[1]田亲亲,阎丽娜,陆长梅,等.籼稻不同品种成熟胚的组培发育特性及其超微结构的观察[J].南京师大学报(自然科学版),2013,36(01):68-73.
　Tian Qinqin,Yan Lina,Lu Changmei,et al.Comparison of Ultrastructure of Callus Induction in Indica Rice With Different Callus Induction Rates[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(01):68-73.
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籼稻不同品种成熟胚的组培发育特性及其超微结构的观察()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年01期
页码:
68-73
栏目:
生命科学
出版日期:
2013-03-31

文章信息/Info

Title:
Comparison of Ultrastructure of Callus Induction in Indica Rice With Different Callus Induction Rates
作者:
田亲亲1; 2; 阎丽娜2; 陆长梅1; 李 霞2; 魏晓东2; 方先文2
(1.南京师范大学生命科学学院,江苏 南京 210023) (2.江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,江苏 南京 210014)
Author(s):
Tian Qinqin1; 2; Yan Lina2; Lu Changmei1; Li Xia2; Wei Xiaodong2; Fang Xianwen2
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China) (2.Institute of Foods Crop,Jiangsu Academy of Agricultural Sciences,Jiangsu High Quality Rice R&D Center,Nanjing 210014,China)
关键词:
籼稻; 成熟胚; 愈伤组织; 超微结构
Keywords:
Indica rice; mature embryo; callus tissue; ultrastructure
分类号:
S511
摘要:
以3种籼稻成熟胚为外植体,接种于愈伤组织诱导培养基(M8+30 g/L蔗糖+8 g/L琼脂+2 mg/L 2,4-D+2 mg/L 6-BA+1 mg/L ABA+0.5 mg/L KT+1 mg/L NAA),记录其愈伤的出愈率,统计分析表明其出愈率有显著差异,并分别取接种9 d、15 d时不同材料的愈伤组织观察其不同诱导天数下超微结构的变化,并记录其愈伤过程中的褐化率.结果显示,3种水稻材料在相同的诱导时间下细胞的发育阶段不同:扬辐籼6号的愈伤组织细胞发育较快,较早地进入了成熟阶段; 而恢76愈伤组织细胞则发育较慢,较长时间仍然处于分生阶段; 黄华占则发育更慢.可见,不同籼稻出愈率和分化的高低主要与愈伤组织细胞发育速度的快慢、生理功能的强弱、以及其衰老的程度有密切关系.而褐化,不仅与黑色物质聚集早晚有关系,而且与愈伤组织细胞中液泡的发育有着密切关系.通过调高培养基中细胞分裂素类的含量,有利于提高其出愈率.
Abstract:
Mature embryos from 3 Indica rice varieties as explants were implanted in the induction medium(M8+30 g/L Sucrose+10 g/L Agar+5 g/L Sorbitol+2 mg/L 2,4-D+2 mg/L 6-BA+1 mg/L ABA+0.5 mg/L KT+1 mg/L NAA)and the callus inductivities of these materials were different significantly at 0.01 level,respectively.At the same time,the callus incubated for 9 d and 15 d were observed by a transmission electron microscope,and the callus’browning rate was recorded at the same time.The results showed that the 3 rice materials developed differently at the same induction period.The callus cells from Yangfuxian 6 developed fast,while those from Hui 76 developed relatively slow,and the development of callus cells from Huanghuazhan was the slowest.The performances in callus ultrastructure were also consistent with their cell development periods.It suggested that the callus induction in Indica rice had close relation with their cell development rates,physiological functions and senescences as well.The callus browning was also related to the accumulation period of some black substance and the development of their vacuoles.The rate of callus induction in Indica rice would be increased by adding cytokinin in medium for callus induction.

