[1]张雯翔,许　琦,陈思禹,等.PGC-1β拮抗LPS诱导的肝脏Hepcidin表达升高及铁过度堆积[J].南京师范大学学报(自然科学版),2016,39(03):67.[doi:10.3969/j.issn.1001-4616.2016.03.012]
　Zhang Wenxiang,Xu Qi,Chen Siyu,et al.PGC-1β Antagonizes LPS-Induced Hepcidin Expressionand Iron Accumulation in Liver[J].Journal of Nanjing Normal University(Natural Science Edition),2016,39(03):67.[doi:10.3969/j.issn.1001-4616.2016.03.012]
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PGC-1β拮抗LPS诱导的肝脏Hepcidin表达升高及铁过度堆积()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第39卷
期数:
2016年03期
页码:
67
栏目:
·生命科学·
出版日期:
2016-09-30

文章信息/Info

Title:
PGC-1β Antagonizes LPS-Induced Hepcidin Expressionand Iron Accumulation in Liver
文章编号:
1001-4616（2016）03-0067-07
作者:
张雯翔; 许　琦; 陈思禹; 刘　畅
南京师范大学生命科学学院，江苏 南京 210023
Author(s):
Zhang Wenxiang; Xu Qi; Chen Siyu; Liu Chang
School of Life Sciences，Nanjing Normal University，Nanjing 210023，China
关键词:
PGC-1β; Hepcidin; 铁代谢; 炎症; 肝脏
Keywords:
PGC-1β; Hepcidin; iron metabolism; inflammation; liver
分类号:
Q291
DOI:
10.3969/j.issn.1001-4616.2016.03.012
文献标志码:
A
摘要:
探讨PGC-1β在调节肝脏Hepcidin表达及铁代谢稳态中的作用. 在脂多糖（Lipopolysaccharides，LPS）处理的小鼠肝脏和原代肝细胞中，通过RT-qPCR和Western Blot实验检测PGC-1β及Hepcidin的表达. 使用腺病毒介导的过表达和干扰手段，研究PGC-1β对本底及LPS刺激下Hepcidin表达的影响. 最后，通过普鲁士蓝染色法探究PGC-1β对LPS刺激后肝脏铁离子堆积的影响. 结果表明：（1）LPS在体内和体外水平，均可显著抑制PGC-1β的表达，而增加Hepcidin的表达；（2）过表达PGC-1β能抑制LPS诱导的Hepcidin表达及胞内铁堆积. 综上，PGC-1β可以拮抗LPS诱导的Hepcidin表达升高及铁的过度堆积.
Abstract:
This study is aimed to investigate the effect of PGC-1β on LPS-induced Hepcidin expression and iron accumulation in liver. RT-qPCR and Western Blot analyses were used to detect the expression levels of PGC-1β and Hepcidin in mouse liver and primary hepatocytes treated with LPS. Gain- and loss-of-function studies were performed to assess the role of PGC-1β in the regulation of Hepcidin expression under basal and LPS-stimulated conditions. Finally，Perls’ Prussian blue staining was administrated to detect the effects of PGC-1β on LPS-induced hepatic iron deposition. Results showed that，（1）LPS caused a significant reduction in PGC-1β expression，whereas increased Hepcidin expression both in vivo and in vitro；（2）Over-expression of PGC-1β inhibited LPS-induced Hepcidin expression and intracellular iron accumulation in liver. PGC-1β antagonizes LPS-induced Hepcidin expression and iron accumulation in liver.

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备注/Memo

备注/Memo:
收稿日期：2016-04-14.　
基金项目：国家自然科学基金委优秀青年科学基金（31422028）、面上项目（31271261）、江苏省杰出青年基金（BK20140041）.　
通讯联系人：刘畅，博士，教授，研究方向：能量代谢调控的分子机制. E-mail：changliu@njnu.edu.cn
更新日期/Last Update: 2016-09-30