[1]刘海龙,黄鹤勇.邻苯二甲酸为基质用于基质辅助激光解吸/电离飞行时间质谱测定小分子物质[J].南京师大学报(自然科学版),2013,36(03):57-60.
　Liu Hailong,Huang Heyong.Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using Phthalic Acid Matrix for Small Molecule Analysis[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(03):57-60.
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邻苯二甲酸为基质用于基质辅助激光解吸/电离飞行时间质谱测定小分子物质()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年03期
页码:
57-60
栏目:
化学
出版日期:
2013-09-30

文章信息/Info

Title:
Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Using Phthalic Acid Matrix for Small Molecule Analysis
作者:
刘海龙1; 黄鹤勇2
(1.南京师范大学生命科学学院,江苏 南京 210023) (2.南京师范大学分析测试中心,江苏 南京 210023)
Author(s):
Liu Hailong1; Huang Heyong2
(1.School of Life Science,Nanjing Normal University,Nanjing 210023,China) (2.Analysis and Testing Center,Nanjing Normal University,Nanjing 210023,China)
关键词:
基质辅助激光解吸/电离飞行时间质谱; 多巴胺; 基质; 邻苯二甲酸
Keywords:
matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-TOF MS); dopamine; matrix; Phthalic acid
分类号:
O657.63
摘要:
以邻苯二甲酸为基质对多巴胺(153.08 Da)进行基质辅助激光解吸/电离飞行时间质谱(MALDI TOF-TOF MS)分析.结果表明,邻苯二甲酸作为基质可以有效解吸电离多巴胺,基质干扰峰明显减少,产生出了分辨率高、离子信号强、信噪比(S/N)高的质谱图,不仅准确测定了多巴胺的相对分子质量,完成了对其定性研究,还找到了一种适合小分子分析而且来源广泛的基质材料.
Abstract:
Phthalic acid was studied as matrices for determination of dopamine(153.08 Da)samples by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI TOF-TOF MS)method.This matrix material displayed at least two advantages.First,it was possible to avoid low mass chemical noise arising from the matrix in positive-ion mode.Second,phthalic acid could be used conventionally due to the low cost.The MALDI-TOF-MS analysis of dopamine demonstrated that the matrix material based on phthalic acid yielded spectra with minimal chemical noise and good analysis signal intensity.The present method can be readily applied to obtain the low background noise MALDI-TOF mass spectra of small molecules compounds.

参考文献/References:

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相似文献/References:

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备注/Memo

备注/Memo:
收稿日期:2013-02-25.
基金项目:江苏省科技基础研究基金(bm2007132).
通讯联系人:刘海龙,助理实验师,研究方向:生物质谱分析.E-mail:lhl7083609@163.com
更新日期/Last Update: 2013-09-30