[1]陶明煊,周 斌,郭永月,等.姬菇多糖对急性酒精性肝损伤小鼠的肝组织超微结构的影响[J].南京师大学报(自然科学版),2013,36(01):85-89.
　Tao Mingxuan,Zhou Bin,Guo Yongyue,et al.Effects of Polysaccharides from Pleurotus Cornucopiae on Ultrastructure Change of Acute Alcoholic Injuried Liver in Mice[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(01):85-89.
点击复制

姬菇多糖对急性酒精性肝损伤小鼠的肝组织超微结构的影响()
分享到：

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年01期
页码:
85-89
栏目:
生命科学
出版日期:
2013-03-31

文章信息/Info

Title:
Effects of Polysaccharides from Pleurotus Cornucopiae on Ultrastructure Change of Acute Alcoholic Injuried Liver in Mice
作者:
陶明煊; 周 斌; 郭永月; 赵云霞
南京师范大学金陵女子学院,江苏 南京 210097
Author(s):
Tao Mingxuan; Zhou Bin; Guo Yongyue; Zhao Yunxia
Jinling College,Nanjing Normal University,Nanjing 210097,China
关键词:
姬菇多糖; 急性肝损伤; 肝组织; 超微结构
Keywords:
Pleurotus cornucopiae polysaccharides; acute liver injury; liver tissue; ultrastructure
分类号:
TS201.3
摘要:
目的:研究以热水浸提法从姬菇中提取的姬菇多糖(PCNP)对酒精所致的急性损伤小鼠肝组织超微结构的影响.方法:84只小鼠被随机分为空白对照组、模型组、阳性对照组(150 mg/kg·bw)、多糖各剂量组((100、200、400)mg/kg·bw).连续灌胃30 d后,给予浓度为50%的乙醇溶液,建立动物急性肝损伤模型.12 h后,小鼠脱臼处死,取肝脏制成超薄切片,电镜观察肝组织超微结构的变化.结果:模型组细胞核的核膜不规则、呈锯齿状,线粒体畸形、嵴不清楚、糖原增多、出现空泡,内质网扩张、其膜上附着的核糖体脱失,细胞核周围脂滴数明显增多、胆管微绒毛脱失; 姬菇多糖组肝细胞核膜凹凸不平,线粒体肿胀、线粒体内糖原沉积较少,内质网扩张不明显,细胞内脂滴减少.结论:姬菇多糖对酒精性肝损伤小鼠的肝细胞具有保护作用.
Abstract:
Objective:The present study investigated the protective effect of polysaccharide from Pleurotus cornucopiae(PCNP)on electron microscopic changes in acute alcoholic hepatic injury in mice.Methods:The 84 mice were randomly divided into blank control group,alcoholic model groups.The mice in alcoholic model groups were administered by gavage with BIFENDATE(150 mg/kg·bw)or PCNP((100、200、400)mg/kg·bw)for 30 d before the administration with dose of 50% alcohol(12 mL/kg·bw).After the last alcohol treament for 12 h,in order to observe the ultrastructural changes of liver cells,all mice were dislocation death to collect samples for preparing ultrathin section for electron microscopy.Result:In model group,hepatic cells exhibited in different degrees of karyopycnosis and the nuclear membranes were irregular; The pathological changes in mitochondria had been clearly observed.In the hepatic cell,vacuoles can be seen and glycogen was increased.The rough endoplasmic reticulum were found as expansion and degranulation; Many large lipid droplets were found around the nucleus and microvilli of bile duct lost.In the PCNP groups,karyolemma were uneven.There were less expansion of mitochondria and less glycogen deposition compared with other groups; In addition,endoplasmic reticulum was not obvious outspread and lipid droplets reduced in cells.Conclusion:It can be seen that PCNP has the protective effect on hepatic ultrastructure induced by alcohol.

