[1]许 晔,刘 青,陈 曦,等.水通道蛋白在‘丰水’、‘今村秋’花粉萌发中的作用[J].南京师大学报(自然科学版),2014,37(04):99.
　Xu Ye,Liu Qing,Chen Xi,et al.The Role of Aquaporins in Housui and Imanuraaki Pollens Germination[J].Journal of Nanjing Normal University(Natural Science Edition),2014,37(04):99.
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水通道蛋白在‘丰水’、‘今村秋’花粉萌发中的作用()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第37卷
期数:
2014年04期
页码:
99
栏目:
生命科学
出版日期:
2014-12-31

文章信息/Info

Title:
The Role of Aquaporins in Housui and Imanuraaki Pollens Germination
作者:
许 晔; 刘 青; 陈 曦; 徐国华
江苏第二师范学院生命科学与化学化工学院,江苏省生物功能分子重点建设实验室,江苏 南京 210013
Author(s):
Xu Ye; Liu Qing; Chen Xi; Xu Guohua
Faculty of Life Sciences and Chemistry,Jiangsu Second Normal University,Jiangsu Key Laboratory of Biofunction Molecule,Nanjing 210013,China
关键词:
水通道蛋白; 梨花粉; ‘丰水’; ‘今村秋’
Keywords:
aquaporins; Pyrus pollen; Housui; Imanuraaki
分类号:
Q945.34
文献标志码:
A
摘要:
梨花粉在不同渗透压下萌发率会发生变化,用水通道蛋白的抑制剂HgCl2和激活剂β-巯基乙醇处理‘丰水’、‘今村秋’花粉培养基,会影响花粉萌发率.在培养基中加入浓度低于0.01%的β-巯基乙醇可以提高花粉萌发率、增加花粉管长度,若浓度高于0.01%,β-巯基乙醇即会对花粉造成毒害作用; 加入0.001 mmol·L-1 HgCl2即可降低萌发率,花粉管长度变短,且抑制效果随浓度增高而增强.
Abstract:
The pollen germination rate of Pyrus pollen tubes varied at different osmosis pressure.Under the condition of the inhibitor and activator of aquaporins(AQPs),the germination rate of Housui and Imanuraaki pollens will be affected.The concentration of AQPs activator under 0.01% will increase the germination rate and pollen tube length.Otherwise the activator will be poison to the Pyrus pollen.The pollen tube length and germination rate will be reduced when the concentration of AQPs inhibitor reach 0.001 mmol·L-1.At the same time the high level of HgCl2 will get better effects.

参考文献/References:

[1] Wang L L,Chen A P,Zhong N Q,et al.The lungiella salsuginea tonoplast aquaporin TsTIP1 functions in protection against multiple abiotic stresses[J].Plant and Cell Physiology,2014,55(1):148-161.
[2]Tzartos J S,Stergiou C,Kilidireas K,et al.Anti-aquaporin-1 autoantibodies in patients with neuromyelitis optica spectrum disorders[J].PloS One,2013,8(9):e74773.
[3]Cao X C,Zhang W R,Cao W F,et al.Aquaporin-3 is required for FGF-2-induced migration of human breast cancers[J].PloS One,2013,8(2):e56735.
[4]Maurel C,Verdoucq L,Luu D T,et al.Plant aquaporins:membrane channels with multiple integrated functions[J].Annu Rev Plant Biol,2008,59:595-624.
[5]Schuurmans J A,van Dongen J T,Rutjens B P,et al.Members of the aquaporin family in the developing pea seed coat include representatives of the PIP,TIP,and NIP subfamilies[J].Plant Mol Biol,2003,53(5):633-645.
[6]Bienert G P,Moller A L,Kristiansen K A,et al.Specific aquaporins facilitate the diffusion of hydrogen peroxide across membranes[J].J Biol Chem,2007,282(2):1 183-1 192.
[7]Liu L H,Ludewig U,Gassert B,et al.Urea transport by nitrogen-regulated tonoplast intrinsic proteins in Arabidopsis[J].Plant Physiol,2003,133(3):1 220-1 228.
[8]Holm L M,Jahn T P,Moller A L,et al.NH3 and NH+4 permeability in aquaporin-expressing Xenopus oocytes[J].Pflugers Arch,2005,450(6):415-428.
[9]Uehlein N,Lovisolo C,Siefritz F,et al.The tobacco aquaporin NtAQP1 is a membrane CO2 pore with physiological functions[J].Nature,2003,425(6959):734-737.
[10]Takano J,Wada M,Ludewig U,et al.The Arabidopsis major intrinsic protein NIP5 is essential for efficient boron uptake and plant development under boron limitation[J].Plant Cell,2006,18(6):1 498-1 509.
[11]梅杨,李海蓝,杨尚元,等.植物水孔蛋白的功能[J].植物生理学通讯,2007,43(3):563-568.
[12]李昌庆,杨致邦,夏丽君,等.渗透压对痢疾志贺菌水通道蛋白glpF基因表达的影响[J].中国微生态学杂志,2010(6):514-517.
[13]徐义流,张绍铃.梨配子体型自交不亲和性及其分子机理[J].果树学报,2003,20(1):59-63.

[14]Li G W,Peng Y H,Yu X,et al.Transport functions and expression analysis of vacuolar membrane aquaporins in response to various stresses in rice[J].J Plant Physiol,2008,165(18):1 879-1 888.
[15]徐国华,张绍铃,张超英,等.梨自花与异花授粉后花粉胞内游离Ca2+分布的变化[J].植物生理与分子生物学学报,2003,29(2):97-103.
[16]谢鹏,谭晓风,李芳东,等.6个杏李品种花粉量及花粉萌发率测定[J].中国南方果树,2009,38(2):34-35.
[17]朱美君,陈珈,王学臣.玉米根细胞质膜水孔蛋白的鉴别、分布及其功能[J].科学通报,2000(4):407-411.
[18]Yoshinori H,Noriaki O,Ikuko K,et al.Molecular mechanisms of how mercury inhibits water permeation through aquaporin-1:understanding by molecular dynamics simulation[J].Biophysical Journal,2010,98(4):1 512-1 519.

备注/Memo

备注/Memo:
收稿日期:2014-06-26.
基金项目:江苏省高校自然科学基金(11KJB210002)、江苏省大学生实践创新训练计划(201350516012Y)、江苏省高校“青蓝工程”项目.
通讯联系人:徐国华,教授,研究方向:植物生理与分子生物学.E-mail:njxgh@hotmail.com
更新日期/Last Update: 2014-12-31