[1]程罗根,许勤,于光,等.杀虫剂的选择作用对小菜蛾表达蛋白质的影响[J].南京师大学报(自然科学版),2006,29(04):101-104.
　Cheng Luogen~,Xu Qin~,Yu Guang~,et al.Protein Changes in Response to Insecticides Selection Pressure in Diamondback Moth[J].Journal of Nanjing Normal University(Natural Science Edition),2006,29(04):101-104.
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杀虫剂的选择作用对小菜蛾表达蛋白质的影响()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第29卷
期数:
2006年04期
页码:
101-104
栏目:
生命科学
出版日期:
2006-12-30

文章信息/Info

Title:
Protein Changes in Response to Insecticides Selection Pressure in Diamondback Moth
作者:
程罗根;许勤;于光;李忠英;
南京师范大学生命科学学院
Author(s):
Cheng Luogen~1;  Xu Qin~2;  Yu Guang~1; Li Zhongying~3
1.School of Life Science, Nanjing Normal University, Nanjing 210097, China)
关键词:
小菜蛾;  抗药性;  蛋白质;  双向电泳
分类号:
S482.3
摘要:
运用蛋白质双向电泳分析技术,通过对成虫蛋白质的提取,分离出水溶性的蛋白质组分,对比研究了溴氰菊酯抗性品系、杀螟丹抗性品系、杀虫双抗性品系和敏感品系小菜蛾成虫的蛋白质在表达上的差异.通过对电泳图谱分析,在溴氰菊酯的抗性和敏感品系中获得了8个特异的蛋白质斑点,pH5·0~8·1,相对分子质量为46000~23000;在抗杀螟丹品系中得到了8个特异斑点,pH4·6~8·1,相对分子质量为92000~18000;在杀虫双的抗性品系和敏感品系中产生了6个特异斑点,pH5·5~7·1,相对分子质量为31000~28000.虽然抗性品系是敏感品系通过多年的室内逐代的药剂选择获得的,但是在杀虫剂选择压力作用下所引起的表达蛋白质的差异是否与溴氰菊酯、杀螟丹和杀虫双的抗性有关还需进一步证实.

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备注/Memo

备注/Memo:
江苏省高校自然科学基金资助项目(03KJD180112).
更新日期/Last Update: 2013-05-05