[1]沈宇清,夏梅,缪凤琴,等.PML分子不同剪接体的分型及其应用[J].南京师大学报(自然科学版),2009,32(03):88-91.
　Shen Yuqing,Xia Mei,Miao Fengqing,et al.Isolation Different PML Isoform by PCR Analysis[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(03):88-91.
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PML分子不同剪接体的分型及其应用()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第32卷
期数:
2009年03期
页码:
88-91
栏目:
生命科学
出版日期:
2009-09-30

文章信息/Info

Title:
Isolation Different PML Isoform by PCR Analysis
作者:
沈宇清;夏梅;缪凤琴;谢维;张建琼;
东南大学医学院遗传与发育生物学系, 发育与疾病相关基因教育部重点实验室, 江苏南京210009
Author(s):
Shen Yuqing; Xia Mei; Miao Fengqing; Xie Wei; Zhang Jianqiong
The Key Laboratory of Developmental Genes and Human Disease,Ministry of Education,Department of Genetics and Developmental Biology,Southeast University Medical School,Nanjing 210009,China
关键词:
PML基因;  剪接体;  PCR
Keywords:
PML gene;  iso form;  PCR
分类号:
R733.7
摘要:
早幼粒细胞白血病(Promyelocytic Leukemia,PML)基因位于15号染色体,含有9个外显子,通过对内含子不同的剪接方式产生7大类20余种不同的PML剪接体.为对PML不同剪接体进行分型,针对PML各剪接体C末端设计5对型特异性引物,通过PCR扩增产物的有无及大小来判断PML分子的型别.利用设计的引物,通过对已知类型的PML质粒进行分型,确定了分型引物的可行性.对一株肝癌细胞系QGY7701中表达的PML分子进行分型,发现其为PML剪接体IV型与II型的混合型.研究表明,利用所设计5对引物的扩增, 可以判断出某种组织或细胞中表达的PML分子剪接体型别.
Abstract:
PM L ( Prom yelocy tic Leukem ia) gene is located on chrom osom e 15 and com po sed of n ine exons. Mo re than twenty d ifferen t PML iso form s have been discovered and categorized in to seven g roups by diffe rent splic ing pattern. In order to indentify different isoforms o f the ind iv idua l g roups, five pa irs of prim er were designed to be used for iso form-spec ific PCR am plification acco rd ing to the sequences at theC-term ina l o f PML gene. Analysis of the diffe rent iso form s w as based on the presence and the s ize o f the PCR product am plified by using a ll the five pairs of pr im ers. Contro l exper-i m ent w as done by analysis o f a pc DNA3.1-GFP-PML plasm id wh ich is known to be PMLIV. In add ition, PML iso form in a liver cancer ce ll line QGY7701 w as determ ined to be a m ixture of PMLII and PM LIV by the sam e way. In conc lus ion, PML isoform o f the seven g roups can be c lassified by sim ply PCR amp lification using the des igned five pa irs o f pr im ers.

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备注/Memo

备注/Memo:
基金项目: 国家自然科学基金(编号30371311) .
通讯联系人: 张建琼, 博士, 教授, 研究方向: 免疫遗传学与病原生物学. E-m ail:zh jq@ seu. edu. cn
更新日期/Last Update: 2013-04-23