[1]王 洁,郭浩君,张晨岭.苏姜猪线粒体基因组全长测序及线粒体基因多样性分析[J].南京师范大学学报(自然科学版),2019,42(04):97-102.[doi:10.3969/j.issn.1001-4616.2019.04.014]
　Wang Jie,Guo Haojun,Zhang Chenling.Whole Genome Sequencing and Genetic Diversity ofMitochondrial Genome of Sujiang Pig[J].Journal of Nanjing Normal University(Natural Science Edition),2019,42(04):97-102.[doi:10.3969/j.issn.1001-4616.2019.04.014]
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苏姜猪线粒体基因组全长测序及线粒体基因多样性分析()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第42卷
期数:
2019年04期
页码:
97-102
栏目:
·生命科学·
出版日期:
2019-12-30

文章信息/Info

Title:
Whole Genome Sequencing and Genetic Diversity ofMitochondrial Genome of Sujiang Pig
文章编号:
1001-4616(2019)04-0097-06
作者:
王 洁1; 郭浩君3; 张晨岭2; 3
(1.江苏农牧科技职业学院,江苏 泰州 225300)(2.南京农业大学动物科技学院,江苏 南京 210095)(3.江苏第二师范学院生命科学与化学化工学院,江苏 南京 210013)
Author(s):
Wang Jie1; Guo Haojun3; Zhang Chenling2; 3
(1.Jiangsu Vocational College of Agriculture and Animal Husbandry,Taizhou 225300,China)(2.College of Animal Science and Technology,Nanjing Agricultural University,Nanjing 210095,China)(3.College of Life Science,Chemistry and Chemical Engineering,Jiangsu
关键词:
苏姜猪; 线粒体基因组; 遗传多样性; 系统发育
Keywords:
Sujiang pig; mitochondrial genome; genetic diversity; phylogeny
分类号:
S828.8
DOI:
10.3969/j.issn.1001-4616.2019.04.014
文献标志码:
A
摘要:
从分子水平上探究苏姜猪的遗传多样性,采用二代测序方法测定了苏姜猪线粒体基因组全序列,整合 NCBI 数据库中已公布的家猪类,以及本实验室测定的苏姜猪线粒体基因组全序列,使用蛋白编码基因和 rRNA 基因联合的数据集,基于贝叶斯法(BI法)和最大似然法(ML法)构建了家猪类的系统发生树,测序结果表明,该样本线粒体基因组全长15 436 bps,包含典型的家猪类线粒体基因组37条基因,结构紧凑,其线粒体基因组碱基组成(A+T)约占40%,(C+G)约占60%,tRNA总共有8处错配,结果表明苏姜猪具有丰富的遗
Abstract:
To discover the genetic diversity of Sujiang pig at molecular level,the complete mitochondrial genome of Sujiang pig was sequenced by next generation sequencing. The Sujiang pig had high genetic diversity in 15436 bps 37 genes,which A+T estimated percentage of 40%,C+G estimated percentage of 60% of its mitochondrial genome and tRNA had 8 mismatches. The Jianghai type mitochondrial nucleotide polymorphism was higher than the average level of the all six pig types,which revealed its unique genetic diversity. Phylogenetic analysis based on the complete coding region of the mitochondrial genome using NJ method,Sujiang pig was a separate pig among the six types of domestic pigs in China,and support its unique pig pedigree.

参考文献/References:

[1] 农业部公告第1984号,http://ceshi.moa.gov.cn/fw/ggb/201308/t20130830_3592384.htm.
[2]《中国家畜家禽品种志》编委会/《中国猪品种志》编写组. 中国猪品种志[M]. 上海:上海科学技术出版社,1986.
[3]DRUMMOND A J,ASHTON B,CHEUNG M,et al. Geneious v4.8.5,http://www.geneious.com.
[4]BERNT M,DONATH A,JüHLING F,et al. MITOS:Improved de novo metazoan mitochondrial genome annotation[J]. Molecular phylogenetics and evolution,2013,69(2):313-319.
[5]LARKIN M A,BLACKSHIELDS G,BROWN N P,et al. Clustal W and Clustal X version 2.0[J]. Bioinformatics,2007,23:2947-2948.
[6]ROZAS J,FERRER M A,SáNCHEZ D,et al. DnaSP 6:DNA sequence polymorphism analysis of large datasets[J]. Mol Biol Evol,2017,34:3299-3302.
[7]KUMAR S,STECHER G,TAMURA K. MEGA7:molecular evolutionary genetics analysis version 7.0 for bigger datasets[J]. Molecular biology & evolution,2016,33(7):1870.
[8]张亚平,施立明. 动物线粒体DNA多态性的研究概况[J]. 动物学研究,1992,13(3):289-298.
[9]黄勇富,张亚平,邱祥聘,等. 猪线粒体DNA多态性与中国地方猪种起源分化的关系[J]. Journal of genetics & genomics,1998,25(4):322-329.
[10]胡慧艳,贾青,刘津,等. 中国地方猪种线粒体基因组遗传多样性及其系统发育分析[J]. 中国畜牧兽医,2017,44(8):2360-2368.

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备注/Memo

备注/Memo:
收稿日期:2019-03-19.
基金项目:国家发改委资助项目(11710015021).
通讯联系人:王洁,讲师,研究方向:动物遗传多样性. E-mail:dearwj1980@163.com
更新日期/Last Update: 2019-12-31