[1]陈文娟,陈建福.超声辅助提取血柚皮多糖工艺优化及其对自由基的清除作用[J].南京师大学报(自然科学版),2014,37(01):141.
　Chen Wenjuan,Chen Jianfu.Optimization of Ultrasonic-Assisted Extracting and Free Radicals Scavenging of Polysaccharides from Blood Pummelo Peel[J].Journal of Nanjing Normal University(Natural Science Edition),2014,37(01):141.
点击复制

超声辅助提取血柚皮多糖工艺优化及其对自由基的清除作用()
分享到：

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第37卷
期数:
2014年01期
页码:
141
栏目:
生命科学
出版日期:
2014-03-30

文章信息/Info

Title:
Optimization of Ultrasonic-Assisted Extracting and Free Radicals Scavenging of Polysaccharides from Blood Pummelo Peel
作者:
陈文娟1; 陈建福2
(1.漳州城市职业学院食品工程系,福建 漳州 363000) (2.福州大学化学化工学院,福建 福州 350108)
Author(s):
Chen Wenjuan1; Chen Jianfu2
(1.Department of Food Engineering,Zhangzhou City University,Zhangzhou 363000,China) (2.College of Chemistry and Chemical Engineering,Fuzhou University,Fuzhou 350108,China)
关键词:
血柚皮; 多糖; 超声波辅助; 自由基清除
Keywords:
blood pummelo peel; polysaccharides; ultrasonic-assisted; free radicals scavenging
分类号:
R284.2; O636.1; TS201
文献标志码:
A
摘要:
以漳州血柚皮为原料,采用超声波辅助热浸提法对血柚皮多糖进行提取,并以苯酚—硫酸法测定多糖提取率.分别对超声功率、超声温度、料液比、超声时间进行单因素和正交试验,并通过极差、方差分析对提取过程显著影响提取率的因素进行统计分析.结果表明,超声波辅助热浸提取血柚皮中多糖的最佳工艺条件为:超声功率80 W、超声温度65 ℃、料液比1:30、超声时间70 min.该工艺条件下血柚皮多糖的提取率为18.51%,该方法的提取率比无超声波辅助的热浸提法提高了2.24%.以抗坏血酸为对照,考察了其羟自由基清除能力、DPPH自由基清除能力以及超氧阴离子自由基清除能力,结果显示,多糖对自由基的清除能力很强,但其对各自由基的清除能力均小于抗坏血酸.表明血柚皮多糖作为天然的抗氧化剂有一定的应用前景.
Abstract:
The polysaccharides from blood pummelo peel was abstracted by ultrasonic-assisted heat extraction,and the extraction yield was measured by coloration method with phenol-sulfuric acid.The effects of extraction yield on the parameters including ultrasonic power,ultrasonic temperature,material-to-liquid ratio,ultrasonic time were investigated by single factor test and orthogonal array design methods.And the notability difference was analyzed with the statistic method of range and variance.Results showed that the optimal conditions were as follows:ultrasonic power was 80 W,ultrasonic temperature was 65 ℃,material-to-liquid ratio was 1:30,ultrasonic time was 70 min.The extraction yield of the polysaccharides from blood pummelo peel was reached up to 18.51% and it increased 2.24% compared to the heat extraction without ultrasonic-assisted.Hydroxyl radical scavenging ability,DPPH scavenging ability and reducing capacity of polysaccharide were studied by taking ascorbic acid as a referential substance.The result showed that it had good free radicals scavenging activity,however,weaker antioxidant activity than ascorbic acid.These results clearly established the possibility that polysaccharide from blood pummelo peel should be effectively employed as the type of natural antioxidant.

参考文献/References:

