[1]抗晶晶,刘晓宁.线粒体ROS改变参与石蒜碱诱导的HepG2细胞凋亡[J].南京师范大学学报(自然科学版),2019,42(04):91-96.[doi:10.3969/j.issn.1001-4616.2019.04.013]
 Kang Jingjing,Liu Xiaoning.Mitochondrial ROS Contributes to Lycorine-inducedApoptosis in HepG2 Cells[J].Journal of Nanjing Normal University(Natural Science Edition),2019,42(04):91-96.[doi:10.3969/j.issn.1001-4616.2019.04.013]
点击复制

线粒体ROS改变参与石蒜碱诱导的HepG2细胞凋亡()
分享到:

《南京师范大学学报》(自然科学版)[ISSN:1001-4616/CN:32-1239/N]

卷:
第42卷
期数:
2019年04期
页码:
91-96
栏目:
·生命科学·
出版日期:
2019-12-30

文章信息/Info

Title:
Mitochondrial ROS Contributes to Lycorine-inducedApoptosis in HepG2 Cells
文章编号:
1001-4616(2019)04-0091-06
作者:
抗晶晶刘晓宁
黄河科技学院医学院生化教研室,河南 郑州 450063
Author(s):
Kang JingjingLiu Xiaoning
Department of Biochemistry,School of Medicine,Huanghe Science and Technology College,Zhengzhou 450063,China
关键词:
石蒜碱人类肝癌HepG2细胞细胞凋亡mito-Grx1-roGFP2线粒体ROSSS31
Keywords:
Lycorinehuman hepatoma HepG2 cellsapoptosismito-Grx1-roGFP2mitochondrial ROSSS31
分类号:
R392.5
DOI:
10.3969/j.issn.1001-4616.2019.04.013
文献标志码:
A
摘要:
探究石蒜碱诱导HepG2细胞凋亡的分子机理. CCK-8实验检测细胞增殖情况; 流式细胞术检测加或不加SS31情况下,石蒜碱对HepG2细胞凋亡比例的影响; 用核酸染料Hoechst 33342评估凋亡细胞细胞核的形态变化; 用Western blot分析技术检测PARP片段的表达情况. 成功构建靶向线粒体的Mito-Grx1-roGFP2转基因稳定株肝癌细胞系HepG2. SS31作为线粒体ROS的定向清除剂,能够改善石蒜碱引起的HepG2细胞形态学损伤及细胞核内PARP切割,抑制石蒜碱诱导的细胞凋亡,
Abstract:
To further explore the underlying apoptotic molecular mechanism of lycorine on HepG2 cells. The growth of HepG2 cells were measured with TransDetect Cell Counting Kit(CCK-8)assay. Apoptosis percentages of cells exposed to lycorine with or without SS31 was determined by flow cytometry. Apoptotic nuclear morphology was assessed using Hoechst 33342. Western blot analysis was used to examine the expression of fragmented PARP. It was successfully established that an human hepatoma cell line HepG2 with mito-Grx1-roGFP2 probe stably expressed which could be used as a real-time reporter of dynamic redox changes. As an antioxidant which could remove the ROS of mitochondria,the treatment of SS31 improved the morphology of HepG2 cells injured by lycorine and inhibited lycorine-induced cell apoptosis. It was also found that SS31 significantly inhibited the fragment of PARP induced by lycorine. Furthermore,SS31 attenuated lycorine-induced rise of 405/488 fluorescence ratio which could reflect the level of ROS in mitochondria. The findings suggest that mitochondrial ROS contribute to lycorine-induced apoptosis in HepG2 cells and lycorine is expected to be a potential drug for the treatment of liver cancer,it worth profund study.

参考文献/References:

