[1]黄银华,黄修沦,温传俊.NRAGE基因甲基化调控其在乳腺癌细胞中的表达[J].南京师大学报(自然科学版),2011,34(04):89-95.
 Huang Yinhua,Huang Xiulun,Wen Chuanjun.Methylation of NRAGE Regulates Its Expression in the Breast Cancer Cell Lines[J].Journal of Nanjing Normal University(Natural Science Edition),2011,34(04):89-95.
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NRAGE基因甲基化调控其在乳腺癌细胞中的表达()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第34卷
期数:
2011年04期
页码:
89-95
栏目:
生命科学
出版日期:
2011-12-20

文章信息/Info

Title:
Methylation of NRAGE Regulates Its Expression in the Breast Cancer Cell Lines
作者:
黄银华黄修沦温传俊
南京师范大学生命科学学院分子细胞生物学研究所,江苏南京210046
Author(s):
Huang YinhuaHuang XiulunWen Chuanjun
Institute of Molecular Cell Biology,School of Life Sciences,Nanjing Normal University,Nanjing 210046,China
关键词:
NRAGE表达调控DNA 甲基化
Keywords:
NRAGE genegene regulationDNA methylation
分类号:
R737.9
摘要:
目的:探讨人NRAGE(Neurotrophin receptor-interacting MAGE homologue)基因启动子区CpG岛甲基化状态及其在乳腺癌细胞中的表达调控.方法:用Real-time PCR检测NRAGE在乳腺癌细胞MDA-MB-231和MCF-7中的表达;软件分析NRAGE启动子区是否存在CpG岛;脱甲基化药物5-Aza-CdR处理MDA-MB-231细胞和MCF-7细胞,半定量RT-PCR法及Western blot法检测用药前后细胞中NRAGE的mRNA及蛋白表达的变化;M
Abstract:
Objective: To detect the methylation status of the 5pCpG island located in the promoter region of hNRAGE gene and its regulation of the expressions of NRAGE in the breast cancer cells. Methods: The Real-time PCR method was used to detect the mRNA levels of NRAGE gene in the breast cancer cell lines of MDA-MB-231 and MCF-7. The existence of CpG island of hNRAGE gene was analysed by the Methyl Primer Express Software( Applied Biosystems) . Human breast cancer cell lines of MDA-MB-231 and MCF-7 were treated with 5-Aza-CdR. The expression of NRAGE was then detected by the semi-quantitative polymerase chain reaction ( RT-PCR) and Westen blot analysis. The methylation of NRAGE gene in the two kinds of cell lines was detected by the methylation-specific polymerase chain reaction ( MSP) . Results: CpG island existed in the promoter region of NRAGE gene. The CpG island of NRAGE was methylated in MDA-MB-231 cells and unmethylated in MCF-7 cells. The expression of mRNA level in MDA-MB-231 cells was higher than in MCF-7 cells. The RT-PCR and Westen blot analysis showed that NRAGE expression was increased significantly in MDA-MB-231 cells but remained the same in MCF-7 cells after exposuring to the demethylating agent 5-Aza- CdR. Conclusion: The methylation status of NRAGE gene can regulate its expression in the breast cancer cell lines.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金( 30771066) .通讯联系人: 黄银华,硕士研究生,研究方向: NRAGE 基因在生长发育及疾病发生中的作用. E-mail: huangyinhua1986@163. Com
更新日期/Last Update: 2013-03-21