[1]王世娟,朱超云,宋伟,等.基于酪氨酸酶-氧化石墨烯的生物传感器的制备及应用[J].南京师大学报(自然科学版),2011,34(03):79-83.
 Wang Shijuan,Zhu Chaoyun,Song Wei,et al.Fabrication of Biosensor Based on Tyrosinase-Graphene Oxide and Its Application[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(03):79-83.
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基于酪氨酸酶-氧化石墨烯的生物传感器的制备及应用()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年03期
页码:
79-83
栏目:
化学
出版日期:
2011-09-20

文章信息/Info

Title:
Fabrication of Biosensor Based on Tyrosinase-Graphene Oxide and Its Application
作者:
王世娟朱超云宋伟李玉龙曹国庆倪超吴晓琼
王世娟,朱超云,宋伟,李玉龙,曹国庆,倪超,吴晓琼
Author(s):
Wang ShijuanZhu ChaoyunSong WeiLi YulongCao GuoqingNi ChaoWu Xiaoqiong
Department of Applied Chemistry,Nanjing College of Chemical and Technology,Nanjing 210048,China
关键词:
酪氨酸酶氧化石墨烯邻苯二酚生物传感器
Keywords:
tyrosinasegraphenecatecholbiosensor
分类号:
TP212.3
摘要:
研制了基于酪氨酸酶和氧化石墨烯修饰丝网印刷电极的生物传感器,并用于水中邻苯二酚的测定.1-芘丁酸琥珀酰亚胺酯分子通过非共价的π-π堆积作用吸附到氧化石墨烯上,酪氨酸酶与1-芘丁酸琥珀酰亚胺酯形成酰胺键,组装到氧化石墨烯上,制得纳米复合材料.该材料为酪氨酸酶的固定提供了有利的微环境,并且能很好地保持其生物活性.传感器对邻苯二酚响应灵敏、快速,达到稳态电流时间在9 s以内.实验结果表明,传感器的重现性、选择性和稳定性良好,检测线性范围为8.1×10-8 mol/L~2.2×10-5 mol/L,检出限为4.7
Abstract:
A disposable biosensor has been developed for the determination of catechol in water based on tyrosinase-graphene oxide modified screen printed electrode. Graphene oxide sheets ( GS) were noncovalently-functionalized with 1- pyrenebutanoic acid and succinimidyl ester ( PANHS) . Subsequently,the tyrosinase could covalently bind with PANHSfunctionalized GS by forming amide bond. The as-prepared biocompatible nanocomposite could provide a biocompatible microenvironment to protect the activity of tyrosinase effectively. This disposable biosensor exhibited a fast amperometric response ( less than 9 s) ,and a high sensitivity for monitoring catechol with good repeatability,selectivity and stability. The method showed good linearity in the range from 8. 1 × 10 - 8 mol /L to 2. 2 × 10 - 5 mol /L catechol with a detection limit of 4. 7 × 10 - 8 mol /L. The results showed that the as-prepared disposable biosensor could be used in the rapid and in-situ monitoring of phenolic compounds in water samples.

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备注/Memo

备注/Memo:
基金项目:南京化工职业技术学院科研基金重点项目( NHKY - 2010 - 05) .通讯联系人:朱超云,讲师,研究方向: 生物传感器在环境监测中的应用. E-mail: zhuchaoyun120@ gmail. Com
更新日期/Last Update: 2011-09-15