[1]黄慧颖,宋杰,尚广东,等.大肠杆菌链霉菌假单胞菌穿梭表达BAC载体的构建[J].南京师大学报(自然科学版),2010,33(02):104-108.
　Huang Huiying,Song Jie,Shang Guangdong.Construction of an E.coli-Streptomyces-Pseudomonas Shuttle Expression BAC Vector[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(02):104-108.
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大肠杆菌链霉菌假单胞菌穿梭表达BAC载体的构建()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年02期
页码:
104-108
栏目:
生命科学
出版日期:
2010-06-20

文章信息/Info

Title:
Construction of an E.coli-Streptomyces-Pseudomonas Shuttle Expression BAC Vector
作者:
黄慧颖;宋杰;尚广东;
南京市微生物工程技术研究中心, 江苏省生物多样性与生物技术重点实验室, 南京师范大学生命科学学院, 江苏南京210046
Author(s):
Huang Huiying; Song Jie; Shang Guangdong
Nanjing Engineering and Technology Research Center for Microbiology,Jiangsu Key Lab for Biodiversity and Biotechnology,School of Life Science,Nanjing Normal University,Nanjing 210046,China
关键词:
BAC载体;  重组工程;  穿梭表达
Keywords:
BAC vector;  recomb inee ring;  shuttle expression
分类号:
Q78
摘要:
异源表达生理活性物质的生物合成基因簇是药物研发领域的一个重要的方向,它可通过异源表达来研究基因功能,获得目标化合物或对现有的化合物进行结构改造.生物合成基因簇一般较大,常规的DNA载体由于容量不足、拷贝数较低或不能在不同的异源宿主间穿梭表达而难以对其进行操作.本研究运用重组工程策略和常规的克隆手段,将多个异源表达所必须的功能基因克隆至常用的构建BAC文库的载体pECBAC1,获得了一个可在大肠杆菌链霉菌假单胞菌3个常见的异源表达宿主间进行穿梭表达的BAC载体.
Abstract:
H etero logous express ion o f biosynthetic gene c luster is an im po rtant study area o f drug resea rch and development, and it can be used fo r gene function study, to obta in targe t compound o r its de riv atives. A s b iosynthetic gene clus  ter is usua lly very large, no rma l DNA vectors m ay have the prob lem s o f low content, hard to handle because of vector s low copy number or no t su it for shuttle express ion in diffe rent hete ro logous stra ins. In th is wo rk, recom bineer ing strategy as we ll as no rm al gene c lon ing techn iques are used to clone functional hetero logous expression DNA elem ents into pECBAC1, one o f them ost commonly used BAC library construc tion vector.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: “重大新药创制”科技重大专项课题(2009ZX09103-674) 通讯联系人: 尚广东, 副教授, 研究方向: 微生物药物的研发. Email:shanggd@ hotmail. com
更新日期/Last Update: 2013-04-08