[1]钟言欣,刘 燕,李 磊,等.土壤中的Cd对抗虫转基因水稻种植的影响[J].南京师大学报(自然科学版),2023,46(02):140-148.[doi:10.3969/j.issn.1001-4616.2023.02.018]
　Zhong Yanxin,Liu Yan,Li Lei,et al.Effect of Soil Cd on Insect-Resistant Transgenic Rice Planting[J].Journal of Nanjing Normal University(Natural Science Edition),2023,46(02):140-148.[doi:10.3969/j.issn.1001-4616.2023.02.018]
点击复制

土壤中的Cd对抗虫转基因水稻种植的影响()
分享到：

《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第46卷
期数:
2023年02期
页码:
140-148
栏目:
环境科学与工程
出版日期:
2023-06-15

文章信息/Info

Title:
Effect of Soil Cd on Insect-Resistant Transgenic Rice Planting
文章编号:
1001-4616(2023)02-0140-09
作者:
钟言欣1; 刘 燕2; 李 磊3; 秦红益1; 韩 成3; 刘 标4
(1.南京师范大学中北学院,江苏 丹阳 212332)
(2.苏州市百信环境检测工程技术有限公司,江苏 苏州 215101)
(3.南京师范大学地理科学学院,江苏 南京 210023)
(4.生态环境部南京环境科学研究所,江苏 南京 210042)
Author(s):
Zhong Yanxin1; Liu Yan2; Li Lei3; Qin Hongyi1; Han Cheng3; Liu Biao4
(1.Zhongbei College,Nanjing Normal University,Danyang 212332,China)
(2.Suzhou Baixin Environmental Testing Engineering Technology Company Limited,Suzhou 215101,China)
(3.School of Geography,Nanjing Normal University,Nanjing 210023,China)
(4.Nanjing Institute of Environmental Science,Ministry of Ecology and Environment,Nanjing 210042,China)
关键词:
转基因水稻; 镉胁迫; 氧化酶系活性; Cd富集
Keywords:
insect-resistant transgenic rice;  cadmium stress;  oxidase activity;  Cd accumulation
分类号:
X173
DOI:
10.3969/j.issn.1001-4616.2023.02.018
文献标志码:
A
摘要:
外源抗虫基因转入有利于作物减少药肥施用、降低虫害,其环境适应性研究具有生态学意义. 当前,抗虫转基因作物对重金属胁迫的适应潜力尚不清楚. 本研究以抗虫转基因水稻(HH1)及其非转基因亲本水稻(MH63)为研究对象,设置添加量分别为0、3.5、60和240 mg·kg-1的土壤Cd(II)胁迫进行水稻盆栽实验,测定水稻分蘖期和成熟期农艺性状、各植株器官Cd含量、水稻根系氧化酶系活性和根际土壤理化性质. 结果显示,与Cd0相比,Cd240处理下HH1与MH63水稻株高分别降低35%、46%; 地上生物量分别降低59%、73%; 产量分别降低55%、85%. Cd胁迫能明显降低水稻株高、生物量及产量. 相同Cd胁迫下,HH1水稻的株高、生物量及产量高于MH63水稻,说明外源Bt基因的转入能够提高水稻对Cd胁迫的抗性. 外源Bt基因的转入没有改变Cd在水稻中的积累模式,但是相同Cd胁迫下,HH1水稻各器官中Cd含量高于MH63水稻. 丙二醛(MDA)含量结果表明,分蘖期HH1水稻具有比MH63水稻更好的抗Cd能力,而成熟期则相反. 其原因可能与植株器官Cd含量、根系微环境、解毒酶系统有关. 综上所述,Cd胁迫与外源基因转入会对水稻农艺性状及各器官Cd富集产生影响,分蘖期HH1水稻具有比MH63水稻更强的抗Cd能力.
Abstract:
The modification of insect-resistant gene is conducive to reduce insect pests and decrease pesticide and fertilizer application. Thus, the research on environmental adaptability of insect-resistant transgenic crops has important ecological significance. However, the adaptation potential of insect-resistant transgenic crops to heavy metal stress remains unclear. In this study, insect-resistant transgenic rice Huahui 1(HH1)and its non-transgenic parent rice Minghui 63(MH63)are selected for a pot experiment, and the soil Cd(II)concentrations are set as 0, 3.5, 60 and 240 mg·kg-1, respectively. The agronomic traits, Cd content in plant organs, enzyme activity of rice roots and leafs, as well as physical and chemical properties of rhizosphere soil, are determined at tillering stage and maturity stage. The results show that compared with Cd0, the plant height of HH1 and MH63 rice under Cd240 treatment decrease by 35% and 46%, respectively. Their aboveground biomass decrease by 59% and 73% respectively, and their yield is reduced by 55% and 85%, respectively. Cd stress can significantly reduce plant height, biomass and yield of rice. Under the same Cd stress, the plant height, biomass and yield of HH1 rice are higher than those of MH63 rice, which indicate that the modification of exogenous Bt gene could improve the resistance of rice to Cd stress. The modification of exogenous Bt gene don't change the accumulation pattern of Cd in rice, however, under the same Cd stress, the Cd content in each organs of HH1 rice is higher than that of MH63 rice. The results of malondialdehyde(MDA)content showe that HH1 rice have better Cd resistance than MH63 rice at tillering stage, and the opposite was at maturity stage. The reason might be related to Cd concentration in plant organs, root microenvironment and detoxification enzyme system.In conclusion, Cd stress and exogenous gene modification affecte rice agronomic traits and Cd enrichment in plant organs. Moreover, HH1 rice at tillering stage have stronger Cd resistance than MH63 rice.

