[1]吴寅子,张丽,温传俊.人NRAGE基因启动子转录活性的初步研究[J].南京师大学报(自然科学版),2011,34(03):90-94.
 Wu Yinzi,Zhang Li,Wen Chuanjun.Transcription Activity of Human NRAGE Gene Promoter[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(03):90-94.
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人NRAGE基因启动子转录活性的初步研究()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年03期
页码:
90-94
栏目:
生命科学
出版日期:
2011-09-20

文章信息/Info

Title:
Transcription Activity of Human NRAGE Gene Promoter
作者:
吴寅子张丽温传俊
南京师范大学生命科学学院分子细胞生物学研究所,江苏南京210046
Author(s):
Wu YinziZhang LiWen Chuanjun
Institute of Molecular Cell Biology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
NRAGE双荧光素酶法缺失突变转录活性
Keywords:
NRAGEdual-luciferase reporter assaydeletion mutantiontranscription activity
分类号:
Q78
摘要:
利用生物信息学软件搜寻NRAGE启动子区域的转录因子结合位点,并根据这些位点利用PCR技术克隆NRAGE启动子不同区域的缺失突变体,分别插入Luciferase报告载体pGL3-Basic载体中,构成8种缺失突变体的报告基因表达载体.通过双荧光素酶体系检测在HEK-293细胞中NRAGE启动子不同区域的转录活性,从而确定起主要作用的启动子区域.结果发现NRAGE基因转录起始位点上游-300 bp到-100 bp的区域起到主要的转录调控作用.
Abstract:
We analyzed human NRAGE promoter region by bioinformatic software and found putative binding sites of transcription factors,then 8 different fragments were amplified by PCR and the mutant promoters were inserted to upstream of the luciferase gene in luciferase report vector pGL3-Basic and detected the transcription activity by dual-luciferase reporter assay systerm. At transcription level we detected that transcription enhancer elements may exist in the region - 300 bp to - 100 bp.

参考文献/References:

[1] Imai Y,Shichijo S,Yamada A,et al. Sequence analysis of the MAGE gene family encoding human tumor-rejection antigens [J]. Gene,1995,60( 2) : 287-290.
[2] Zhang C G,Xin G C,Wei H D, et al. A new melanoma antigen-encoding gene subfamily in human chromosome X[J]. Acta Genetica Sinica,2001,28( 3) : 197-203.
[3] Kendall S E,Goldhawk D E,Kubu C,et al. Expression analysis of a novel p75NTR signaling protein,which regulates cell cycle progression and apoptosis[J]. Mech Dev,2002,117( 1 /2) : 187-200.
[4] Salehi A H,Roux P P,Kubu C J,et al. NRAGE,a novel MAGE protein,interacts with the p75 neurotrophin receptor and facilitates nerve growth factor dependent apoptosis[J]. Neuron,2000,27( 2) : 279-288.
[5] Wen C J,Xue B,Qin W X,et al. hNRAGE,A human neurotrophin receptor interacting MAGE homologue,regulates p53 transcriptional activity and inhibits cell proliferation[J]. FEBS Lett,2004,564( 1 /2) : 171-176.
[6] Salehi A H,Xanthoudakis S,Barker P A. NRAGE,a p75 neurotrophin receptor interacting protein,induces caspase activation and cell death through a JNK dependent mitochondrial pathway[J]. J Biol Chem,2002,277( 50) : 48 043-48 050.
[7] Williams M E,Strickland P,Watanabe K,et al. UNC5H1 induces apoptosis via its Juxtamembrane region through an interaction with NRAGE[J]. Biol Chem,2002,278( 19) : 17 483-17 490.
[8] Masuda Y,Sasaki A,Shibuya H,et al. Dlxin-1,a novel protein that binds Dlx5 and regulates its transcriptional function [J]. Biol Chem,2001,276( 7) : 5 331-5 338.
[9] Bertrand M J M,Kenchappa R S,Andrieu D,et al. NRAGE,a p75NTR adaptor protein,is required for developmental apoptosis in vivo[J]. Cell Death Differ,2008,15( 12) : 1 921-1 929.
[10] Matluk N,Rochira J A,Karaczyn A,et al. A role for NRAGE in NF-kappaB activation through the non-canonical BMP pathway[J]. BMC Biology,2010,8: 7-16.
[11] Chu C S,Xue B,Tu C,et al. NRAGE suppresses metastasis of melanoma and pancreatic cancer in vitro and in vivo[J]. Cancer Lett,2007,250( 2) : 268-275.
[12] Du Q,Zang Y,Tian X X,et al. MAGE-D1 inhibits proliferation,migration and invasion of human breast cancer cells[J]. Oncol Rep,2009,22( 3) : 659-665.
[13] Xue B,Wen C J,Shi Y,et al. Human NRAGE disrupts E-cadherin /b-catenin regulated homotypic cell-cell adhesion,Biochem. Biophys[J]. Res Commun,2005,336( 1) : 247-251
[14] 张成岗,贺福初. 黑色素瘤抗原编码基因家族新成员MAGED1 的组织表达谱研究[J]. 中国生物化学与分子生物学报,2002,18( 2) : 165-171.

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备注/Memo

备注/Memo:
基金项目:国家自然科学基金( 30771066) .通讯联系人:吴寅子,硕士研究生,研究方向: NRAGE 在生长发育及疾病发生中的作用. E-mail: wuyinzi - 1986@126. Com
更新日期/Last Update: 2011-09-15