[1]沈佳斌,张雪敬,吴 蔚,等.雉科鸟类肠道微生物的多样性与功能及其对食性的适应[J].南京师范大学学报(自然科学版),2016,39(02):90.[doi:10.3969/j.issn.1001-4616.2016.02.017]
 Shen Jiabin,Zhang Xuejing,Wu Wei,et al.The Gut Microbes’ Diversity and Function of PhasianidaeProvide Insights on the Adaptation to Their Diet[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(02):90.[doi:10.3969/j.issn.1001-4616.2016.02.017]
点击复制

雉科鸟类肠道微生物的多样性与功能及其对食性的适应()
分享到:

《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第39卷
期数:
2016年02期
页码:
90
栏目:
生命科学
出版日期:
2016-06-30

文章信息/Info

Title:
The Gut Microbes’ Diversity and Function of PhasianidaeProvide Insights on the Adaptation to Their Diet
作者:
沈佳斌1张雪敬1吴 蔚1胡婉婷1张成林2朱立峰1
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.北京动物园,北京 100000)
Author(s):
Shen Jiabin1Zhang Xuejing1Wu Wei1Hu Wanting1Zhang Chenglin2Zhu Lifeng1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.Beijing Zoo,Beijing 100000)
关键词:
肠道菌群雉科功能
Keywords:
gut microbiotaPhasianidaefunction
分类号:
Q958;Q938
DOI:
10.3969/j.issn.1001-4616.2016.02.017
文献标志码:
A
摘要:
肠道菌群对动物的生命活动有着极其重要的影响. 本研究利用高通量测序手段,对雉科(Phasianidae)鸟类粪便进行16S rRNA基因测序,并结合其自身的杂食性饮食结构,探究了雉科鸟类肠道菌群的情况,共得到189 860条有效序列并定义4 305个OTU(Operational Taxonomic Unit). 结果发现雉科鸟类肠道菌群中变形菌门的相对丰度高于许多已知的其他鸟类. 尽管食性相同,但雉科鸟类肠道菌群依然存在部分分化的现象,说明宿主系统发生地位对肠道菌群结构起到了重要的作用. 不仅如此,更深入的宏基因组研究显示,雉科肠道菌群功能中,分解雉科摄入的有害物质的通路被富集,以此来确保雉科鸟类的正常生命活动.
Abstract:
Intestinal flora plays an important role on the life activities of animals. In this study,high throughput 16S rRNA gene sequencing method on feces was used to investigate the intestinal flora of Phasianidae birds considering their omnivorous diet structure. The study totally gained 189 860 sequences and defined 4 305 OTUs. It was found that the gut microbes of Phasianidae birds had relative high proportion of Proteobacteria compared with those from other birds. Although treated with the same diet,it was still found the partial divergence on their gut microbes,which reflecting indicating that the status of host phylogeny plays an important role in shaping the gut microbiome. Further metagenomics analysis revealed gut microbes of Phasianidae animals were potential enriched in these metabolism pathway on many harmful substances intaken,which ensures the normal life activities of the Phasianidae.

参考文献/References:

[1] QUIGLEY E M M. Prebiotics and probiotics;modifying and mining the microbiota[J]. Pharmacological research,2010,61(3):213-218.
[2] LEY R E,HAMADY M,LOZUPONE C,et al. Evolution of mammals and their gut microbes[J]. Science,2008,320(5883):1647-1651.
[3] DAVID L A,MAURICE C F,CARMODY R N,et al. Diet rapidly and reproducibly alters the human gut microbiome[J]. Nature,2014,505(7 484):559-563.
[4] ZHU L,WU Q,DAI J,et al. Evidence of cellulose metabolism by the giant panda gut microbiome[J]. Proceedings of the national academy of sciences,2011,108(43):17 714-17 719.
[5] ROGGENBUCK M,SCHNELL I B,BLOM N,et al. The microbiome of New World vultures[J]. Nature communications,2014,18:5.
[6] GODOY V F,GOLDFARB K C,KARAOZ U,et al. Comparative analyses of foregut and hindgut bacterial communities in hoatzins and cows[J]. The ISME Journal,2012,6(3):531-541.
[7] ECKBURG P B,BIK E M,BERNSTEIN C N,et al. Diversity of the human intestinal microbial flora[J]. Science,2005,308(5?728):1 635-1 638.
[8] 许波,杨云娟,李俊俊,等. 宏基因组学在人和动物胃肠道微生物研究中的应用进展[J]. Chinese journal of biotechnology,2013,29(12):1 721-1 735.
[9] ZHU X Y,ZHONG T,PANDYA Y,et al. 16S rRNA-based analysis of microbiota from the cecum of broiler chickens[J]. Applied and environmental microbiology,2002,68(1):124-137.
[10] DANZEISEN J L,CALVERT A J,NOLL S L,et al. Succession of the turkey gastrointestinal bacterial microbiome related to weight gain[J]. Peer J,2013(1):e237.
[11] 马纲,张敏. 中国雉科鸟类特有种分布的研究[J]. 西南民族大学学报(自然科学版),2009,35(3):495-499.
[12] 张正旺,丁长青,丁平,等. 中国鸡形目鸟类的现状与保护对策[J]. 生物多样性,2003,11(5):414-421.
[13] CAPORASO J G,KUCZYNSKI J,STOMBAUGH J,et al. QIIME allows analysis of high-throughput community sequencing data[J]. Nature methods,2010,7(5):335-336.
[14] SAITO R,SMOOT M E,ONO K,et al. A travel guide to Cytoscape plugins[J]. Nature methods,2012,9(11):1 069-1 076.
[15] LANGILLE M G I,ZANEVELD J,CAPORASO J G,et al. Predictive functional profiling of microbial communities using 16S rRNA marker gene sequences[J]. Nature biotechnology,2013,31(9):814-821.
[16] KERSTERS K,de VOS P,GILLIS M,et al. Introduction to the proteobacteria[M]//The prokaryotes. New York:Springer,2006:3-37.
[17] BANKS J C,CARY S C,HOGG I D. The phylogeography of Adelie penguin faecal flora[J]. Environmental microbiology,2009,11(3):577-588.
[18] BENSKIN C M W,RHODES G,PICKUP R W,et al. Diversity and temporal stability of bacterial communities in a model passerine bird,the zebra finch[J]. Molecular ecology,2010,19(24):5 531-5 544.
[19] COYTE K Z,SCHLUTER J,FOSTER K R. The ecology of the microbiome:networks,competition,and stability[J]. Science,2015,350(6 261):663-666.
[20] HUANG Z,LIU N,XIAO Y,et al. Phylogenetic relationships of four endemic genera of the Phasianidae in China based on mitochondrial DNA control-region genes[J]. Molecular phylogenetics and evolution,2009,53(2):378-383.

备注/Memo

备注/Memo:
收稿日期:2016-05-25. 
基金项目:国家自然科学基金(31222009). 
通讯联系人:朱立峰,博士,教授,研究方向:动物生态学. E-mail:zhulf@ioz.ac.cn
更新日期/Last Update: 2016-06-30