[1]李霖,尹萍,季金强,等.骨形态发生蛋白7在兔卵巢中的表达分析[J].南京师大学报(自然科学版),2009,32(04):94-97.
 Li Lin,Yin Ping,Ji Jinqiang,et al.Expression and Discussion of Bone Morphogenetic Protein 7 in the Rabbit Ovary[J].Journal of Nanjing Normal University(Natural Science Edition),2009,32(04):94-97.
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骨形态发生蛋白7在兔卵巢中的表达分析()
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《南京师大学报(自然科学版)》[ISSN:1001-4616/CN:32-1239/N]

卷:
第32卷
期数:
2009年04期
页码:
94-97
栏目:
生命科学
出版日期:
2009-12-30

文章信息/Info

Title:
Expression and Discussion of Bone Morphogenetic Protein 7 in the Rabbit Ovary
作者:
李霖;尹萍;季金强;丁家桐;
扬州大学动物科学与技术学院, 江苏扬州225009
Author(s):
Li LinYin PingJi JinqiangDing Jiatong
College of Animal Science and Technology,Yangzhou University,Yangzhou 225009,China
关键词:
骨形态发生蛋白7 卵巢 免疫组织化学
Keywords:
rabbit bone m orphogene tic pro te in7( BMP7) ov ary imm unoh istochem istry
分类号:
Q492
摘要:
为探讨BM P7蛋白在卵泡发育、黄体化和排卵过程中的作用, 本研究采用免疫组织化学方法检测了 BM P7在兔卵巢中的表达定位情况. 结果显示, BM P7蛋白在兔卵巢原始卵泡和初级卵泡中未检测到表达; 在次级卵泡卵母细胞及有腔卵泡的各种细胞中都有表达, 但只在部分内膜细胞中表达; 在间质细胞、黄体及表面上皮中也有表达. 由此表明, BM P7没有参与兔原始卵泡的激活, 而在初级/次级卵泡转化以及其后的卵泡成熟过程中发挥了作用; BMP7不仅是卵泡内膜细胞分泌因子, 而且也是卵母细胞和颗粒细胞分泌因子. 对BMP7的表达来说卵泡内膜细胞存在功能性差异. BMP7可能参与了兔黄体、排卵等活动的调节.
Abstract:
To discuss the ac tions of bonem o rphog enetic prote in 7 ( BMP7) in the process o f fo llicu lar deve lopm ent, lute in ization and ovu lateon, w e exam ined the localization of BMP7 prote in in the rabbit ov ary using imm unohistochem ica l m e thod. The results showed that BMP7 pro te in w as no t expressed in pr imo rdia l or prim ary fo llic les. Instead, it w as found in both oocytes o f secondary fo llic les and all ce lls of antral fo llic les. It was a lso detected in interstitia l ce lls, corpora lutea and ovarian surface epithelia l cells. These suggested tha t BMP7 did not take pa rt in the activation of a do rm ant prim ord ial follic le, but p layed a ro le in the pr im ary / secondary transition and in the fo llow ing m atura tion o f fo llic les. Besides, BM P7 w as not only the cytokine of theca inte rna ce lls but a lso tha t o f oocytes and granulosa cells, BMP7 m ight a-l so partic ipate in the regulatory activ ities o f co rpora lutea, ovulation, e tc.

参考文献/References:

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备注/Memo

备注/Memo:
基金项目: 江苏省自然科学基金( BK200505)资助项目.
通讯联系人: 丁家桐, 教授, 博士生导师, 研究方向: 动物生殖与调控研究. E-ma il:jtd ing @ yzu. edu. cn
更新日期/Last Update: 2013-04-23