[1]齐雅玲,卢悟广,种丹阳,等.蛋白质异戊二烯化关键酶GGPPS结合小分子筛选模型的构建[J].南京师范大学学报(自然科学版),2017,40(04):87.[doi:10.3969/j.issn.1001-4616.2017.04.014]
 Qi Yaling,Lu Wuguang,Chong Danyang,et al.The Construction of Mevalonate Pathway Enzyme GGPPSProtein Binding Molecules Screening Model[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(04):87.[doi:10.3969/j.issn.1001-4616.2017.04.014]
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蛋白质异戊二烯化关键酶GGPPS结合小分子筛选模型的构建()
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《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年04期
页码:
87
栏目:
·生命科学·
出版日期:
2017-12-30

文章信息/Info

Title:
The Construction of Mevalonate Pathway Enzyme GGPPSProtein Binding Molecules Screening Model
文章编号:
1001-4616(2017)04-0087-06
作者:
齐雅玲1卢悟广2种丹阳1刘 佳1孙 倩1徐晓军3曹 鹏2方 雷1李朝军1薛 斌1
(1.南京大学医学院,医药生物技术国家重点实验室,江苏省医学分子技术重点实验室,江苏 南京 210093)(2.江苏省中西医结合医院,江苏 南京 210028)(3.中国药科大学,天然药物活性组分与药效国家重点实验室,江苏 南京 210009)
Author(s):
Qi Yaling1Lu Wuguang2Chong Danyang1Liu Jia1Sun Qian1Xu Xiaojun3Cao Peng2Fang Lei1Li Chaojun1Xue Bin1
(1.State Key Laboratory of Pharmaceutical Biotechnology and Jiangsu Key Laboratory of Molecular Medicine,School of Medicine,Nanjing University,Nanjing 210093,China)(2.Jiangsu Province Hospital on Integration of Chinese and Western Medicine,Nanjing 21002
关键词:
GGPPS重组蛋白微量热泳动小分子筛选
Keywords:
GGPPS recombination proteinmicroscale thermophoresissmall molecular screening
分类号:
Q291
DOI:
10.3969/j.issn.1001-4616.2017.04.014
文献标志码:
A
摘要:
蛋白质的基本翻译后修饰包括磷酸化、糖基化、乙酰化、棕榈酸化等[1],而异戊二烯化修饰是蛋白质的基本翻译后修饰之一,属于蛋白质的脂质修饰[2]. 甲羟戊酸途径中的关键分支酶——香叶基香叶基焦磷酸合成酶(Geranylgeranyl diphosphate synthase,GGPPS)能够催化法尼基焦磷酸盐(Farnesyl diphosphate,FPP)生成香叶基香叶基焦磷酸盐(Geranylgeranyl diphosphate,GGPP),介导蛋白质异戊二
Abstract:
The post-translational modifications of proteins include phosphorylation,glycosylation,acetylation,palmitic acid and so on. And the prenylation modification of proteins is one of the basic post-translational lipid modifications of proteins. The key enzyme of mevalonate pathway,GGPPS,can catalyze FPP to generate GGPP and mediate the equilibrium of protein prenylation,affect disease progression including insulin resistance,hyperyension,abnormal reproductive metabolism. Hence,it has an important practical significance that construct a GGPPS interaction model to obtain its molecule therapeutic drugs. Obtain the high expression of EGFP fluorescent labeled GGPPS prokaryotic purified protein. Construct a GGPPS interaction model with microscale thermophoresis technology. The recombinant expression plasmid GGPPS-EGFP-His was constructed correctly verified by gel electrophoresis and sequencing verification.The purified recombinant protein reached a purity of about 80%. In the microscale thermophoresis assay,the GGPPS substrate farnesyl pyrophosphate concentration gradient test showed a good combination.

