[1]裴凌鹏,尹　霞,崔　箭,等.刺老苞根皮黄酮对H2O2诱导的MC3T3-E1成骨细胞损伤改善[J].南京师大学报(自然科学版),2010,33(01):107-111.
　Pei Lingpeng,Yin Xia,Cui Jian,et al.Improvement of H2O2 Induced MC3T3-E1 Osteoblast Damage by Aralia echinocauIis Hand. Mazz Flavonoids[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(01):107-111.
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刺老苞根皮黄酮对H2O2诱导的MC3T3-E1成骨细胞损伤改善()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年01期
页码:
107-111
栏目:
生命科学
出版日期:
2010-03-20

文章信息/Info

Title:
Improvement of H2O2 Induced MC3T3-E1 Osteoblast Damage by Aralia echinocauIis Hand. Mazz Flavonoids
作者:
裴凌鹏1 ; 尹　霞2 ;  崔　箭1 ;  李　莉2 ;  朱　嘉2
1. 中央民族大学中国少数民族传统医学研究院,北京100081   2. 中国中医科学院望京医院骨伤科研究所,北京100070
Author(s):
Pei Lingpeng1; Yin Xia2; Cui Jian1; Li Li2; Zhu Jia2
1.Central MINZU University,Institute of Minarity Traditional Medicine Research,Beijing 100081,China  2. China Academy of ChineseMedical Sciences, Wangjing Hosp ital, Institute of Orthopaedics and Traumatology, Beijing 100070, China
关键词:
MC3T32E1成骨细胞; 自由基; 刺老苞根皮; 黄酮类化合物
Keywords:
MC3T32E1 osteoblast;  free radical;  A ralia echinocauIis Hand. Mazz;  flavonoids
分类号:
R285
摘要:
在H2O2 胁迫下,研究刺老苞根皮(Aralia echinocau Iis)黄酮增强MC3T32E1成骨细胞抗自由基损伤的作用. 为此用H2O2 作用MC3T32E1成骨细胞,建立自由基损伤细胞模型. 培养的成骨细胞分为对照组、模型组、刺老苞根皮黄酮低剂量组(1 ×10 - 8 mol/L) 、刺老苞根皮黄酮中剂量组(1 ×10 - 7 mol/L)和刺老苞根皮黄酮高剂量组(1 ×10 - 6 mol/L). 测定不同处理组细胞活力、超氧化物歧化酶( superoxide dismutase, SOD)活性、活性自由氧 ( reactive oxygen species, ROS)含量、脂质过氧化物( lip id oxygen, LPO)含量,同时利用荧光偏振法测定细胞膜流动性. 结果显示与模型组相比,刺老苞根皮黄酮组细胞活力、SOD活性、细胞膜流动性均显著升高( P < 0.01) ,而 ROS含量、LPO含量则显著降低( P < 0.01) ,并呈现一定的量效关系. 可以认为H2O2 摄入会导致MC3T32E1成骨细胞的氧化损伤,而刺老苞根皮黄酮可以预防或降低此类损伤对细胞的影响.
Abstract:
Objective to investigate the effect ofA ralia echinocauIis Hand. Mazz flavonoids on enhances the function of an2 ti2oxidative damage inMC3T32E1 osteoblast. MethodsMC3T32E1 osteoblasts were randomly divided in five group s, in2 cluding control group,model group, A ralia echinocauIis Hand. Mazz flavonoids group [ low dose (1 ×10 - 8 mol/L) 、mid2 dle dose (1 ×10 - 7 mol/L ) 、high dose ( 1 ×10 - 6 mol/L ) ] , whose activity of cells、superoxide dismutase ( SOD) 、the content of reactive oxygen species (ROS) 、lip id oxygen (LPO) and membrane fluidity were tested and compared. Re2 sults compared with model group, the activity of cells、SOD activity and membrane fluidity in A ralia echinocauIis Hand. Mazz flavonoids group swere significantly raised ( P < 0.01). However, the content of ROS and LPO were significantly decreased ( P < 0.01). Conclusion H2O2 can cause oxidative damage ofMC3T32E1 osteoblasts, but A ralia echinocauIis Hand. Mazz flavonoids can p revent and decrease its influence.

参考文献/References:

[ 1 ] 　邵兴,赵敬华. 浅析自然地理环境与土家族医药学的关系[ J ]. 中国民族民间医药, 2007, 11 (3) : 58-60.
[ 2 ] 　方志先,赵晖,赵敬华. 土家族药物志[M ]. 北京:中国医药科技出版社, 2007: 830-835.
[ 3 ] 　Kim L. Lycopene II2effect on osteoblasts: the carotenoid lycopene stimulates cell p roliferation and alkaline phosphatase activi2 ty of SaOS22 cells[ J ]. J Med Food, 2003, 6 (2) : 79-86.
[ 4 ] 　Jacobson A. Vitamin A differentially regulates RANKL and OPG exp ression in human osteoblasts[ J ]. Biochem Biophys Res Commun, 2004, 322 (1) : 162-167.
[ 5 ] 　Reiter R. Biomarkers of free radical damage: app lications in experimental animals and in human[ J ]. J Pineal Rea, 1995, 18: 1-11.
[ 6 ] 　Chan T Y. Oxidative DNA damage, antioxidants and cancer[ J ]. J Pineal Rea, 1996, 20: 187-191.

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备注/Memo

备注/Memo:
基金项目:教育部“长江学者与创新团队”计划( Irt0871) 、国家自然科学基金(30902011) . 通讯联系人:裴凌鹏,博士,讲师,研究方向:民族医药与临床学. E-mail: lppei@hotmail. com
更新日期/Last Update: 2013-04-08