[1] 陈文娟,陈建福.漳州血柚皮总黄酮的提取工艺研究[J].北京联合大学学报:自然科学版,2012,26(4):40-45.
[2]Sui Z Q,Gizaw Y,BeMiller J N.Extraction of polysaccharides from a species of Chlorella[J].Carbohyd Polym,2012,90(1):1-7.
[3]Na Y S,Kim W J,Kim S M,et al.Purification,characterization and immunostimulating activity of water-soluble polysaccharide isolated from Capsosiphon fulvescens[J].Int Immunopharmacol,2010,10(3):364-370.
[4]Sun Y X,Liu J C.Structural characterization of water-soluble polysaccharides from the roots of codonopsis pilosula and its immunity activity[J].Int J Boil Macromol,2008,43(3):279-282.
[5]陈文娟,曾稍俏,陈建福.漳州血柚皮水溶性多糖的水提工艺[J].河南科技学院学报:自然科学版,2013,41(2):31-35.
[6]Zhao Q,Kennedy J F,Wang X,et al.Optimization of ultrasonic circulating extraction of polysaccharides from Asparagus officinalis using response surface methodology[J].Int J Biol Macromol,2011,49(2):181-187.
[7]王建安,苑光辉,李艳芝,等.超声法提取金叶女贞果实中多糖工艺的研究[J].南京师大学报:自然科学版,2012,35(4):73-77.
[8]Tian Y T,Zeng H L,Xu Z B,et al.Ultrasonic-assisted extraction and antioxidant activity of polysaccharides recovered from white button mushroom(Agaricus bisporus)[J].Carbohyd Polym,2012,88(2):522-529.
[9]Peng Y F,Zhang L N,Zeng F B,et al.Structure and antitumor activity of extracellular polysaccharides from mecelium[J].Carbohyd Polym,2003,54(3):297-303.
[10]Zhong Z S,Wang F,Wang X M,et al.Extraction of the polysaccharides from five algae and their potential antioxidant activity in vitro[J].Carbohyd Polym,2010,82(1):118-121.
[11]Yang W J,Fang Y,Liang J,et al.Optimization of ultrasonic extraction of Flammulina velutipes polysaccharides and evaluation of its acetylcholinesterase inhibitory activity[J].Food Res Int,2011,44(5):1 269-1 275.
[12]Zhao Z Y,Xu X J,Ye Q W,et al.Ultrasound extraction optimization of Acanthopanax senticosus polysaccharides and its antioxidant activity[J].Int J Boil Macromol,2013,59(1):290-294.
[13]Zhang Z F,Lü G Y,He W Q,et al.Effects of extraction methods on the antioxidant activities of polysaccharides obtained from Flammulina velutipes[J].Carbohyd Polym,2013,98(2):1524-1531.
[14]Cheung Y C,Siu K C,Liu Y S,et al.Molecular properties and antioxidant activities of polysaccharide-protein complexes from selected mushrooms by ultrasound-assisted extraction[J].Process Biochem,2012,47(5):892-895.

相似文献/References:

[1]王建安,苑光辉,李艳芝,等.超声法提取金叶女贞果实中多糖工艺的研究[J].南京师大学报(自然科学版),2012,35(04):73.
　Wang Jianan,Yuan Guanghui,Li Yanzhi,et al.Optimum Extraction of Polysaccharides From Fruits of Ligustrum Vicaryi by Ultrasonic Method[J].Journal of Nanjing Normal University(Natural Science Edition),2012,35(01):73.
[2]魏 华,张 娣,陆 玲,等.几种食用菌多糖的提取与抗氧化性研究[J].南京师大学报(自然科学版),2017,40(02):72.[doi:10.3969/j.issn.1001-4616.2017.02.012]
　Wei Hua,Zhang Di,Lu Ling,et al.Extraction,Purification and Antioxidant Activities of Several Polysaccharides from Edible and Medicinal Fungi[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(01):72.[doi:10.3969/j.issn.1001-4616.2017.02.012]
[3]黄馨阅,丁慧敏,马士玄,等.红须腹菌多糖的提取、羧甲基化修饰及免疫活性研究[J].南京师大学报(自然科学版),2023,46(03):60.[doi:10.3969/j.issn.1001-4616.2023.03.009]
　Huang Xinyue,Ding Huimin,Ma Shixuan,et al.Extraction,Carboxymethylation Modification and in Vivo Immunomodulatory Effects of Polysaccharides from Rhizopogon Rubescens(Tul.)Tul[J].Journal of Nanjing Normal University(Natural Science Edition),2023,46(01):60.[doi:10.3969/j.issn.1001-4616.2023.03.009]

备注/Memo

备注/Memo:
收稿日期:2013-09-03.
基金项目:漳州市自然科学基金(ZZ2012J12).
通讯联系人:陈文娟,助教,硕士,研究方向:食品科学与工程.E-mail:chenwenjuan8491@126.com
更新日期/Last Update: 2014-03-30