参考文献/References:

[1] KONSTANTINOPOULOS P A,KARAMOUZIS M V,PAPAVASSILIOU A G. Post-translational modifications and regulation of the RAS superfamily of GTPases as anticancer targets[J]. Nat Rev Drug Discov,2007,6(7):541-555.
[2]BERNDT N,HAMILTON A D,SEBTI S M. Targeting protein prenylation for cancer therapy[J]. Nat Rev Cancer,2011,11(11):775-791.
[3]MARSHALL C. Protein prenylation:a mediator of protein-protein interactions[J]. Science,1993,259(5 103):1 865-1 867.
[4]SHEN N,YU X,PAN F Y,et al. An early response transcription factor,Egr-1,enhances insulin resistance in type 2 diabetes with chronic hyperinsulinism[J]. J Biol Chem,2011,286(16):14 508-14 515.
[5]JIANG S,SHEN D,JIA W J,et al. GGPPS-mediated Rab27A geranylgeranylation regulates β cell dysfunction during type 2 diabetes development by affecting insulin granule docked pool formation[J]. The journal of pathology,2016,238(1):109-119.
[6]WANG X X,YING P,DIAO F,et al. Altered protein prenylation in Sertoli cells is associated with adult infertility resulting from childhood mumps infection[J]. Journal of experimental medicine,2013,210(8):1 559-1 574.
[7]王孝平,邢树礼. 考马斯亮蓝法测定蛋白含量的研究[J]. 天津化工,2009(3):40-42.
[8]蒋世强,潘能庆,鄢航,等. 重组蛋白纯化研究进展[J]. 现代农业科技,2017(4):250-251,254.
[9]JERABEK W M,WIENKEN C J,BRAUN D,et al. Molecular interaction studies using microscale thermophoresis[J]. Assay and drug development technologies,2011,9(4):342-353.
[10]GISSELBERG J E,ZHANG L,ELIAS J E,et al. The prenylated proteome of Plasmodium falciparum reveals pathogen-specific prenylation activity and drug mechanism-of-action[J]. Molecular and cellular proteomics,2017,16(4 suppl 1):S54-S64.
[11]SHEN N,JIANG S,LU J M,et al. The constitutive activation of Egr-1/C/EBPa mediates the development of type 2 diabetes mellitus by enhancing hepatic gluconeogenesis[J]. The American journal of pathology,2015,185(2):513-523.
[12]TAO W,WU J,XIE B X,et al. Lipid-induced muscle insulin resistance is mediated by GGPPS via modulation of the RhoA/Rho kinase signaling pathway[J]. Journal of biological chemistry,2015,290(33):20 086-20 097.
[13]BAJUSZ D,FERENCZY G G,KESERU G M. Structure-based virtual screening approaches in kinase-directed drug discovery[J]. Current topics in medicinal chemistry,2017,17(20):2 235-2 259.
[14]COHEN K E. System and method for drug screening and monitoring pupil reactivity and voluntary and involuntary eye muscle function[J]. Google patents,2016.
[15]BREITSPRECHER D,SCHLINCK N,WITTE D,et al. Aptamer binding studies using micro scale thermophoresis[J]. Nucleic acid aptamers:selection,characterization,and application,2016:99-111. DOI:10.1007/978-1-4939-3197-2-8.
[16]ENTZIAN C,SCHUBERT T. Studying small molecule-aptamer interactions using MicroScale Thermophoresis(MST)[J]. Methods,2016,97:27-34.
[17]SEIDEL S A,WIENKEN C J,GEISSLER S,et al. Label-free microscale thermophoresis discriminates sites and affinity of protein-ligand binding[J]. Angewandte chemie international edition,2012,51(42):10 656-10 659.
[18]KHAVRUTSKII L,YEH J,TIMDFEEVA O,et al. Protein purification-free method of binding affinity determination by microscale thermophoresis[J]. Journal of visualized experiments,2013(78):e50541-e50541.
[19]JERABEK W M,ANORE T,WANNER R,et al. MicroScale thermophoresis:interaction analysis and beyond[J]. Journal of molecular structure,2014,1077:101-113.
[20]CARRARA M,PRISCHI F,NOWAK P R,et al. Noncanonical binding of BiP ATPase domain to Ire1 and Perk is dissociated by unfolded protein CH1 to initiate ER stress signaling[J]. Elife,2015(4):e03522.

备注/Memo

备注/Memo:
收稿日期:2016-06-24.
基金项目:国家自然科学基金(31371373)、江苏省自然科学基金(BK20151395)、天然药物活性组分与药效国家重点实验室开放基金(SKLNMKF201606)、中央高校基本科研业务费专项资金(021414380330).
通讯联系人:薛斌,博士,副教授,研究方向:代谢类疾病的调控机制、肝损伤再生恢复研究. E-mail:xuebin@nju.edu.cn
更新日期/Last Update: 2017-12-30