[1]刘丽平,薛晖,周刚,等.复方中草药对中华绒螯蟹免疫因子的影响[J].南京师大学报(自然科学版),2008,31(01):109-113.
　Liu Liping,Xue Hui,Zhou Gang.Effect of Chinese Herbal Medicine on Immunity Function of Eriocheir Sinensis[J].Journal of Nanjing Normal University(Natural Science Edition),2008,31(01):109-113.
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复方中草药对中华绒螯蟹免疫因子的影响()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第31卷
期数:
2008年01期
页码:
109-113
栏目:
生命科学
出版日期:
2008-03-30

文章信息/Info

Title:
Effect of Chinese Herbal Medicine on Immunity Function of Eriocheir Sinensis
作者:
刘丽平;薛晖;周刚;
江苏省淡水水产研究所, 江苏南京210017
Author(s):
Liu Liping;  Xue Hui;  Zhou Gang
Jiangsu Institute of Freshwater Fisheries, Nanjing 210017, China
关键词:
中华绒螯蟹;  复方中草药;  非特异性免疫;  免疫指标
Keywords:
E rioche ir sinensis;  Chinese herba l com pound;  non-spec ific immunity;  immunity indices
分类号:
S945
摘要:
在基础饲料中添加6种中草药制剂煎液制成试验饲料,同时以基础饲料为对照,连续饲喂中华绒螯蟹30d,采集中华绒螯蟹血淋巴上清液测定其免疫指标活性.T检验结果表明,中草药添加饲料组蟹的噬菌活性与对照组存在显著差异(P<0.05);溶菌酶活性、酚氧化酶活性(PO)与对照组则差异极显著(P<0.01);抗菌酶活性、过氧化物酶活性(POD)差异不显著(P>0.05),但均值比对照饲料组高.说明该中草药制剂的添加能明显提高中华绒螯蟹的免疫水平.用中华绒螯蟹致病菌嗜水气单胞菌CL99920对实验蟹进行攻毒试验, 嗜水气单胞菌的最佳攻毒剂量为5 × 106 cfu /mL, 对照组的死亡率为80%, 明显高于中草药免疫组的死亡率30%, 该中草药添加剂的免疫保护率为62. 5%.
Abstract:
S ix ind ices o f non-specific imm un ityw erem easured a fter Er ioche ir sinensis were fed w ith Chinese he rbal com pound as add itive to norm a l food for 30 day s. There w ere s ignificant differences on phagocy tic activ ities between experi m enta l and contro l g roup by usingT test( P < 0. 05). Therew ere h ighly sign ificant d iffe rences on bacte rio lytic and phe nolyx idase activ ities. But therew ere no s ignificant d ifferences on antibacter ia l and POD activ ities. These resu lts indica ted that the Ch inese herbal m ed ic ine m ight sign ificantly elevate the non-spec ific immunity o f c rab. The Ch inese herbal m ed icine used in the fie lds could effic iently prev ent the occurrence of crab disease and increase the surv iva l ra te o f crab compared w ith the contro l g roup a fter the crabs w ere attacked w ith Aerom ans tydroph ila CL99920.

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备注/Memo

备注/Memo:
基金项目:国家十一五科技支撑计划“水产名特优品种优质高效养殖技术研究与示范”(2006BAD03B07)资助项目.
作者简介: 刘丽平( 1976- ) , 女, 研究实习员, 硕士, 研究方向: 水产动物疾病学. E-m ail:hongb ingyu@ s ina. com
更新日期/Last Update: 2013-05-05