[1]杨大朋,王海啸,彭延延,等.蛇床子素对人乳腺癌细胞增殖、细胞周期及凋亡的影响(英文)[J].南京师大学报(自然科学版),2010,33(02):76-80.
　Yang Dapeng,Wang Haixiao,Peng Yanyan,et al.Effects of Osthole on the Proliferation,Cell Cycle and Apoptosis in Human Breast Cancer Cells[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(02):76-80.
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蛇床子素对人乳腺癌细胞增殖、细胞周期及凋亡的影响(英文)()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年02期
页码:
76-80
栏目:
生命科学
出版日期:
2010-06-20

文章信息/Info

Title:
Effects of Osthole on the Proliferation,Cell Cycle and Apoptosis in Human Breast Cancer Cells
作者:
杨大朋1 ;  王海啸1 ;  彭延延1 ;  唐庆九2 ;  马长艳1
1. 南京医科大学发育遗传学系, 江苏南京210029 2. 上海农业科学研究院食用菌研究所, 上海201106
Author(s):
Yang Dapeng1; Wang Haixiao1; Peng Yanyan1; Tang Qingjiu2; Ma Changyan1
1.Department of Developmental Genetics,Nanjing Medical University,Nanjing 210029,China 2. Institute of Ed ib le Fung,i Shangha iAcad emy ofAgricu ltu ral Sciences, Shanghai 201106, China
关键词:
蛇床子素;  乳腺癌;  增殖;  细胞周期;  凋亡
Keywords:
Osthole;  breast cancer;  pro liferation;  cell cycle;  apoptosis.
分类号:
R285;R737.9
摘要:
蛇床子素是从传统的中药蛇床子的果实中提取的香豆素类衍生物.在发达国家以及发展中国家,乳腺癌是发病率和致死率较高的女性肿瘤之一.本研究的目的是调查蛇床子素对人乳腺癌细胞的增殖、细胞周期以及凋亡的影响.蛇床子素的抗细胞增殖活性用MTT法测定.细胞周期的分布以及细胞凋亡用流式细胞术测定.本研究结果表明,蛇床子素对抑制乳腺癌细胞的增殖、促进G1期阻滞以及诱导细胞凋亡有明显作用.该结果提示有必要进一步研究和评估蛇床子素在乳腺癌治疗中的作用.
Abstract:
Ostho le, a na tura l coum arin der iva tive, is ex tracted from the fru it o fCnid ium monn ieri ( L. ) Cusson, a therapeutic agen t in trad itional Chinese med ic ine. B reast cancer is one of them ost common ly diagnosed cancers and the leading cause of death in wom en, in deve loped and increasing ly in deve loping countr ies. The ob jective of th is study is to investiga te the effec ts of Ostho le on the proliferation, ce ll cy cle and apoptosis in hum an breast cance r ce lls. Antipro liferative activ ity o f Ostho le w as m easured w ith the 3-( 4, 5-Dim ethylth iazo-l 2-y l)-2, 5-d ipheny ltetrazo lium brom ide (MTT ) assay. Flow cytom etry was perfo rmed to observe ce ll cycle distribution and apoptotic body appearance. Our resu lts show tha tO sthole is e ffective in inh ibiting cell pro life ra tion, prom otingG1 phase arrest and induc ing apoptosis in breast cancer ce lls. These findings ind icate a need for furthe r eva luating O stho le as an antitumo r agent to trea t breast cancer patients.

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备注/Memo

备注/Memo:
Foundation item: National Natural Science Foundation of China(30500585);the Natural Science Foundation of Jiangsu Province(BK2008450) Corresponding autho r: M a Changyan, associate p rofessor, majored in molecu lar b iology of tum or. E-mail:cyma@njmu.edu
更新日期/Last Update: 2013-04-08