[1]刘贵友,王思渊,袁　生.碳和氮源以及pH对耐有机溶剂葛根素糖基化菌株Lysinibacillus fusiformis CGMCC 4913生长及转化活性的影响[J].南京师范大学学报(自然科学版),2016,39(02):61.[doi:10.3969/j.issn.1001-4616.2016.02.012]
　Liu Guiyou,Wang Siyuan,Yuan Sheng.The Impact of Carbon，Nitrogen Source as well as pH Value on the Growth and Biotransformation Activity of Lysinibacillus fusiformisCGMCC 4913，an Organic Solvent-Tolerant Glycosylationof Puerarin Producing Strain Organic[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(02):61.[doi:10.3969/j.issn.1001-4616.2016.02.012]
点击复制

碳和氮源以及pH对耐有机溶剂葛根素糖基化菌株Lysinibacillus fusiformis CGMCC 4913生长及转化活性的影响()
分享到：

《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第39卷
期数:
2016年02期
页码:
61
栏目:
生命科学
出版日期:
2016-06-30

文章信息/Info

Title:
The Impact of Carbon，Nitrogen Source as well as pH Value on the Growth and Biotransformation Activity of Lysinibacillus fusiformisCGMCC 4913，an Organic Solvent-Tolerant Glycosylationof Puerarin Producing Strain Organic
作者:
刘贵友1; 2; 王思渊2; 袁　生2
（1.江苏第二师范学院生命科学与化学化工学院，江苏 南京 210013）（2.南京师范大学生命科学学院，江苏省微生物资源产业化工程技术研究中心，江苏省微生物与功能基因组学重点实验室，江苏 南京 210023）
Author(s):
Liu Guiyou1; 2; Wang Siyuan2; Yuan Sheng2
（1.Faculty of Life Science and Chemical Engineering，Jiangsu Second Normal University，Nanjing 210013，China）（2.School of Life Sciences，Nanjing Normal University，Jiangsu Engineering and Technology Research Center for Industrialization ofMicrobial Resources
关键词:
葛根素; 耐有机溶剂; Lysinibacillus fusiformis; 培养条件
Keywords:
puerarin; organic solvent-tolerant; Lysinibacillus fusiformis; culture conditions
分类号:
Q939.99
DOI:
10.3969/j.issn.1001-4616.2016.02.012
文献标志码:
A
摘要:
本实验从当地土壤样品中分离纯化得到一株耐有机溶剂菌株Lysinibacillus fusiformis CGMCC 4913，具有通过菌体细胞生物转化反应将葛根素转化得到葛根素-7-O-果糖苷的能力. 本研究探讨碳源、氮源以及pH等培养条件对L. fusiformis CGMCC 4913菌体生长及其转化活性的影响. 研究表明，LB培养基完全可以为L. fusiformis CGMCC 4913菌株生长提供碳源，蛋白胨和胰蛋白胨为氮源培养得到的菌株转化活性良好，在pH 7.0条件下LB培养基培养L. fusiformis CGMCC 4913菌株，菌株生长较好，有较高的转化活性且活性相对稳定.
Abstract:
An organic solvent-tolerant Lysinibacillus fusiformis CGMCC 4913，isolated from the local soil sample，has the ability to convert puerarin into puerarin-7-O-fructoside by the biotransformation. This study aimed to clarify the effects of carbon sources，nitrogen sources and pH on the growth and transforming activity of L. fusiformis CGMCC 4913. The results indicated that LB medium can totally act as carbon source for L. fusiformis CGMCC 4913. And the transformation activity of the strain taking peptone and tryptone as nitrogen source performs good. LB cultured Lysinicillus fusiformis CGMCC 4913 strain grows well and exhibits relatively high and stable transformation activity.

参考文献/References:

