[1]王彰骄,沈 勇,许青霞.基于生物信息学分析探究食管鳞状细胞癌的关键通路、基因及免疫细胞浸润[J].南京师大学报(自然科学版),2023,46(02):77-84.[doi:10.3969/j.issn.1001-4616.2023.02.010]
　Wang Zhangjiao,Shen Yong,Xu Qingxia.Analysis of Key Pathways,Genes and Immune Cell Infiltration in Metastatic Esophageal Squamous Cell Carcinoma Based on Bioinformatics[J].Journal of Nanjing Normal University(Natural Science Edition),2023,46(02):77-84.[doi:10.3969/j.issn.1001-4616.2023.02.010]
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基于生物信息学分析探究食管鳞状细胞癌的关键通路、基因及免疫细胞浸润()
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《南京师大学报（自然科学版）》[ISSN:1001-4616/CN:32-1239/N]

卷:
第46卷
期数:
2023年02期
页码:
77-84
栏目:
生物学
出版日期:
2023-06-15

文章信息/Info

Title:
Analysis of Key Pathways,Genes and Immune Cell Infiltration in Metastatic Esophageal Squamous Cell Carcinoma Based on Bioinformatics
文章编号:
1001-4616(2023)02-0077-08
作者:
王彰骄1; 沈 勇2; 许青霞2
(1.河南中医药大学,河南中医药大学第二临床医学院,河南 郑州 450000)
(2.河南省肿瘤医院,郑州大学附属肿瘤医院医学检验科,河南 郑州 450008)
Author(s):
Wang Zhangjiao1; Shen Yong2; Xu Qingxia2
(1.Henan University of Chinese Medicine,The Second Affiliated Hospital of Henan University of TCM,Zhengzhou 450000,China)
(2.Department of Clinical Laboratory,Affiliated Cancer Hospital of Zhengzhou University & Henan Cancer Hospital,Zhengzhou 450008,China)
关键词:
食管癌; 肿瘤标志物; 免疫浸润; 生物信息学分析
Keywords:
esophageal carcinoma;  biomarker;  immune infiltration;  bioinformatics analysis
分类号:
R735.1
DOI:
10.3969/j.issn.1001-4616.2023.02.010
文献标志码:
A
摘要:
食管鳞状细胞癌是常见的恶性肿瘤之一,本研究利用生物信息学方法系统全面地探索食管癌中的差异表达基因以及免疫细胞特征. 本文从GEO数据库中获取4个食管癌数据集,进行差异和基因富集分析,并对筛选出的核心基因进一步探索. 研究共筛选出244个差异表达基因(DEGs),其中上调基因164个,下调基因80个. 基因富集分析发现,DEGs主要富集在胞外区和细胞外间隙,与肿瘤侵袭转移相关,并且与PI3K-Akt信号通路和细胞外基质(ECM)受体相互作用信号通路相关. 进一步研究发现3个核心基因(CXCL8、MMP9和MMP13)在ESCC中高表达且能促进巨噬细胞和静息树突状细胞,并抑制肥大细胞和单核细胞. 本研究筛选出的ESCC核心基因在肿瘤免疫浸润细胞中起着重要作用,有望成为ESCC潜在的肿瘤标志物且用于ESCC的诊断和治疗.
Abstract:
Esophageal squamous cell carcinoma is one of the common malignant tumors, to systematically and comprehensively explore the characteristics of differentially expressed genes and immune cells in esophageal carcinoma by bioinformatics. Four esophageal cancer data sets were obtained from GEO database, and the differences and gene enrichment were analyzed, and the selected core genes were further explored. A total of 244 differentially expressed genes(DEGs)were screened, including 164 up-regulated genes and 80 down-regulated genes, which were mainly enriched in extracellular region and extracellular space, and related to tumor invasion and metastasis. KEGG results showed that DEGs were mainly enriched in PI3K-Akt signal pathway and extracellular matrix(ECM)receptor interaction signal pathway. And it was found that three hub genes(CXCL8, MMP9 and MMP13)were highly expressed in ESCC. The results of immune infiltration showed that macrophages and resting dendritic cells increased significantly, while mast cells and monocytes decreased significantly. In conclusion, three hub genes and immune infiltrating cells may play an important role in ESCC, and are expected to become potential tumor markers of ESCC and be used in the diagnosis and treatment of ESCC.

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备注/Memo

备注/Memo:
收稿日期:2022-05-20.
基金项目:河南省医学科技攻关计划省部共建项目(SBGJ202102070).
通讯作者:许青霞,教授,研究方向:肿瘤免疫. E-mail:hnxqx12@163.com
更新日期/Last Update: 2023-06-15