[1]毕可然,顾伟,王文,等.基于16S rRNA和16S-23S rRNA基因间隔区序列探讨陆生和水生螺原体菌株的系统发生关系[J].南京师大学报(自然科学版),2011,34(01):96-101.
　Bi Keran,Gu Wei,Wang Wen,et al.Phylogenetic Relationship of Terrestrial and Aquatic Spiroplasmas Inferred From Their 16S rRNA and 16S-23S rRNA Intergenic Spacer Region Sequences[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(01):96-101.
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基于16S rRNA和16S-23S rRNA基因间隔区序列探讨陆生和水生螺原体菌株的系统发生关系()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年01期
页码:
96-101
栏目:
生命科学
出版日期:
2011-03-20

文章信息/Info

Title:
Phylogenetic Relationship of Terrestrial and Aquatic Spiroplasmas Inferred From Their 16S rRNA and 16S-23S rRNA Intergenic Spacer Region Sequences
作者:
毕可然1;  顾伟2;  王文2;  阎斌伦1;  张晓君1
1. 淮海工学院海洋学院, 江苏省海洋生物技术重点建设实验室, 江苏连云港222005 2. 南京师范大学生命科学学院, 江苏省生物资源技术和水生甲壳动物病害重点实验室, 江苏南京210046
Author(s):
Bi Keran1; Gu Wei2; Wang Wen2; Yan Binlun1; Zhang Xiaojun1
1.Jiangsu Key Laboratory of Marine Biotechnology,College of Oceaneering,Huaihai Institute of Technology,Lianyungang 222005,China 2. J iangsu K ey Laboratory for B ioresou rce and Technology and J iangsu Key Laboratory for Aquatic C rustacean D iseases, Sc
关键词:
螺原体;  16S rRNA基因;  16S??23S rRNA基因间隔区序列;  系统发生关系
Keywords:
spirop lasm a;  16S rRNA gene;  16S??23S rRNA in tergenic spacer reg ion sequences;  phy logene tic re la tionsh ip
分类号:
S945
摘要:
采集江苏省养殖池塘内发病的中华绒螯蟹、克氏原螯虾和南美白对虾, 利用已有螺原体16S rRNA和 23S rRNA基因序列保守引物扩增并测序16S rRNA和16S-23S rRNA基因间隔区序列( ISRs). 基于已获得的序列和GenB ank中下载的螺原体序列分别进行同源性比对, 比对的结果分别利用PAUP软件和M rBayes软件提供的最大似然法和贝叶斯法进行系统发生关系分析. 分析结果是: 所有淡水甲壳动物螺原体菌株Sp irop lasm a er iocheiris (中华绒螯蟹)、Sp irop lasma sp. CRAYFISH (克氏原螯虾)、Sp irop lasm a sp. SHRIMP(南美白对虾) 严格聚为一支, 并且与陆生兔扁虱螺原体(非凡螺原体) Sp irop lasma m irum 关系最近, 而与中国报道的陆生植物螺原体菌株Sp irop lasm a sp. CR-1 (油菜)、Sp irop lasma sp. CNR-1 ( 油菜)、Sp irop lasma sp. CNR-2 (油菜)、Sp irop lasm a sp. CAN-1(杜鹃花)、Sp irop lasma sp. CRW-1(红花酢浆草) 及昆虫螺原体菌株Sp irop lasma sp. CH-1(蜜蜂) 、Sp irop lasma sp. M10( 蜜蜂)关系较远, 此外, 3个淡水甲壳动物螺原体菌株与现今唯一报道的海水南美白对虾螺原体Sp i rop lasm a penaei系统发生关系也很远. 因此, 在螺原体属中, 我国发现的淡水甲壳动物螺原体近缘种是非凡螺原体而不是我国陆生昆虫或植物螺原体及美洲的南美白对虾螺原体.
Abstract:
The d iseased E riocheir sinens is, Procambarus clarki and P enaeus vannam ei w ere co llec ted from fa rm ponds in Jiangsu prov ince. The 16S rRNA and in tergenic spacer reg ion sequences were amp lified and sequenced using the conserved pr ime rs of 16S and 23S rRNA gene sequence for spirop lasm a. The obta ined sequences were a ligned com bined w ith the hom o logous sequences of a lm ost all species in the genus Sp irop lasma. The a lignm ent resu lts were analyzed by m ax imum like lihood and Bayesian m ethods. The resultsw ere as follow s: a ll spirop lasm a stra ins from freshw ater crustace ans, Sp irop lasm a eriocheiris (E riocheir sinens is), Sp irop lasm a sp. CRAYFISH (P ro cam barus clark i), Sp irop lasma sp. SHR IM P (Penaeu s vannam ei), we re str ic tly c luste red as a phylogenetic clade, and showed am ore c lose ly phy log enetic re lationsh ip w ith Sp irop lasma m irum than Sp irop lasma sp. CR-1 (Brassica camp estr is), Sp irop lasma sp. CNR-1 (Brassi ca camp estris ), Sp irop lasma sp. CNR-2 (B rassica camp estris ), Sp irop lasm a sp. CAN-1 (Rhododendron sim sii), Sp irop lasm a sp. CRW-1 (Oxalis corym bosa ) and Sp irop lasm a sp. CH-1 (Ap ism ellifera), Sp irop lasma sp. M 10 (Ap ism ellifera ), as we ll as Sp irop lasma p enaei. The c losely re la ted spec ies o f the sp iroplasm a strains from freshw ater crustaceans was not the spirop lasm a from terrestria l p lant, insect or ma rineP enaeus vannam ei but Sp irop lasma m irum.

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备注/Memo

备注/Memo:
基金项目: 生物多样性与生物技术江苏省重点实验室开放基金( 164070302104)、淮海工学院校内课题(KX08046) . 通讯联系人: 王文, 教授, 研究方向: 水生生物病原微生物学. E-mail:njnuwang@ 263. net
更新日期/Last Update: 2013-04-11