参考文献/References:

[1]MC LAUGHLIN M J,PARKER D R,CLARKE J M. Metals and micronutrients-food safety issues[J]. Field crops research,1999,60(1/2):143-163.
[2]LIU J,LI K,XU J,et al. Lead toxicity,uptake,and translocation in different rice cultivars[J]. Plant science,2003,165(4):793-802.
[3]JACKSON A P,ALLOWAY B J. The transfer of cadmium from agricultural soils to the human food chain[J]. Biogeochemistry of trace metals,2017:121-170.
[4]DICKINSON N M,LEPP N W. Metals and trees:impacts,responses to exposure and exploitation of resistance traits[C]//Contaminated soils:3rd International Conference on the Biogeochemistry of Trace Elements. Paris,France,1997,85:247-254.
[5]DONG J,MAO W H,ZHANG G P,et al. Root excretion and plant tolerance to cadmium toxicity — a review[J]. Plant soil and environment,2007,53(5):193-200.
[6]王宵宵,钟文辉,李磊,等. 铜胁迫对抗虫转基因水稻根际土壤微生物的影响[J]. 土壤,2020,52(1):119-126.
[7]史静,潘根兴,夏运生,等. 镉胁迫对两品种水稻生长及抗氧化酶系统的影响[J]. 生态环境学报,2013,22(5):832-837.
[8]ALI Q,SHABAAN M,ASHRAF S,et al. Genetically modified rice stacked with antioxidants for nutrient enhancement and stress tolerance[M]//Rice research for quality improvement:genomics and genetic engineering. Singapore:Springer,2020.
[9]贾茵,刘才磊,兰晓悦,等. 镉胁迫对小报春幼苗生长及生理特性的影响[J]. 西北植物学报,2020,40(3):454-462.
[10]MUNGAI N W,MOTAVALLI P P,NELSON K A,et al. Differences in yields,residue composition and N mineralization dynamics of Bt and non-Bt maize[J]. Nutrient cycling in agroecosystems,2005,73(1):101-109.
[11]WANG Y M,ZHANG G,DU J,et al. Influence of transgenic hybrid rice expressing a fused gene derived from cry1Ab and cry1Ac on primary insect pests and rice yield[J]. Crop protection,2010,29(2):128-133.
[12]XIA H,CHEN L Y,WANG F,et al. Yield benefit and underlying cost of insect-resistance transgenic rice:Implication in breeding and deploying transgenic crops[J]. Field crops research,2010,118(3):215-220.
[13]CHEN M,ZHAO J Z,GONG YIN Y E,et al. Impact of insect-resistant transgenic rice on target insect pests and non-target arthropods in China[J]. Insect science,2006,13(6):409-420.
[14]CLARK B W,PHILLIPS T A,COATS J R. Environmental fate and effects of Bacillus thuringiensis(Bt)proteins from transgenic crops:a review[J]. Journal of agricultural and food chemistry,2005,53(12):4643-4653.