［1］ BENLHABIB E，BAKER J I，KEYLER D E，et al. Effect of purified puerarin on voluntary alcohol intake and alcohol withdrawal symptoms in P rats receiving free access to water and alcohol［J］. J Med Food，2004，79（2）：180-186.
［2］ 陈晓红. 266例葛根素注射液不良反应分析［J］. 中国药事，2010，24（2）：203-205.
［3］ 崔升淼，赵春顺，何仲贵. 大鼠肠管外翻模型对葛根素吸收机制的研究［J］. 时珍国医国药，2008，19（7）：1 715-1 716.
［4］ 李煦颖，张懋璠，赵妍，等. 不同剂量葛根素在大鼠体内的药代动力学［J］. 中国医科大学学报，2009，38（12）：885-887.
［5］ CHEN C C，CHAN W H. Impact effects of puerarin on mouse embryonic development［J］. Reprod Toxicol，2009，28（4）：530-535.
［6］ YUE P F，YUAN H L，ZHU W F，et al. The study to reduce the hemolysis side effect of puerarin by a submicron emulsion delivery system［J］. Biol Pharm Bull，2008，31（1）：45-50.
［7］ REN F Z，JING Q F，SHEN Y J，et al. Quantitative determination of puerarin in dog plasma by HPLC and study on the relative bioavailability of sustained release tablets［J］. J Pharm Biomed Anal，2006，41（2/3）：549-553.
［8］ WANG S Y，LIU G Y，ZHANG W，et al. Efficient glycosylation of puerarin by an organic solvent-tolerantstrain of Lysinibacillus fusiformis［J］. Enzyme Microb Tech，2014，57：42-47.
［9］ YU C G，XU H D，HUANG G D，et al. Permeabilization of Microbacterium oxydans shifts the conversion of puerarin from puerarin-7-O-glucoside to puerarin-7-O-fructoside［J］. Appl Microbiol Biotechnol，2010，86（3）：863-870.
［10］ LI D，PARK S H，SHIM J H，et al. In vitro enzymatic modification of puerarin to puerarin glycosides by maltogenic amylase［J］. Carbohyd Res，2004，339（17）：2 789-2 797.
［11］ JIANG J R，YUAN S，DING J F，et al. Conversion of puerarin into its 7-O-glycoside derivatives by Microbacterium oxydans（CGMCC 1788）to improve its water solubility and pharmacokinetic properties［J］. Appl Microbiol Biotechnol，2008，81（4）：647-657.
［12］ YE H，YUAN S，CONG X D. Biotransformation of puerarin into 3′-hydroxypuerarin by Trichoderma harzianum NJ01［J］. Enzyme Microb Tech，2007，40（4）：594-597.
［13］ LIU G Y，SUN L，WANG S Y，et al. Hydroxylation modification and free radical scavenging activity of puerarin-7-O-fructoside［J］. Folia Microbiol，2011，56（4）：305-311.
［14］ HUANG W，OCHIAI H，ZHANG X Y，et al. Introducing N-glycans into natural products through a chemoenzymatic approach［J］. Carbohydr Res，2008，343（17）：2 903-2 913.
［15］ 张连文，马晓峰，王鹏. 一种半乳糖基转移酶修饰黄酮类糖苷化合物的方法：CN101575631［P］. 2009-11-11［2015-12-15］.
［16］ CHOI C H，KIM S H，JANG J H，et al. Enzymatic synthesis of glycosylated puerarin using maltogenic amylase from Bacillus stearothermophilus expressed in Bacillus subtilis［J］. J Sci Food Agr，2010，90（7）：1 179-1 184.
［17］ LIU G Y，SUN L，WU X X，et al. Immobilization of puerarin glycosidase from Microbacterium oxydans CGMCC 1788 increases puerarin transformation efficiency［J］. Braz J Chem Eng，2014，31（2）：325-333.
［18］ DOUKYU N，YAMAGISHI W，KUWAHARA H，et al. Purification and characterization of a maltooligosaccharide-forming amylase that improves product selectivity in water-miscible organic solvents，from dimethylsulfoxide-tolerant Brachybacterium sp. strain LB25［J］. Extremophiles，2007（11）：781-788.
［19］ 何冰芳，吴薛明，储建林，等. 果糖基化葛根素及其制备方法与用途：CN102443027A［P］. 2012-05-09［2015-12-15］.
［20］ HE B，WU X，CHU J，et al. Fructosylated puerarin，and preparation method and use thereof：US8598128 B2［P］. 2013-12-03［2015-12-15］.
［21］ 何冰芳，王瑞，吴薛明，等.耐有机溶剂糖苷酶Fru6及其突变体和应用：CN102732456A［P］. 2012-10-17［2015-12-15］.
［22］ WU X M，CHU J L，WU B，et al. An efficient novel glycosylation of flavonoid by β-fructosidase resistant to hydrophilic organic solvents［J］. Bioresour Technol，2013，129：659-662.

备注/Memo

备注/Memo:
收稿日期：2015-12-19.　
基金项目：国家自然科学基金（J1103507）、江苏省微生物与功能基因组学重点实验室基金（164070303402）.　
通讯联系人：袁生，教授，研究方向：微生物代谢调控与药物合成研究、微生物生长代谢调控的分子生物学研究. E-mail：yuansheng@njnu.edu.cn
更新日期/Last Update: 2016-06-30