[1]张代臻,丁鸽,等.渤海湾口虾蛄线粒体COI基因的遗传多样性研究[J].南京师大学报(自然科学版),2010,33(04):80-83.
　Zhang Daizhen,Ding Ge,Zhang Huabin,et al.Genetic Diversity of Oratosquilla oratoria From Different Sea Area of Bohai Bay by Mitchondrial COI Gene[J].Journal of Nanjing Normal University(Natural Science Edition),2010,33(04):80-83.
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渤海湾口虾蛄线粒体COI基因的遗传多样性研究()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第33卷
期数:
2010年04期
页码:
80-83
栏目:
生命科学
出版日期:
2010-12-20

文章信息/Info

Title:
Genetic Diversity of Oratosquilla oratoria From Different Sea Area of Bohai Bay by Mitchondrial COI Gene
作者:
张代臻1;  丁鸽1;  2;  张华彬1;  唐伯平1
1. 江苏省滩涂生物资源与环境保护重点建设实验室, 盐城师范学院, 江苏盐城224051 2. 盐城工学院化学与生物工程学院, 江苏盐城224003
Author(s):
Zhang Daizhen1; Ding Ge1; 2; Zhang Huabin1; Tang Boping1
1.Jiangsu Provincial Key Laboratory of Coastal Wetland Bioresources and Environmental Protection,Yancheng Normal University,Yancheng 224051,China 2. Chem ical and B iologicalEng ineering Col lege, Yan ch eng In stitute ofT echnology, Yanch eng 224003, Ch ina
关键词:
口虾蛄;  COI基因;  遗传多样性
Keywords:
Oratosqu illa orator ia;  COI gene;  Genetic d iversity
分类号:
S917.4
摘要:
口虾蛄(Oratosquilla oratoria)是我国沿海重要的渔业种,本文对渤海湾口虾蛄COI基因的遗传多样性进行研究,在48条633 bp的COI序列中,存在19个变异位点,且转换高于颠换;不同水域间的平均遗传距离为0.004 9,单元型HP3、HP5、HP14是渤海湾不同水域的共享单元型,且HP5和HP17是渤海湾较为原始的类型,并且歧点分布分析显示渤海湾口虾蛄种群经历过快速扩张过程.本研究将为全面研究渤海湾口虾蛄的遗传多样性、评估野生种质资源情况等积累基本数据.
Abstract:
Ora to squilla oratoria ( Crustacea: Squ illidae) is the m ost dom inant spec ies o f Boha i Bay. In the study, the genetic d ive rsity of O. orator ia from Bohai Bay was stud ied by 48 m itchondria l COI sequences. 19 variab le sites were defined in 633 sites o f the aligned sequences. And the num ber o f transitions w as m ore than transversions. The av erage genetic d istance among sea areas was 0?? 004 9, and HP3、HP5 and H P14 we re shared by d ifferent sea areas of Bohai Bay. H P5 and HP17 were the ancestra l haptypes o f Boha i Bay by parsimony netwo rk. Resu lt o f m ism atch distr ibution im plied rap id expansion o f Boha i popu la tion. Genetic divers ity ana lysis o f COI sequencesw illbe usab le for further asses?? s ing the genetic structure o fO. oratoria.

参考文献/References:

[ 1]堵南山. 甲壳动物学[M ]. 北京: 科学出版社, 1993.
[ 2]黄宗国. 中国海洋生物种类与分布[M ]. 北京: 海洋出版社, 2008.
[ 3] Ke ita K, Takash iY, Takam ich i S, et a.l Age estim ation o f the w ild popu lation of Japanese m antis shrim p O ra to squ illa orato ria ( C rustacea: S tom atopoda) in Tokyo Bay, Japan, using lipo fuscin as an age m arker[ J] . Fisheries Sc ience, 2005, 71: 141-150.
[ 4]林月娇, 刘海映, 徐海龙, 等. 大连近海口虾蛄形态参数关系的研究[ J]. 大连水产学院学报, 2008, 23( 3): 215-217.
[ 5]王春琳, 徐善良, 梅文骧, 等. 口虾蛄的生物学基本特征[ J]. 浙江海洋学院学报, 1996, 15( 1): 60-62.
[ 6]路心平, 马凌波, 乔振国, 等. 利用线粒体DNA 标记分析中国东南沿海拟穴青蟹种群遗传结构[ J]. 水产学报, 2009, 33( 1): 15-23.
[ 7]麦维军, 谢珍玉, 张吕平, 等. 中国明对虾与5种虾类线粒体16S rRNA和COI基因片段的序列比较及其系统学初步研究[ J] . 海南大学学报: 自然科学版, 2009, 27( 1): 15-23.
[ 8]刘明月, 蒋琦辰, 杨家新. 不同海域中国花鲈的细胞色素b序列的遗传分析[ J]. 南京师大学报: 自然科学版, 2010, 33( 1): 102-106.
[ 9]戴建华, 殷文莉, 彭奎东. 青鱼线粒体DNA 限制性酶切图谱的研究[ J]. 南京师大学报: 自然科学版, 2002, 25( 4): 100-104.
[ 10]Sambrook J, Fr itsch E F, M aniatisT. Mo lecu la rC lon ing: A Labo ra to ryM anua l[M ]. 2nd ed. New Yo rk: Co ld SpringHar bor Labo rato ry Press, 1989.
[ 11]Kuma r S, Tamura K, Ne iM. MEGA3: Integ rated softw are for mo lecu lar evolutionary gene tics analysis and sequence alignment[ J]. Br ie fing s in B io inform atics, 2004, 5: 150-163
[ 12]Tem pleton A R, C randa ll K A, S ing C F. A cladistic ana ly sis of phenotypic associations w ith haplotypes in fe rred from re striction endonucleasem app ing and DNA sequence data. III. C ladogram estim ation[ J]. Genetics, 1992, 132: 619-633.
[ 13]C lem en tM, Po sada D, C randa ll K A. TCS: a com puter program to estim ate g ene g enealog ies[ J]. Mo lecu lar E co logy, 2000, 9: 1 657-1 659.
[ 14]Exco ffie r L, Lava lG, Schne ider S. Ar lequ in v er. 3.01: An integ ra ted so ftware package for popu lation genetics da ta analysis [ J]. Evo lutionary B io info rm atics On line, 2005, 1: 47-50.
[ 15]Rozas J, Sanchez -De lBarrio J C, M esseguer X, et a.l DnaSP, DNA po lym orphism analyses by the coa lescent and o ther m e thods[ J]. B io info rma tics, 2003, 19( 18): 2 496-2 497.
[ 16]汪江一, 徐芬, 刘绪生, 等. 基于部分18S rDNA, 28S rDNA 和CO I基因序列的索科线虫亲缘关系[ J]. 动物学报, 2007, 53( 5): 835-844.
[ 17]孔晓瑜, 喻子牛, 刘亚军, 等. 中华绒螯蟹与日本绒螯蟹线粒体COI基因片段的序列比较研究[ J]. 青岛海洋大学学报, 2001, 31( 6): 861-866.
[ 18]徐敬明, 张俊丽, 方华华, 等. 相手蟹属两种蟹类线粒体16S rDNA基因序列的比较[ J]. 水产学报, 2006, 25( 9): 443-447.

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备注/Memo

备注/Memo:
基金项目: 江苏省高校自然科学研究项目( 09KJD180005, 07K JA18017)、盐城师范学院校级项目( XKR2010003) . 通讯联系人: 唐伯平, 教授, 研究方向: 动物系统学. Email:boptang@ 163. com
更新日期/Last Update: 2013-04-08