[1]康 丹,吴朝蒙,曹润怿,等.根皮素引起前列腺癌LNCaP细胞发生凋亡的机制研究[J].南京师范大学学报(自然科学版),2017,40(02):43.[doi:10.3969/j.issn.1001-4616.2017.02.008]
 Kang Dan,Wu Zhaomeng,Cao Runyi,et al.Molecular Mechanism of Phloretin-Induced Apoptosisin Prostate Cancer LNCaP Cells[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(02):43.[doi:10.3969/j.issn.1001-4616.2017.02.008]
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根皮素引起前列腺癌LNCaP细胞发生凋亡的机制研究()
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《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年02期
页码:
43
栏目:
·生命科学·
出版日期:
2017-06-29

文章信息/Info

Title:
Molecular Mechanism of Phloretin-Induced Apoptosisin Prostate Cancer LNCaP Cells
文章编号:
1001-4616(2017)02-0043-08
作者:
康 丹吴朝蒙曹润怿王方方田 倩刘 平
南京师范大学生命科学学院,江苏省分子与医学生物技术重点实验室,江苏 南京 210023
Author(s):
Kang DanWu ZhaomengCao RunyiWang FangfangTian QianLiu Ping
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Molecular and Medical Biotechnology,Nanjing 210023,China
关键词:
根皮素细胞凋亡细胞周期PI3K/AKT信号通路前列腺癌
Keywords:
phloretincell apoptosiscell cyclePI3K/AKT signal pathwayprostate cancer
分类号:
Q291
DOI:
10.3969/j.issn.1001-4616.2017.02.008
文献标志码:
A
摘要:
根皮素是存在于苹果、梨等水果及多种蔬菜汁液中的天然活性物质,具有抗肿瘤活性,能够有效地诱导细胞凋亡、抑制细胞增殖. 本研究初步探讨了根皮素诱导前列腺癌LNCaP细胞的凋亡和相关分子机制. 通过形态学观察、噻唑蓝(MTT)比色实验、CCK-8实验测定前列腺癌细胞活性; 采用DAPI染色法测定细胞核凝缩破裂; 运用Annexin V-FITC/PI双染流式细胞术测定前列腺癌细胞的凋亡率; 再通过PI单染流式细胞术测定前列腺癌细胞的周期变化; 以及采用Western blot检测PI3K/AKT信号通路及其下游细胞凋亡相关蛋白和细胞周期相关蛋白的表达. 实验结果表明:根皮素诱导前列腺癌LNCaP细胞周期阻滞在G2/M期,并呈浓度依赖性降低细胞活性、增加细胞核凝缩破裂,显著提高前列腺癌细胞的凋亡率. 在分子机制上,根皮素呈现浓度依赖性下调PI3K/AKT的活性,从而影响细胞凋亡相关蛋白和细胞周期相关蛋白的表达. 因此,根皮素对前列腺癌细胞的生长有显著的抑制作用,对治疗前列腺癌具有潜在的临床应用价值.
Abstract:
Phloretin,a natural product found in pears,apples and various vegetables,exerts anti-inflammatory,anti-oxidant and anti-tumor effects. Here,we investigated the molecular mechanism of phloretin-induced apoptosis in prostate cancer LNCaP cells. In this study,effects of phloretin on the viability of prostate cancer cells were investigated by morphological observation,MTT assay and CCK-8 assay,respectively. Effect of phloretin on apoptosis and cell cycle was measured by Flow Cytometry. Furthermore,western blot was employed to investigate the levels of phosphorylation of PI3K and AKT(the activity of PI3K/AKT signaling pathway); and check the expression of cell apoptosis-related and cell cycle-related proteins which were downstream of PI3K/AKT signaling pathway in LNCaP cells. As a result,we found that phloretin significantly blocked cell cycle arrest in G2/M phase by decreasing the protein level of Cyclin B1,and decreased the cell viability and increased the nuclear rupture in a dose-dependent manner; and finally resulted in a significantly apoptosis in LNCaP cells. In molecular level,phloretin showed a dose-dependent down-regulation of the activity of PI3K/AKT via down-regulating the phosphorylation of PI3K and AKT proteins,and then regulated the expression of cell apoptosis-related and cell cycle-related proteins. Therefore,phloretin significantly inhibited cell viability and promoted apoptosis in prostate cancer cells,suggesting that phloretin had potential clinical value for treatment of prostate cancer in clinic.

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备注/Memo

备注/Memo:
收稿日期:2016-10-15.
基金项目:国家自然科学基金(81272850、81472415).
通讯联系人:刘平,教授,博士生导师,研究方向:肿瘤发生与发展的分子机制. E-mail:08201@njnu.edu.cn
更新日期/Last Update: 2017-06-30