[1]许剑锋,朱绍汶,蒋昵真,等.江苏地区无偿献血人群HCV流行病学调查[J].南京师范大学学报(自然科学版),2016,39(02):73.[doi:10.3969/j.issn.1001-4616.2016.02.014]
 Xu Jianfeng,Zhu Shaowen,Jiang Nizhen,et al.HCV Epidemiological Survey of Voluntary Blood Donors in Jiangsu Area[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(02):73.[doi:10.3969/j.issn.1001-4616.2016.02.014]
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江苏地区无偿献血人群HCV流行病学调查()
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《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第39卷
期数:
2016年02期
页码:
73
栏目:
生命科学
出版日期:
2016-06-30

文章信息/Info

Title:
HCV Epidemiological Survey of Voluntary Blood Donors in Jiangsu Area
作者:
许剑锋朱绍汶蒋昵真胡文佳汤心怡王金花朱胜江林 红
江苏省血液中心,江苏 南京 210042
Author(s):
Xu JianfengZhu ShaowenJiang NizhenHu WenjiaTang XinyiWang JinhuaZhu ShengjiangLin Hong
Blood Center of Jiangsu Province,Nanjing 210042,China
关键词:
HCV无偿献血者基因型
Keywords:
HCVvoluntary blood donorsgenotype
分类号:
R446.1
DOI:
10.3969/j.issn.1001-4616.2016.02.014
文献标志码:
A
摘要:
本文旨在研究江苏地区无偿献血人群的HCV感染情况及其流行特征.对2013年-2014年度江苏省血液中心157,119名健康无偿献血者血液标本进行抗-HCV ELISA双试剂检测和HIV、HBV和HCV6-混样核酸检测,对HCV RNA阳性标本用定量PCR方法检测病毒滴度情况;对HCV RNA阳性标本进行巢式PCR扩增核心区片段,根据测序结果分析基因型,分析HCV基因型的分布及其与性别、年龄的关系.结果共检出347例抗-HCV反应性标本,阳性率为0.22%.经核酸检测,在抗-HCV阴性和单试剂阳性标本中均未检出HCV RNA阳性标本;125例抗-HCV双试剂阳性标本中HCV RNA阳性标本46例,病毒血症阳性率为36.8%.巢式PCR扩增HCV RNA阳性标本并成功测序和分型36例.基因型分型为1a(2.6%)、1b(74.4%)、2a(10.2%)、3b(5.1%)、6a(5.1%)和6n(2.8%)4种基因型和6种基因亚型,未发现4型和5型. 1型和2型在男性组中比例明显高于女性组,但差异无统计学意义;1型与2型患者平均年龄小于3型和6型患者,差异有统计学意义.结果显示,江苏地区无偿献血人群抗-HCV阳性率呈现下降趋势,HCV的感染与性别和职业受教育程度没有相关性,但与年龄和职业具有相关性,感染的HCV基因型以1b为主,不同基因型在不同年龄之间具有显著差异.
Abstract:
To study the prevalence of HCV infection and its epidemiological characteristics in Jiangsu area. 157,119 health blood donation of blood samples in blood center of Jiangsu Province from the year 2013 to 2014 anti HCV ELISA double reagent detection and HIV,HBV and HCV6-mixed like nucleic acid detection,the HCV RNA positive samples were detected by quantitative PCR method to determine the titer of virus;HCV RNA positive samples were nested PCR amplified fragments of the core area,according to the analysis of the gene sequencing results. Analysis of HCV genotype distribution and correlation with sex and age. A total of 347 cases of anti-HCV reactive specimens were detected,the positive rate was 0.22%. RNA HCV positive samples were not detected in the anti-HCV negative and -HCV positive samples by nucleic acid detection,and 46 cases of RNA positive samples were 125 cases of HCV positive samples,the positive rate was 36.8%. Nested PCR amplification of HCV RNA positive samples and sequencing and typing of 36 cases. Genotypes were 1a(2.6%),1B(74.4%),2A(10.2%),3b(5.1%),6a(5.1%)and 6N(2.8%)four genotypes and six kinds of genotypes,4 and 5 were not found. The proportion of type 2 and type 1 in the male group was significantly higher than that in the female group,but the difference was not statistically significant;the difference was statistically significant between the type 1,2 and type 3,6. The positive rate of anti -HCV in blood donors in Jiangsu area was decreased,and the infection of HCV was not correlated with the degree of gender and occupation education. However,there was a significant difference between genotype and age and occupation,and the genotype of HCV was 1b.

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备注/Memo

备注/Memo:
收稿日期:2015-05-28. 
基金项目:“六大人才高峰”第十一批高层次人才选拔培养资助项目(WSN-002)、江苏省卫生厅科技项目(H20140025)、江苏省自然科学基金(BK20131440). 
通讯联系人:林红,研究员,研究方向:输出相关传染病与血液安全研究. E-mail:linhong712003@sina.com
更新日期/Last Update: 2016-06-30