[1]周 涛,宋方霞,黄村惠.克氏原螯虾调理产品中主要腐败菌的分离鉴定[J].南京师范大学学报(自然科学版),2017,40(04):103.[doi:10.3969/j.issn.1001-4616.2017.04.017]
　Zhou Tao,Song Fangxia,Huang Cunhui.Isolation and Identification of Specific Spoilage Organismsin Conditioning Crayfish Products[J].Journal of Nanjing Normal University(Natural Science Edition),2017,40(04):103.[doi:10.3969/j.issn.1001-4616.2017.04.017]
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克氏原螯虾调理产品中主要腐败菌的分离鉴定()
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《南京师范大学学报》（自然科学版）[ISSN:1001-4616/CN:32-1239/N]

卷:
第40卷
期数:
2017年04期
页码:
103
栏目:
·生命科学·
出版日期:
2017-12-30

文章信息/Info

Title:
Isolation and Identification of Specific Spoilage Organismsin Conditioning Crayfish Products
文章编号:
1001-4616(2017)04-0103-06
作者:
周 涛; 宋方霞; 黄村惠
南京师范大学金陵女子学院,江苏 南京 210097
Author(s):
Zhou Tao; Song Fangxia; Huang Cunhui
Ginling College,Nanjing Normal University,Nanjing 210097,China
关键词:
克氏原螯虾; 调理产品; 优势腐败菌; 分离鉴定
Keywords:
Procambarus clarkii; conditioning products; SSO; isolation and identification
分类号:
TS251.7
DOI:
10.3969/j.issn.1001-4616.2017.04.017
文献标志码:
A
摘要:
为了分离鉴定克氏原螯虾调理产品在4 ℃冷藏条件下的腐败菌菌相组成,将其在4 ℃条件下冷藏至货架期末(20 d左右),此时克氏原螯虾调理产品中菌落总数为7.05 logcfu/mL. 采用传统分离纯化、菌落形态观察、生理生化鉴定与16S rDNA序列分析方法结合,对腐败菌进行分离鉴定. 结果表明:在克氏原螯虾样品中共分离得到85株菌株,经镜检、生理生化鉴定以及16S rDNA测序得到5株典型优势菌,分别为蜡样芽孢杆菌(Bacillus cereus)、甲基营养型芽孢杆菌(Bacillus methlotro
Abstract:
In order to isolate and identify the spoilage organism from conditioning crayfish products under 4 temperature conditions,the spoilage bacteria were separated from the conditioning crayfish products stored under low temperature of 4,while the total number of colonies is 7.05 logcfu/mL in conditioning crayfish products. Isolation and identification of spoilage bacteria were performed by traditional methods of isolation and purification,colony morphology observation,physiological and biochemical identification and 16S rDNA sequence analysis. The results showed that,85 strains were isolated from the conditioning crayfish products samples and 5 strains of typical dominant bacteria obtained by microscopy,physiological and biochemical identification and 16S rDNA sequencing. Respectively,Bacillus cereus,Bacillus methylotrophicus,Lysinibacillus fusiformis,and Macrococcus caseolyticus.

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备注/Memo

备注/Memo:
收稿日期:2017-05-18.
基金项目:江苏省农业科技自主创新资金项目(CX(16)1057).
通讯联系人:周涛,博士,副教授,研究方向:食品生物技术. E-mail:zhoutao@sina.com
更新日期/Last Update: 2017-12-30