[1]解文利,李鹏,靳芳草,等.中华绒螯蟹内源性白斑综合症病毒基因EjsWSSV的克隆与鉴定(英文)[J].南京师大学报(自然科学版),2012,35(04):63-72.
　Xie Wenli,Li Peng,Jin Fangcao,et al.Molecular Cloning and Characterization of an Endogenous White Spot Syndrome Virus Gene from Eriocheir Japonica Sinensis[J].Journal of Nanjing Normal University(Natural Science Edition),2012,35(04):63-72.
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中华绒螯蟹内源性白斑综合症病毒基因EjsWSSV的克隆与鉴定(英文)()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第35卷
期数:
2012年04期
页码:
63-72
栏目:
生命科学
出版日期:
2012-12-20

文章信息/Info

Title:
Molecular Cloning and Characterization of an Endogenous White Spot Syndrome Virus Gene from Eriocheir Japonica Sinensis
作者:
解文利;李鹏;靳芳草;周开亚;
南京师范大学生命科学学院，江苏省生物多样性和生物技术重点实验室，江苏南京210023
Author(s):
Xie Wenli; Li Peng; Jin Fangcao; Zhou Kaiya
Jiangsu Key Laboratory for Biodiversity and Biotechnology,School of Life Sciences,Nanjing Normal University,Nanjing 210023,China
关键词:
中华绒螯蟹; 白斑综合症病毒;  cDNA 末端快速扩增;  cDNA 克隆; 序列分析
Keywords:
Eriocheir japonica sinensis; WSSV; RACE;  cDNA cloning;  sequence analyses
分类号:
S945
摘要:
基于已获得的表达序列标签序列,应用RACE技术从中华绒螯蟹基因组中获得了一个白斑综合症病毒基因(EjsWSSV)的全长cDNA序列,全长为3 864 bp并包含一个3 732 bp的开放阅读框,编码一个138.25 kDa的含1 243个氨基酸的多肽,编码的氨基酸序列包含一个VWA结构域.通过序列比对、序列结构比较和一些生物信息学预测分析,表明EjsWSSV蛋白序列与白斑综合症病毒基因组的ORF16具有高同源性,它不含有信号肽和跨膜区,为非分泌型和α-螺旋型蛋白,可能定位于细胞质.通过PCR检测验证,Ej
Abstract:
Based on expressed sequence tag( EST) and rapid amplification of cDNA ends( RACE) techniques，a WSSV gene designated as EjsWSSV was cloned and characterized from the Chinese mitten crab Eriocheir japonica sinensis． The full-length cDNA of EjsWSSV is 3 864 bp in size and contains an open reading frame of 3 732 bp which encodes a 1 243 amino acid polypeptide( 138. 25 kDa) with VWA domain． Sequence alignment， structure comparison and some bioinformatics analyses showed that EjsWSSV shared 100% similarity with WSSV genome ORF16 sequence． The bioinformatics analyses revealed that EjsWSSV had no putative signal peptide found and indicated that it was probably a non-secretory， alpha spiral and non-transmembrane protein． It was probably located in the cytoplasm． The PCR amplification results indicated that EjsWSSV was probably an endogenous virus gene of E． j． sinensis．

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备注/Memo

备注/Memo:
基金项目: Jiangsu Province Basic Research Program( Natural Science Foundation Funded Project( BK2010541) ) ．通讯联系人: Li Peng，lecturer，majored in molecular evolution and comparative genomics． E-mail: lipeng@ njnu． edu． cn
更新日期/Last Update: 2013-03-11