[15]谢小波,舒庆尧. 用Envirologix Cry1Ab/Cry1Ac试剂盒快速测定转基因水稻Bt杀虫蛋白含量的研究[J]. 中国农业科学,2001,34(5):465-468.
[16]陈希. 茶园根际土壤与植物体养分对酸沉降的响应[D]. 江西:南昌大学,2015.
[17]HAN C,LIU B,ZHONG W. Effects of transgenic Bt rice on the active rhizospheric methanogenic archaeal community as revealed by DNA-based stable isotope probing[J]. Journal of applied microbiology,2018,125(4):1094-1107.
[18]XU J,HAN C,JIANG Y B,et al. Spatial distribution and co-occurrence of aerobic ammonia oxidation and anaerobic ammonium oxidation activities in the water-soil interface,bulk,and rhizosphere regions of paddy soil[J]. Plant and soil,2021,466(1):557-568.
[19]郭继斌,王莉,韩娇,等. 联合浸提法测定土壤有效态镉[J]. 江苏农业科学,2016,44(3):369-372.
[20]肖家欣. 植物生理学实验[M]. 安徽:安徽人民出版社,2010.
[21]WEI Z,HONG F,MING Y,et al. Subcellular and molecular localization of rare earth elements and structural characterization of yttrium bound chlorophyll a in naturally grown fern Dicranopteris dichotoma[J]. Microchemical journal,2005,80(1):1-8.
[22]张锡洲,张洪江,李廷轩,等. 水稻镉耐性差异及镉低积累种质资源的筛选[J]. 中国生态农业学报,2013,21(11):1434-1440.
[23]黄冬芬. 水稻对土壤重金属镉的响应及其调控[D]. 扬州:扬州大学,2008.
[24]宋新华,镉和镉+高温对双抗氧化酶转基因水稻生长生理和抗氧化系统的影响[D]. 山东:山东师范大学,2008.
[25]段苏然,施卫明,王俊儒. 过量表达pAPX基因提高水稻对镉胁迫的耐性[J]. 土壤学报,2006,1:111-116.
[26]WANG F,YE C,ZHU L Y,et al. Yield differences between Bt transgenic rice lines and their non-Bt counterparts,and its possible mechanism[J]. Field crops research,2012,126:8-15.
[27]朱超,文美兰,刘攀峰,等. 桂林灵川县典型有机水稻田重金属元素分布特征及污染评价[J]. 现代地质,2021,35(5):1433-1440.
[28]李玲,陈进红,何秋伶,等. 3个陆地棉种质(系)重金属镉的积累、转运和富集特性分析[J]. 棉花学报,2012,24(6):535-540.
[29]中华人民共和国国家卫生健康委员会,国家市场监督管理总局. 食品安全国家标准 食品中污染物限量:GB 2762-2022[S]. 北京:中国标准出版社,2022.
[30]彭鸥,叶长城,刘玉玲,等. Cd胁迫下水稻叶片SOD活性和MDA含量与糙米中Cd含量的相关性研究[J]. 生态毒理学报,2019,14(6):233-240.
[31]章秀福,王丹英,储开富,等. 镉胁迫下水稻SOD活性和MDA含量的变化及其基因型差异[J]. 中国水稻科学,2006,20(2):194-198.
[32]任新宇,罗晟,魏宏宇,等. 酸性环境对镉胁迫下水稻幼苗生长的影响[J]. 河南农业科学,2022,51(4):39-47.
[33]霍洋,仇银燕,周航,等. 外源磷对镉胁迫下水稻生长及镉累积转运的影响[J]. 环境科学,2020,41(10):4719-4725.
[34]郑沈,黄道友,李波,等. 外源Fe调控根系微生物群落结构和功能对水稻Cd积累的影响[J]. 环境科学,2022,43(8):4313-4321.
[35]史静,潘根兴,夏运生,等. 镉胁迫对两品种水稻生长及抗氧化酶系统的影响[J]. 生态环境学报,2013,22(5):832-837.

备注/Memo

备注/Memo:
收稿日期:2022-11-13.
基金项目:农业部转基因生物新品种培育重大专项(2016ZX08012005)、国家自然科学基金项目(42077033).
通讯作者:秦红益,博士,讲师,研究方向:环境微生物学研究. E-mail:qinhongyii@163.com
更新日期/Last Update: 2023-06-15