence spectra w as sm a ller than that o f under different high temperature treatm ent. It ind icated that the to lerance ab ility o f thy lako id un  der long tim e treatm ent a t 35℃ w as h igher than that o f under sho rt tim e trea tm en t at h igh tem pera ture.

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备注/Memo

备注/Memo:
基金项目: 江苏省高校自然科学基础研究面上项目( 07KJD180126和05KJD180118 )、南京晓庄学院生态学重点学科项目. 通讯联系人: 华春, 女, 教授, 研究方向: 植物生理学. Email: hc3501988@ 163. com
更新日期/Last Update: 2013-04-08