参考文献/References:

[1] 鞠辉,魏良洲.酒精性肝病的流行病学现状[J].肝脏,2006,11(1):57-58.
[2]Reuben A.Alcohol and the liver[J].Current Opinion in Gastroenterology,2008,24(3):328-338.
[3]Day C P.Treatment of alcoholic liver disease[J].Liver Transplantation,2007,13(11Suppl.2):69-75.
[4]王波.彩色图解姬菇、肺形侧耳栽培新技术[M].成都:四川科学出版社,2003:1-2.
[5]郭勇,周洁,谭伟,等.我国姬菇研究现状[J].中国食用菌,2009,28(6):12-13.
[6]姜自彬,曹锦花,魏学彦.糙皮侧耳真菌的研究进展[J].中草药,1999,30(1):72-75.
[7]Eluhan S,Ranogajec A.Composition and non-volatile components of croatian wild edible mushrooms[J].Food Chemistry,2011,124(3):1076-1082.
[8]Elmasta M,Isildak O,Turkekul I,et al.Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms[J].Journal of Food Composition and Analysis,2007,20(3):337-345.
[9]Wasser S P.Medicinal mushrooms as a source of antitumor and immunomodulating polysaccharides[J].Microbiol Biotechnol,2002,60(3):258-274.
[10]王峰,陶明煊,程光宇,等.4种食用菌提取物自由基清除作用及降血糖作用的研究[J].食品科学,2009,30(21):343-347.
[11]Elbohi K,Sabik L,Muzandu K,et al.Antigenotoxic effect of Pleurotus cornucopiae extracts on the mutagenesis of Salmonella typhimurium TA98 elicited by benzo pyrene and oxidative DNA lesions in V79 hamster lung cells[J].Japanese Journal of Veterinary Research,2005,52(4):163-172.
[12]Khloodm E B,Yoshiharu H,Kaampwe M,et al.Protective effect of Pleurotus cornucopiae mushroom extract on carbon tetrachloride-induced hepatotoxicity[J].Japanese Journal of Veterinary Research,2009,57(2):109-118.
[13]徐莉.医学生物学[M].上海:上海科学技术出版社,2005.
[14]Betaller R,North K E,Brenner D A.Genetic polymorphisms and the progression of liver fibrosis:a critical appraisal[J].Hepatology,2003,37:493-503.
[15]Bergeron R,Previs S F,Cline G W,et al.Effect of 5-aminoimidazone-4-carbo-xamide-1-beta-D-ribofuranoside infusion on in vivo glucose and lipid metabolism in lean and obese Zucker rats[J].Diabetes,2001,50:1076-1082.
[16]Malhi H,Kaufman R J.Endoplasmic reticulum stress in liver disease[J].J Hepatol,2011,54(4):795-809.
[17]Beier J I,Mclain C J.Mechanisms and cell signaling in alcoholic liver disease[J].Biol Chem,2010,391(11):1249-1264.
[18]Song Z,Joshi B S,Barve S,et al.Advances in alcoholic liver disease[J].Curr Gastroenterol Rep,2004,6(1):71-76.
[19]刘洁,李文香,王文亮,等.多糖空间结构与生物活性相关性研究进展[J].农业机械,2011,6(17):153-155.
[20]黄芳,蒙义文.活性多糖的研究进展[J].天然产物的研究与开发,1999,11(5):90-97.
[21]朱良,沈耀威,赵冠欣,等.橄榄总黄酮对小鼠急性酒精性肝损伤的保护作用[J].食品与机械,2010,26(3):91-93.

相似文献/References:

[1]王美菊,陆文娟,喻 晨,等.不同提取方法对姬菇多糖抗氧化活性的影响[J].南京师大学报(自然科学版),2016,39(04):0.[doi:10.3969/j.issn.1001-4616.2016.04.012]
　Wang Meiju,Lu Wenjuan,Yu Chen,et al.The Effect of Antioxidant Activity on the Polysaccharides fromPleurotus cornucopiae with Different Extraction Ways[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(01):0.[doi:10.3969/j.issn.1001-4616.2016.04.012]
[2]唐娅秋,朱亚男,王美菊,等.姬菇多糖对免疫低下型小鼠肝脏和肾脏抗氧化作用的影响[J].南京师大学报(自然科学版),2019,42(04):85.[doi:10.3969/j.issn.1001-4616.2019.04.012]
　Tang Yaqiu,Zhu Yanan,Wang Meiju,et al.Effects of Acid Polysaccharides from Pleurotus cornucopiaeon the Antioxidation in Mice with Immunosuppression[J].Journal of Nanjing Normal University(Natural Science Edition),2019,42(01):85.[doi:10.3969/j.issn.1001-4616.2019.04.012]

备注/Memo

备注/Memo:
收稿日期:2012-06-08.
基金项目:江苏省高校自然科学基础研究项目(10KJD 550003)、江苏省普通高校研究生科研创新计划项目(CXLX11_0895).
通讯联系人:陶明煊,硕士,副教授,研究方向:生物活性物质与保健功能因子.E-mail:45017@njnu.edu.cn
更新日期/Last Update: 2013-03-31