Li Haimei,Wang Yingying,Gao Tao,et al.FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum[J].Journal of Nanjing Normal University(Natural Science Edition),2018,41(01):76.[doi:10.3969/j.issn.1001-4616.2018.01.014]





FgPDK1 Modulates Osmotic Stress Responses in Fusarium graminearum
(1.南京师范大学生命科学学院,江苏 南京 210023)(2.江苏省农业科学院农产品质量安全与营养研究所,江苏 南京 210014)(3.江苏省农业科学院中心实验室,江苏 南京 210014)
Li Haimei1Wang Yingying1Gao Tao2Xu Cunfa3Shi Zhiqi2Chen Jian2Xu Xiaofeng1
(1.School of Life Sciences,Nanjing Normal University,Nanjing 210023,China)(2.Institute of Food Safety and Nutrition,Jiangsu Academy of Agriculture Science,Nanjing 210014,China)(3. Central Laboratory,Jiangsu Academy of Agriculture Science,Nanjing 210014,
Fusarium graminearumPDKosmotic stressglycerolcell death
真菌为了应对渗透胁迫进化出包括高渗甘油途径(high osmolarity glycerol,HOG)在内的一系列调控网络,但其上游信号调控因子尚不明确. 本文以禾谷镰孢菌(Fusarium graminearum)为研究对象,在盐胁迫下通过遗传学和生理生化手段,研究了一个关键的线粒体能量代谢酶基因丙酮酸脱氢酶激酶(FgPDK1)在调控渗透胁迫方面的重要作用. 结果显示,(1)菌丝生长测定结果表明FgPDK1敲除突变体(ΔFgPDK1)对盐胁迫的敏感性较野生型(PH-1)显著增加;(2)与PH-1相比较,
Fungi’s HOG(high osmolarity glycerol)pathway in response to osmotic stress has been revealed. However,the upstream signaling regulator of the pathway remains unclear. In this study,we investigated the pivotal role of a mitochondrial energy metabolic gene encoding for pyruvate dehydrogenase kinase(FgPDK1)in Fusarium graminearum in the regulation of NaCl stress by using genetic and physiological-biochemical approaches. The main results have been obtained as follows,(1)Determination of hyphal growth indicated that ΔFgPDK1(an FgPDK1 knock-out mutant)was more sensitive than wild type(PH-1).(2)Compared to PH-1,NaCl stress induced more electrolyte leakage and higher accumulation of glycerol in the hyphae of ΔFgPDK1.(3)ROS accumulation,MDA content,and cell death increased significantly in ΔFgPDK1 as compared to PH-1 under NaCl stress.(4)The relative expression levels of anti-oxidative genes(SOD and CAT)were significantly lower in ΔFgPDK1 as compared to PH-1 under NaCl stress.(5)All of the above physiological and biochemical responses could be recovered to the level of PH-1 in ΔFgPDK1-C(a gain-of-function complementary strain of ΔFgPDK1). These results suggested that FgPDK1 played important roles in the regulation of HOG downstream pathway and the maintenance of anti-oxidative capacity in F.graminearum under high osmatic stress. This study provides new evidence for understanding the molecular mechanism in fungi’s osmatic stress adaption.


