[1]孙 宾,刘莹莹,李 鹏,等.中华绒螯蟹与长江华溪蟹胚胎发育相关miRNA识别与比较分析[J].南京师范大学学报(自然科学版),2017,40(02):65.[doi:10.3969/j.issn.1001-4616.2017.02.011]
　Sun Bin,Liu Yingying,Li Peng,et al.Characterization and Comparative Analysis of MicroRNAs During EmbryonicDevelopment Between the Chinese Mitten Crab(Eriocheir sinensis)and the Freshwater Crab(Sinopotamon yangtsekiense)[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(02):65.[doi:10.3969/j.issn.1001-4616.2017.02.011]
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中华绒螯蟹与长江华溪蟹胚胎发育相关miRNA识别与比较分析()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年02期
页码:
65
栏目:
·生命科学·
出版日期:
2017-06-29

文章信息/Info

Title:
Characterization and Comparative Analysis of MicroRNAs During EmbryonicDevelopment Between the Chinese Mitten Crab(Eriocheir sinensis)and the Freshwater Crab(Sinopotamon yangtsekiense)
文章编号:
1001-4616(2017)02-0065-07
作者:
孙 宾; 刘莹莹; 李 鹏; 严 洁; 周开亚
南京师范大学生命科学学院,江苏省生物多样性与生物技术重点实验室与江苏省水生甲壳动物病害重点实验室,江苏 南京 210023
Author(s):
Sun Bin; Liu Yingying; Li Peng; Yan Jie; Zhou Kaiya
School of Life Sciences,Nanjing Normal University,Jiangsu Key Laboratory for Biodiversity and Biotechnologyand Jiangsu Key Laboratory for Aquatic Crustacean Diseases,Nanjing 210023,China
关键词:
中华绒螯蟹; 长江华溪蟹; 小RNA; miRNA
Keywords:
Eriocheir sinensis; Sinopotamon yangtsekiense; microRNA; miRNA
分类号:
Q951+4
DOI:
10.3969/j.issn.1001-4616.2017.02.011
文献标志码:
A
摘要:
利用Illumina/Solexa高通量测序技术,开展了中华绒螯蟹和长江华溪蟹胚胎的小RNA深度测序. 对测序结果进行生物信息学分析,分别筛选获得中华绒螯蟹的8 569 743条和长江华溪蟹的8 719 465条干净序列(Clean Unique Reads),长度分布在20 nt～22 nt区间内的分别占有30.47%(Ejs-OB:中华绒螯蟹胚胎测序文库)和30.38%(SY-OJC:长江华溪蟹胚胎测序文库). 用SOAP程序将小RNA定位到基因组,结果Ejs-OB中分析筛选出5 745 279个小RNA,其中13 472种小RNA与基因组的序列匹配; SY-OJC中分析筛选出4 397 509个小RNA,其中18 407种小 RNA与基因组的序列匹配. 分类注释的结果显示,中华绒螯蟹和长江华溪蟹分别有6 293 445(Ejs-OB)和5 596 614(SY-OJC)条miRNA候选序列,但未预测发现新miRNA. 在表达水平的差异分析结果中发现miR-1183、miR-1357、 miR-1591、miR-2382的表达水平上调且差异显著. 在两文库中搜寻到一些共同miRNA*序列,其中miR-139*、miR-1419g*、miR-1798*、miR-202*、miR-2068*和 miR-454*也是表达变异较大. 这些结果表明,miRNA可能在调控与中华绒螯蟹和长江华溪蟹胚胎发育相关的基因表达中起重要作用.
Abstract:
The deep sequencing of small RNA in Eriocheir sinensis and Sinopotamon yangtsekiense embryo samples was performed using the Illumina/Solexa high-throughput sequencing technologies. After analyzing the biological information of the sequencing results,8 569 743 and 8 719 465 clean unique sequence data were screened in E.sinensis and S.yangtsekiense respectively,and the length distribution in the range of 20 nt-22 nt was 30.47%(Ejs-OB:E.sinensis embryonic sequencing library)and 30.38% respectively(SY-OJC:S.yangtsekiense embryonic sequencing library). Mapping the small RNA tags to genome by SOAP to analyze their distribution on the genome,5 745 279 and 4 397 509 small RNAs of Ejs-OB and SY-OJC were screened respectively,13 472(Ejs-OB)and 18 407(SY-OJC)of which were genome-matched reads. Annotated small RNA tags with different kinds of RNA,the results showed that a total of 6 293 445 and 5 596 614 miRNA candidate reads were searched in Ejs-OB and SY-OJC respectively. However,the novel miRNAs were not found. Expression profile analysis found that the expression level of miR-1183,miR-1357,miR-1591,miR-2382 were up-regulated and the difference were significant. Interestingly,some same miRNAs* sequences were also searched in the two library,and miR-139*,miR-1419g*,miR-1798*,miR-454* and miR-2068* of which were also have a significant differences in expression. These results indicated that these miRNAs may be involved in regulating gene expression associated with the development of the embryo in E.sinensis and S.yangtsekiense.

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备注/Memo

备注/Memo:
收稿日期:2016-12-02.
基金项目:江苏高校优势学科建设工程资助项目(PAPD)、江苏省高校自然科学基金项目(13KJB180008、15KJD180005).
通讯联系人:李鹏,博士,副教授,研究方向:功能基因组学与进化、发育生物学. E-mail:biolipeng@163.com
更新日期/Last Update: 2017-06-30