[1] SENER B,ORHAN I,SATAYAVIVAD J. Antimalarial activity screening of some alkaloids and the plant extracts from Amaryllidaceae[J]. Phytother Res,2003,17(10):1220-1223.
[2]MIKAMI M,KITAHARA M,KITANO M,et al. Suppressive activity of lycoricidinol(narciclasine)against cytotoxicity of neutrophil-derived calprotectin,and its suppressive effect on rat adjuvant arthritis model[J]. Biol Pharm Bull,1999,22(7):674-678.
[3]KANG J,ZHANG Y,CAO X,et al. Lycorine inhibits lipopolysaccharide-induced iNOS and COX-2 up-regulation in RAW264.7 cells through suppressing P38 and STATs activation and increases the survival rate of mice after LPS challenge[J]. Int Immunopharmacol,2012,12(1):249-256.
[4]LIU X S,JIANG J,JIAO X Y,et al. Lycorine induces apoptosis and down-regulation of Mcl-1 in human leukemia cells[J]. Cancer Lett,2009,274(1):16-24.
[5]LIU J,HU W X,HE L F,et al. Effects of lycorine on HL-60 cells via arresting cell cycle and inducing apoptosis[J]. Febs Lett,2004,578(3):245-250.
[6]LI Y,LIU J,TANG L J,et al. Apoptosis induced by lycorine in KM3 cells is associated with the G0/G1 cell cycle arrest[J]. Oncol Rep,2007,17(2):377-384.
[7]刘晓宁,薄惠,刘翠娥. 靶向线粒体的mt-roGFP2荧光探针检测人肝癌HepG2细胞线粒体ROS水平动态变化[J]. 山东医药,2018,58(8):14-17.
[8]BARATA A G,DICK T P. In vivo imaging of H2O2 production in Drosophila.[J]. Methods enzymol,2013,526:61-82.
[9]LI G,KANG J,YAO X,et al. The component of green tea,L-theanine protects human hepatic L02 cells from hydrogen peroxide-induced apoptosis[J]. Eur Food Res Technol,2011,233(3):427-435.
[10]LI J,CHEN X,XIAO W,et al. Mitochondria-targeted antioxidant peptide SS31 attenuates high glucose-induced injury on human retinal endothelial cells[J]. Biochem Bioph Res Co,2011,404(1):349-356.
[11]GADDUCCI A,GUERRIERI M E. PARP inhibitors in epithelial ovarian cancer:state of art and perspectives of clinical research[J]. Anticancer research,2016,36(5):2055-2064.
[12]LOPERGOLO A,PENNATI M,GANDELLINI P,et al. Apollon gene silencing induces apoptosis in breast cancer cells through p53 stabilisation and caspase-3 activation[J]. Brit J Cancer,2016,100(5):739-746.
[13]SCHAAR C E,DUES D J,SPIELBAUER K K,et al. Mitochondrial and cytoplasmic ROS have opposing effects on lifespan[J]. Plos Genet,2015,11(2):1-24.
[14]WOLIN M S,AHMAD M,GUPTE S A. Oxidant and redox signaling in vascular oxygen sensing mechanisms:basic concepts,current controversies,and potential importance of cytosolic NADPH[J]. Am J Physiol-lung C,2005,289(2):159-173.
[15]MüLLER A,SCHNEIDER J F,DEGROSSOLI A,et al. Systematic in vitro assessment of responses of roGFP2-based probes to physiologically relevant oxidant species[J]. Free radical biology and medicine,2017,106:329-338.
[16]李居伟,杨扬. 石蒜碱药理作用的研究进展[J]. 中国保健营养,2017,27(6):28-29.
[17]YU H,QIU Y,PANG X,et al. Lycorine promotes autophagy and apoptosis via TCRP1/Akt/mTOR axis inactivation in human hepatocellular carcinoma[J]. Molecular cancer therapeutics,2017,16(12):2711-2723.
[18]LUO Y,ROY M,XIAO X,et al. Lycorine induces programmed necrosis in the multiple myeloma cell line ARH-77[J]. Tumor biology,2015,36(4):2937-2945.
[19]WUYI L,QIAN Z,QIN T,et al. Lycorine inhibits cell proliferation and migration by inhibiting ROCK1/cofilin-induced actin dynamics in HepG2 hepatoblastoma cells[J]. Oncology reports,2018,40(4):2298-2306.

相似文献/References:

[1]抗晶晶,李桂兰,王 期,等.石蒜碱对内毒素致炎症小鼠体内体外COX-2的影响[J].南京师范大学学报(自然科学版),2016,39(01):86.
 Kang Jingjing,Li Guilan,Wang Qi,et al.The Effect of Lycorine on LPS-Induced COX-2 in vivo and vitro[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(04):86.

备注/Memo

备注/Memo:
收稿日期:2018-10-27.
基金项目:河南省科技厅科技攻关项目(172102310208).
通讯联系人:抗晶晶,硕士,讲师,研究方向:中药抗肿瘤抗炎机制研究. E-mail:kangjingjingkuaile@163.com
更新日期/Last Update: 2019-12-31