[1]张燕萍,邵 芳,郑红梅,等.松江鲈MRF4基因克隆与组织表达分析[J].南京师大学报(自然科学版),2013,36(03):108-115.
 Zhang Yanping,Shao Fang,Zheng Hongmei,et al.Cloning and Tissue Expression Analyses of MRF4 Gene in Trachidermus Fasciatus[J].Journal of Nanjing Normal University(Natural Science Edition),2013,36(03):108-115.
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松江鲈MRF4基因克隆与组织表达分析()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第36卷
期数:
2013年03期
页码:
108-115
栏目:
生命科学
出版日期:
2013-09-30

文章信息/Info

Title:
Cloning and Tissue Expression Analyses of MRF4 Gene in Trachidermus Fasciatus
作者:
张燕萍邵 芳郑红梅卢祥云徐建荣顾志良
常熟理工学院生物与食品工程学院,江苏 常熟 215500
Author(s):
Zhang YanpingShao FangZheng HongmeiLu XiangyunXu JianrongGu Zhiliang
College of Biological Science and Food Technology,Changshu Institute of Technology,Changshu 215500,China
关键词:
松江鲈MRF4RACE组织表达分析
Keywords:
Trachidermus fasciatusMRF4RACEtissue expressional pattern
分类号:
Q38; S917.4
摘要:
MRF4(myf6或herculin)基因属于MyoD家族成员之一,为成肌细胞分化成肌管的重要调控因子.本实验以松江鲈(Trachidermus fasciatus)肌肉总RNA为模板,采用RT-PCR结合RACE的方法,获得1 049 bp全长cDNA序列,包含了1个含有720个核苷酸的开放性阅读框(ORF),共编码239个氨基酸.其编码区序列与石斑鱼同源性最高、为91.81%,其次是东方红鳍鲀、为83.40%,与哺乳类、鸟类的同源性在65%左右,与爪蟾同源性最低仅为59.65%.预测松江鲈MRF4蛋白具有保守的HLH结构域,bHLH结构域形成剪刀状α-螺旋二聚体.用荧光定量PCR方法检测发现,MRF4基因的mRNA在松江鲈6种组织中均有表达,但其在肌肉组织中表达量极显著高于其他组织,提示其在肌肉的生长发育过程中起着重要作用.
Abstract:
The transcription factor MRF4 is one member of MyoD family which have essential functions in myogenic lineage determination and muscle differentiation.In order to obtain full-length MRF4 gene sequence and characterize its tissue expression profile in Trachidermus fasciatus,RT-PCR and rapid-amplification cDNA ends(RACE)were used.A full-length of 1049 bp MRF4 cDNA,which contained a 720 bp long complete ORF encoding a 239 amino acid peptide.Multiple sequence comparison showed that the T.fasciatus MRF4 had a high homology with Epinephelus coioides at 91.81%,followed by Takifugu rubripes.A homology with mammals and birds was at about 63%,and of the lowest homology with Xenopus laevis at 59.65%.The bHLH domain of T.fasciatus MRF4 was forming α-helix dimer that like a scissors,and HLH sequence was highly homologous among different species.MRF4 gene was detected in all 6 examined tissues,but its expression level in muscle was significantly higher than the amount of other tissues,implying that the fish MRF4 gene would play an important role in muscle development.

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备注/Memo

备注/Memo:
收稿日期:2012-09-23.
基金项目:教育部留学回国人员科研启动基金资助(第42批)、常熟市科技局项目(CN201111).
通讯联系人:顾志良,博士,教授,研究方向:动物基因组学与分子遗传学.E-mail:zhilianggu88@hotmail.com
更新日期/Last Update: 2013-09-30