[1] 何家泌. 小麦赤霉病菌源种类和禾谷镰刀菌的特性及变异研究综述[J]. 国外农学:植物保护,1992(4):9-11.
[2]MESTERHAZY A. Types and components of resistance to Fusarium head blight of wheat[J]. Plant breeding,1995,114(5):377-386.
[3]PARRY D W,JENKINSON P,MCLEOD L. Fusarium head blight(scab)in small grain cereals—a review[J]. Plant pathology,1995,44(2):207-238.
[4]CROMEY M G,SHORTER S C,LAUREN D R,et al. Cultivar and crop management influences on Fusarium head blight and mycotoxins in spring wheat(Triticum aestivum)in New Zealand[J]. New Zealand journal of crop and horticultural science,2002,30(4):235-247.
[5]陈利锋,徐敬友. 农业植物病理学[M]. 北京:中国农业出版社,2007.
[6]WONG P,WALTER M,LEE W,et al. FGDB:revisiting the genome annotation of the plant pathogen Fusarium graminearum[J]. Nucleic acids research,2011,39:D637-D639.
[7]ROCIO D,JEFFREY W C,ANA M C. Role of the osmotic stress regulatory pathway in morphogenesis and secondary metabolism in Filamentous Fungi[J]. Toxins,2010,2,367-368.
[8]SAXENA A,SITARAMAN R. Osmoregulation in Saccharomyces cerevisiae via mechanisms other than the high-osmolarity glycerol pathway[J]. Microbiology,2016,162:1 511-1 526.
[9]PRONK J T,YDE STEENSMA H,van DIJKEN J P. Pyruvate metabolism in Saccharomyces cerevisiae[J]. Yeast,1996,12(16):1 607-1 633.
[10]HURD T R,COLLINS Y,ABAKUMOVA I,et al. Inactivation of pyruvate dehydrogenase kinase 2 by mitochondrial reactive oxygen species[J]. Journal of biological chemistry,2012,287(42):35 153-35 160.
[11]PIAO L,SIDHU V K,FANG Y H,et al. FOXO1-mediated upregulation of pyruvate dehydrogenase kinase-4(PDK4)decreases glucose oxidation and impairs right ventricular function in pulmonary hypertension:therapeutic benefits of dichloroacetate[J]. Journal of molecular medicine,2013,91(3):333-346.
[12]PEREIRA R R,CASTANHEIRA D,TEIXEIRA J A,et al. Detailed search for protein kinase(s)involved in plasma membrane H+-ATPase activity regulation of yeast cells[J]. FEMS Yeast Research,2015,15(2):fov003.
[13]ZHANG S,HULVER M W,MCMILLAN R P,et al. The pivotal role of pyruvate dehydrogenase kinases in metabolic flexibility[J]. Nutrition and metabolism,2014,11:10.
[14]GEY U,CZUPALLA C,HOFLACK B,et al. Yeast pyruvate dehydrogenase complex is regulated by a concerted activity of two kinases and two phosphatases[J]. Journal of biological chemistry,2008,283(15):9 759-9 767.
[15]YDE S H,TOMASKA L,REUVEN P,et al. Disruption of genes encoding pyruvate dehydrogenase kinases leads to retarded growth on acetate and ethanol in Saccharomyces cerevisiae[J]. Yeast,2008,25(1):9-19.
[16]GAO T,CHEN J,SHI Z. Fusarium graminearum pyruvate dehydrogenase kinase 1(FgPDK1)is critical for conidiation,mycelium growth,and pathogenicity[J]. PLoS ONE,2016,11:e0158077.
[17]PASTOR M M,PROFT M,PASCUAL A A. Mitochondrial function is an inducible determinant of osmotic stress adaptation in yeast[J]. Journal of biological chemistry,2009,284:30 307-30 317.
[18]段亚冰. 咯菌腈(适乐时)对油菜菌核病菌(Sclerotinia sclerotiorum)的作用机制研究[D]. 南京:南京农业大学,2013.
[19]YANG Y,FAN F,ZHUO R,et al. Expression of the laccase gene from a white rot fungus in Pichia pastoris can enhance the resistance of this yeast to H2O2-mediated oxidative stress by stimulating the glutathione-based antioxidative system[J]. Appl Environ Microbiol,2012,78:5 845-5 854.
[20]YAN J,QIU T. Determination of glycerol by cupric glycerinate colorimetry[J]. China oils and fats,2004,1:010.
[21]FOREMAN J,DEMIDCHIK V,BOTHWELL J H F,et al. Reactive oxygen species produced by NADPH oxidase regulate plant cell growth[J]. Nature,2003,422(6 930):442-446.
[22]KELLERMEIER F,CHARDON F,AMTMANN A. Natural variation of Arabidopsis root architecture reveals complementing adaptive strategies to potassium starvation[J]. Plant physiology,2013,161(3):1 421-1 432.
[23]WOJDA. Response to high osmotic conditions and elevated temperature in Saccharomyces cerevisiae is controlled by intracellular glycerol and involves coordinate activity of MAP kinase pathways[J]. Microbiology,2003,149(5):1 193-1 194.
[24]祝文婷,陈为京,陈建爱,等. 丛枝菌根真菌提高植物抗盐碱胁迫能力的研究进展[J]. 安徽农业科学,2013,41(5):2 061-2 062.
[25]ALTINTAS A,MARTINI J,MORTENSEN U H,et al. Quantification of oxidative stress phenotypes based on high-throughput growth profiling of protein kinase and phosphatase knockouts[J]. FEMS Yeast Research,2016(16):fov101.
[26]BOLISETTY S,JAIMES E A. Mitochondria and reactive oxygen species:physiology and pathophysiology[J]. International journal of molecular sciences,2013,14:6 306-6 344.
[27]NEWINGTON J T,RAPPON T,ALBERS S,et al. Overexpression of pyruvate dehydrogenase kinase 1 and lactate dehydrogenase A in nerve cells confers resistance to amyloid beta and other toxins by decreasing mitochondrial respiration and reactive oxygen species production[J]. J Biol Chem,2012,287(44):37 245-37 258.
[28]ABHISHEK S,RAMAKRISHNAN S. Osmoregulation in Saccharomyces cerevisiae via mechanisms other than the high-osmolarity glycerol pathway[J]. Microbiology,2016,162:1 511-1 513.
[29]PEREIRA R R,CASTANHEIRA D,TEIXEIRA J A,et al. Detailed search for protein kinase(s)involved in plasma membrane H+-ATPase activity regulation of yeast cells[J]. FEMS Yeast Research, 2015,15:15-19.
[30]PORTILLO F. Regulation of plasma membrane H(+)-ATPase in fungi and plants[J]. Biochimica et biophysica acta(BBA)-revtews on biomembranes,2000(1 469):31-42.


 Lin Ling,Shi Jianrong,Chen Huaigu,et al.Preparation and Regeneration of Proplasts for Fusarium garaminearum Schwabe[J].Journal of Nanjing Normal University(Natural Science Edition),2001,24(01):88.


通讯联系人:许晓风,教授,博导,研究方向:生理生化调控以及分子遗传学. E-mail:xuxiaofeng@njnu.edu.cn
更新日期/Last Update: 2018-03-31