参考文献/References:

[1] Li F,Cui X,Feng Z,et al.The effect of 2,4-D and kinetin on differentiation of petiole cells in Arabidopsis thaliana[J].Biologia Plantarum,2012,56(1):121-125.
[2]Yan L,Li X,Wu D.The comparison in tissue culture ability of mature embryo in different cultivars of rice[J].Agricultural Sciences in China,2010,9(6):840-84.
[3]Lakshmanan P.Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane(Saccharum spp.interspecific hybrids)leaf culture[J].Plant Cell Rep,2006,25(10):1007-1015.
[4]魏晓东,李霞,姚殊,等.糯性和非糯性水稻品种成熟胚组织培养力的比较[J].核农学报,2012,26(1):6-10.
[5]李霞,陈婷,周月兰.籼粳稻成熟胚愈伤组织培养力的比较[J].南京师大学报:自然科学版,2005,28(4):103-108.
[6]阎丽娜,李霞,蔡庆生.水稻武育粳3号成熟胚愈伤组织诱导及植株再生研究[J].江苏农业科学,2009(6):67-69.
[7]危晓薇,王冬梅,祖木热木·吐尔逊.水稻成熟胚愈伤组织诱导及其植株再生[J].新疆农业科学,2007,44(6):889-891.
[8]任永刚,张建中,张红梅,等.通过成熟胚离体获得巨胚水稻新品种及性状和稻米品质分析[J].上海师范大学学报:自然科学版,2011,40(3):289-294.
[9]黄绿红,单杨,李高阳,等.铜元素对籼稻成熟胚组织培养的影响[J].湖南农业科学,2010(7):6-8,12.
[10]苗春波,万志刚,孙丙耀.2,4-D和6-BA对籼稻成熟胚愈伤组织诱导和植株再生的影响[J].安徽农业科学,2009,37(27):12927-12929.
[11]覃伟,韦鹏霄,岑秀芬,等.提高优质籼稻成熟胚离体培养效果的几个因子[J].广西农业科学,2005,36(3):193-195.
[12]殷绪明,徐庆国,李海林.籼型杂交稻亲本成熟胚愈伤组织再生体系的建立[J].现代生物医学进展,2007,7(3):347-350.
[13]王萍,徐大勇,王罡,等.粳籼稻两个亚种成熟胚组织培养与再生能力的比较研究[J].种子,2007,26(10):66-67.
[14]阎丽娜,李霞,吴丹.不同类型水稻材料成熟胚组织培养力的比较[J].中国农业科学,2010,43(6):1127-1135.
[15]宋春凤,孙大业,张玉英,等.白芷愈伤组织的电镜制样技术及超微结构观察[J].河北师范大学学报:自然科学版,1998,22(3):396-398.
[16]王超,李霞.高光强下转玉米PEPC基因水稻和未转基因水稻秧苗叶片超微结构的比较[J].华北农学报,2007,22(4):99-103.
[17]陈德海,潘文兰,杨涛.草莓组织培养细胞发育过程的电镜观察[J].厦门大学学报:自然科学版,1999,38(6):918-923.
[18]陈颖,曹福亮,甘习华.不同状态银杏愈伤组织蛋白质电泳分析及电镜观察[J].西北植物学报,2006,26(11):2239-2243.
[19]杨涛,陈德海,吴荔萍.安祖花组织培养及其细胞和叶绿体发育过程的电镜观察[J].亚热带植物通讯,1998,27(1):1-7.
[20]阎丽娜,李霞.不同粳稻品种成熟胚诱导过程愈伤组织超微结构的比较[J].南京师大学报:自然科学版,2009,32(4):103-109.

相似文献/References:

[1]李霞,赵贇涛,阎丽娜,等.籼稻扬稻6号和粳稻苏沪香粳花药培养力的比较[J].南京师大学报(自然科学版),2008,31(04):108.
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备注/Memo

备注/Memo:
收稿日期:2012-05-16.
基金项目:江苏省自主创新课题[CX(11)1020]、国家自然科学基金项目(30871459).
通讯联系人:李霞,博士,研究员,研究方向:水稻生物技术研究.E-mail:jspplx@jaas.ac.cn
更新日期/Last Update: 2013-03-31