[1]唐萍艳,刘吉华,余伯阳.复合诱变选育9α-羟基青蒿素高转化率菌株[J].南京师大学报(自然科学版),2011,34(03):107-110.
 Tang Pingyan,Liu Jihua,Yu Boyang.Breeding of the High 9α-Artemisinin Transformation Ratio Strain by Compound Mutation[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(03):107-110.
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复合诱变选育9α-羟基青蒿素高转化率菌株()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年03期
页码:
107-110
栏目:
生命科学
出版日期:
2011-09-20

文章信息/Info

Title:
Breeding of the High 9α-Artemisinin Transformation Ratio Strain by Compound Mutation
作者:
唐萍艳刘吉华余伯阳
中国药科大学中药复方实验室,江苏南京211198
Author(s):
Tang PingyanLiu JihuaYu Boyang
Department of Complex Prescription of TCM,China Pharmaceutical University,Nanjing 211198,China
关键词:
9α-羟基青蒿素复合诱变选育
Keywords:
9α-hydroxy-artemisinincompound mutationbreeding
分类号:
TQ927
摘要:
采用紫外和微波诱变方法对具有转化青蒿素产生9α-羟基青蒿素活性的链霉菌ATCC13273进行处理,以含有青蒿素为唯一碳源的筛选培养基进行选育.结果表明:通过紫外微波复合诱变的方法可以有效地提高ATCC13273的生物转化率,9α-羟基青蒿素的产率为50.7%,约为原菌株的2倍.
Abstract:
To increase the 9α-hydroxy-artemisinin transformation ratio,fermentated Streptomyces griseus ATCC-13273 in the way of ultraviolet( UV) and microwave compound mutation,and bred it by medium which uses artemisinin as the only carbon source. It proved that it is an effective way to increase the ratio of Streptomyces griseus ATCC-13273 transforms artemisinin into 9α-hydroxy-artemisinin by UV ray and microwave compound mutation,the yield of 9α-hydroxy-artemisinin is 50. 7%,which is almost twice higher than the initial strain’s.

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备注/Memo

备注/Memo:
基金项目:江苏省“六大人才高峰”资助项目.通讯联系人:唐萍艳,硕士研究生,研究方向: 中药活性生物转化. E-mail: boyangyu59@163. Com
更新日期/Last Update: 2011-09-15